Determination of phospholipid- and lipopolysaccharide-derived fatty acids as an estimate of microbial biomass and community structures in soils

Zelles, L.; Bai, Q.Y.; Rackwitz, R.; Chadwick, David R.; Beese, F.

doi:10.1007/bf00336146

Cited by 191 publications

(106 citation statements)

References 21 publications

Supporting

Mentioning

101

Contrasting

Order By: Relevance

“…These amounts corresponded well with amounts in the OF horizon of a podsolic soil under Picea abies (Zelles et al, 1995). Satured fatty acids (SATFA) were the dominant fraction in the L/F layer, yielding about 50% of the total content of EL-PLFAs, followed by the mono-unsaturated fatty acids (MUFAS, 27%), hydroxy-substituted fatty acids (PLOHS, 12%) and the polyunsaturated fatty acids (PUFAS 8%).…”

Section: Microbial Biomass and Community Structuresupporting

confidence: 66%

Chemical Communications

Omenetto¹,

Kaplan²

2012

View full text Add to dashboard Cite

Using ®eld mesocosms maintained for 6 months in an acid spruce forest, we investigated the in¯uence of microarthropods on biomass, structure and function of the soil microbial community. In the litter layer (L/F layer), the re-immigration of mesofauna into mesocosms did not signi®cantly aect substrate-induced respiration (SIR), biomass C, biomass N, biomass P, Nmineralisation or enzymes involved in N cycling. There was no eect of the mesofauna on the biomarkers for fungal biomass (ergosterol and phospholipid fatty acid 18:2o6) in the litter layer. Mesofauna activities increased microbial biomass (biomass C, N and P) in the H layer, and signi®cantly increased soil protease activity and phosphate content in the H layer. Since biomass P did not change signi®cantly when mesofauna recolonised mesocosms, they presumably aected P-mineralisation by producing Prich faeces. Discriminant analysis showed that mesofauna aected microbial N-mobilisation in the H layer. Higher protease and arginine deaminase activities in mesocosms with mesofauna may have been caused by faunal grazing on soil microorganisms. #

show abstract

Section: Microbial Biomass and Community Structuresupporting

confidence: 66%

Chemical Communications

Omenetto¹,

Kaplan²

2012

View full text Add to dashboard Cite

show abstract

“…PLFA determination was carried out by adopted method of Zelles et al [15] corresponding to ISO 29843-2 [16] used in our previous studies [17][18][19]. Briefly, total lipids were extracted from sediments using a single-phase mixture of methanol, chloroform and phosphate buffer (2:1:0.8).…”

Section: Determination Of Plfamentioning

confidence: 99%

Tourist Traffic Significantly Affects Microbial Communities of Sandstone Cave Sediments in the Protected Landscape Area “Labské Pískovce” (Czech Republic): Implications for Regulatory Measures

et al. 2018

View full text Add to dashboard Cite

Abstract:In the Protected Landscape Area "Labské pískovce" (Czech Republic), there are many sandstone caves accessible by permission only and where climbing equipment is commonly required. The tradition of visiting the caves dates back to turn of the 19th and 20th centuries, and visitors traditionally record their visits in log-books. We have gathered attendance data from 12 caves from log-books and via survey (2-year form collection period). The attendance varied from less than 10 to~650 visitors annually. Signs of inadequate behavior of visitors were observed, especially waste disposal and smoking in the caves. Sediment step-compression increased significantly the ratios of fungi/bacteria and gram-positive to gram-negative (G+/G−) phospholipid fatty acids (PLFA) as well as PLFA of actinobacteria and activities of sediment phosphatases, glucosidases, and peroxidases. The number of visitors was correlated positively with the G+/G− ratio and sediment nutrients but negatively with sediment moisture and the activity of peroxidases. The results indicate a significant effect of attendance on sediment microbial communities, most likely caused by the import of nutrients into caves. Hence, reconsideration of the cave attendance policy is recommended.

show abstract

“…The iso and anteiso acids possibly derive from gram-positive and sulphate-reducing bacteria in the mats (Grimalt et al, 1992;Findlay and Dobbs, 1993), consistent with our optical identification. α-OH FAs have been reported in many bacteria, protozoa and yeasts (Zelles et al, 1995). β-OH FAs are associated with the lipopolysaccharides of gram-negative bacteria, but can also be present as the fatty acyl components of phospholipid FAs (Zelles, 1997).…”

Section: Fasmentioning

confidence: 99%

Biogeochemistry of intertidal microbial mats from Qatar: New insights from organic matter characterisation

Słowakiewicz

Whitaker

Thomas

et al. 2016

Organic Geochemistry

View full text Add to dashboard Cite

General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms AbstractUnderstanding early organic matter alteration and preservation in marine carbonate-evaporite systems could improve understanding of carbon cycling and hydrocarbon source rock prediction in such environments. It is possible that organic-rich microbial mats are important contributors to preserved hydrocarbons, and to explore this we examined changes in lipid composition in such a mat from a mesohaline intertidal lagoon, eastern Qatar. The mat reaches > 5 cm thickness over a carbonate mud substrate, rich in seagrass, gastropods and other small bioclasts. Clear lamination, with distinct downward colour changes from green to pink to brown, reflects different microbial mat communities. The layers contain spheroids of probable dolomite, the precipitation of which was plausibly bacterially-mediated. Lipids [n-alkanes, fatty acids (FAs), hopanoids, isoprenoid hydrocarbons, dialkyl glycerol diethers (DAGEs), and isoprenoid (0 to 4) and branched (Ia to IIIa) GDGTs] reflect the diverse mat-building phototrophic, heterotrophic and chemoorganotrophic microorganisms, as well as some likely allochthonous material (i.e. steroids, n-alkanols, high molecular weight nalkanes). The lipids clearly document the change in microbial community, with phytene being the predominant hydrocarbon in the phototrophic surface layer. Oxygen and pH drop significantly 0.2 cm below the mat surface, coincident with the predominance of Deltaproteobacteria and increased concentrations of archaeal and bacterial glycerol dialkyl glycerol tetraether lipids andC 15 /C 16 and C 16 /C 17 dialkyl glycerol ether lipids in the deeper layers. Archaeol, likely of methanogen origin, is most abundant in the deepest layer. Allochthonous inputs occur throughout the mat, including abundant steroids, especially dinosterol and dinostanol, that are possibly related to periodic algal (dinoflagellate) blooms in the Arabian Gulf. Both n-alkanes and n-alkanols appear to be derived from seagrass. Organic matter contents decrease in the deepest mat; this suggests an overall low preservation potential of OM in intertidal mesohaline-hypersaline mats of the Arabian Gulf, which in turn suggests that these are not the major source of hydrocarbons in microbially-dominated carbonateevaporite systems.2

show abstract

Determination of phospholipid- and lipopolysaccharide-derived fatty acids as an estimate of microbial biomass and community structures in soils

Cited by 191 publications

References 21 publications

Chemical Communications

Chemical Communications

Tourist Traffic Significantly Affects Microbial Communities of Sandstone Cave Sediments in the Protected Landscape Area “Labské Pískovce” (Czech Republic): Implications for Regulatory Measures

Biogeochemistry of intertidal microbial mats from Qatar: New insights from organic matter characterisation

Contact Info

Product

Resources

About