Determination of phenolic profiles of Herniaria polygama and Herniaria incana fractions and their in vitro antioxidant and anti-inflammatory effects

Kołodziejczyk-Czepas, Joanna; Kozachok, Solomiia; Pecio, Łukasz; Marchyshyn, S. М.; Oleszek, Wiesław

doi:10.1016/j.phytochem.2021.112861

Cited by 9 publications

(22 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several reports are describing in vitro anti-oxidant activity of Herniaria spp. water-organic and water extracts which were mentioned in our previous paper [15]. As a result, weak (HG, H. hirsuta extracts) or moderate (HIh, H. fontanesii J.…”

Section: Cytotoxicity Assaymentioning

confidence: 74%

“…There is a large amount of evidence indicating that phenolic compounds are closely associated with, and responsible for antioxidant and antiinflammatory activities [13]. Furthermore, in our previous studies, in vitro single isolates from HG such as narcissin, licoagroside B, apiorutin as well as rutin, exhibited moderate antioxidant activity [14], and phenolic fractions from HIh and HP showed profound antioxidant and anti-inflammatory effects [15]. Thus, the detailed phytochemical characterization and quantification of phenolic metabolites in the purified extracts of HG, HP, and HIh were performed using UHPLC-DAD-QTOF-ESI-HRMS (ultra-high-performance liquid chromatography-diode array detector quadrupole time-of-flight electrospray ionisation high-resolution mass spectrometer) and UPLC-DAD-TQD-MS (TQD, tandem triplequadrupole) respectively.…”

Section: Introductionmentioning

confidence: 86%

“…They are suitable for saponin detection, unlike semi-volatile compounds [17]. As a final result, HG-PE, HP-PE, and HIh-PE contained 82%, 62%, and 52% saponins, respectively [14,15].…”

Section: Phytochemical Profiles and The Content Of Individual Phenoli...mentioning

confidence: 96%

“…Commercially available and in-house prepared standards [14,15] were used for the identification and quantification of phenolic compounds in the purified extracts of HG, HP, and HIh. The detailed UV and MS/MS spectra description together with tentative identification of non-triterpenoid constituents in HIh and HP phenolic fractions were presented in our previous work [15]. Due to LC-HRMS analysis 38 Herniaria-derived non-saponin compounds were identified-10 in HG-PE, 17 in HP-PE, and 31 in HIh-PE (Figures 1 and 2 and Table 1).…”

Section: Phytochemical Profiles and The Content Of Individual Phenoli...mentioning

confidence: 99%

See 3 more Smart Citations

Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing Herniaria L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro

et al. 2022

Self Cite

View full text Add to dashboard Cite

The work is aimed at phytochemical characterization and In Vitro evaluation of antioxidant actions, anti-inflammatory effects, and cytotoxicity of purified extracts from three rupturewort (Herniaria L.) species, i.e., Herniaria glabra (HG), H. polygama (HP), and H. incana herb (HIh). The total phenolic content established in the purified extracts (PEs) of HIh, HP, and HG was 29.6, 24.0, and 13.0%, respectively. Thirty-eight non-saponin metabolites were identified using LC-HR-QTOF-ESI-MS; however, only 9 were common for the studied Herniaria species. The most abundant phenolic compound in HG-PE was narcissin (7.4%), HP-PE shared 3 major constituents, namely cis-2-hydroxy-4-methoxycinnamic acid 2-O-β-glucoside (cis-GMCA, 5.8%), narcissin (5.4%), and rutin (5.3%). Almost half of HIh phenolic content (14.7%) belonged to oxytroflavoside A (7-O-methylkaempferol-3-O-[3-hydroxy-3-methylglutaryl-(1→6)]-[α-rhamnopyranosyl-(1®2)]-β-galactopyranoside). Antioxidant properties of the Herniaria PEs were evaluated employing an experimental model of human blood plasma, exposed to the peroxynitrite-induced oxidative stress. The assays demonstrated significant reduction of oxidative damage to protein and lipid plasma components (estimated by measurements of 3-nitrotyrosine, protein thiol groups, thiobarbituric acid-reactive substances), and moderate protection of its non-enzymatic antioxidant capacity. Anti-inflammatory properties of the Herniaria PEs were evaluated In Vitro as inhibitory effects against cyclooxygenases (COX-1 and -2) and concanavalin A-induced inflammatory response of the peripheral blood mononuclear cells (PBMCs). None of the studied plants showed inhibitory effects on COXs but all purified extracts partly reduced the release of interleukin 2 (IL-2) and tumor necrosis factor-alpha (TNF-α) from PBMCs, which suggested their prospective ability to up-regulate inflammatory response of the cells. The purified extract from H. glabra turned out to be the most efficient suppressor of PBMCs’ inflammatory response. Additionally, cytotoxicity of purified Herniaria extracts on PBMCs was ruled out based on In Vitro studies.

show abstract

Section: Cytotoxicity Assaymentioning

confidence: 74%

Section: Introductionmentioning

confidence: 86%

“…They are suitable for saponin detection, unlike semi-volatile compounds [17]. As a final result, HG-PE, HP-PE, and HIh-PE contained 82%, 62%, and 52% saponins, respectively [14,15].…”

Section: Phytochemical Profiles and The Content Of Individual Phenoli...mentioning

confidence: 96%

Section: Phytochemical Profiles and The Content Of Individual Phenoli...mentioning

confidence: 99%

See 2 more Smart Citations

Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing Herniaria L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The content of the chlorogenic acid in the aqueous and ethanolic extracts of Crocus leaves was the same (0.68 mg/g). Cinnamic acid, a precursor in the biosynthesis of flavonoids via the shikimate pathway, was only found in the ethanolic extract of Crocus leaves (1.42 mg/g) [ 27 , 28 ]. Our data indicated that Crocus leaves contained more derivatives of hydroxycinnamic acids and fewer flavonoids.…”

Section: Resultsmentioning

confidence: 99%

Pharmacological Potential and Chemical Composition of Crocus sativus Leaf Extracts

et al. 2021

View full text Add to dashboard Cite

Crocus sativus L. (saffron) has been traditionally used as a food coloring or flavoring agent, but recent research has shown its potent pharmacological activity to tackle several health-related conditions. Crocus sp. leaves, and petals are the by-products of saffron production and are not usually used in the medicine or food industries. The present study was designed to determine the chemical composition of the water and ethanolic extracts of C. sativus leaves and test their cytotoxic activity against melanoma (IGR39) and triple-negative breast cancer (MDA-MB-231) cell lines by MTT assay. We also determined their anti-allergic, anti-inflammatory, and anti-viral activities. HPLC fingerprint analysis showed the presence of 16 compounds, including hydroxycinnamic acids, xanthones, flavonoids, and isoflavonoids, which could contribute to the extracts’ biological activities. For the first time, compounds such as tectoridin, iristectorigenin B, nigricin, and irigenin were identified in Crocus leaf extracts. The results showed that mangiferin (up to 2 mg/g dry weight) and isoorientin (8.5 mg/g dry weight) were the major active ingredients in the leaf extracts. The ethanolic extract reduced the viability of IGR39 and MDA-MB-231 cancer cells with EC50 = 410 ± 100 and 330 ± 40 µg/mL, respectively. It was more active than the aqueous extract. Kaempferol and quercetin were identified as the most active compounds. Our results showed that Crocus leaves contain secondary metabolites with potent cytotoxic and antioxidant activities.

show abstract

Comparative analysis of apocarotenoids and phenolic constituents of Crocus sativus stigmas from 11 countries: Ecological impact

Мykhailenko

Безрук

Ivanauskas

et al. 2022

Archiv der Pharmazie

View full text Add to dashboard Cite

show abstract

Determination of phenolic profiles of Herniaria polygama and Herniaria incana fractions and their in vitro antioxidant and anti-inflammatory effects

Cited by 9 publications

References 84 publications

Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing Herniaria L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro

Comparison of Phenolic Metabolites in Purified Extracts of Three Wild-Growing Herniaria L. Species and Their Antioxidant and Anti-Inflammatory Activities In Vitro

Pharmacological Potential and Chemical Composition of Crocus sativus Leaf Extracts

Comparative analysis of apocarotenoids and phenolic constituents of Crocus sativus stigmas from 11 countries: Ecological impact

Contact Info

Product

Resources

About