Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent

Machado, Bruna Aparecida Souza; Barreto, Gabriele de Abreu; Costa, Aline Silva; Costa, Samantha Serra; Silva, Rejane Pina Dantas; Silva, Danielle Figuerêdo da; Brandão, Hugo Neves; Rocha, João; Nunes, Silmar Baptista; Umsza-Guez, Marcelo Andrés; Padilha, Francine Ferreira

doi:10.1371/journal.pone.0134489

Cited by 48 publications

(56 citation statements)

References 93 publications

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“…Ethanol is a widely used solvent for extracting biologically active compounds from propolis [41]. A previous study comparing the extraction methods used for propolis had concluded that ethanol extracts have the maximum anti-bacterial effect, which can be attributed to their highest content of phenolics and flavonoids [42], which validates our findings that Eth extract is more effective than EA extract in terms of anti-bacterial effect.…”

Section: Discussionsupporting

confidence: 88%

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

et al. 2017

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Propolis obtained from bee hives is a natural substance with antimicrobial properties. It is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of Malaysian propolis were prepared. Both the extracts displayed antimicrobial and anti-biofilm properties against Enterococcus faecalis, a common bacterium associated with hospital-acquired infections. High performance liquid chromatography (HPLC) analysis of propolis revealed the presence of flavonoids like kaempferol and pinocembrin. This study investigated the role of propolis developed into nanoparticles with chitosan for its antimicrobial and anti-biofilm properties against E. faecalis. Bacteria that grow in a slimy layer of biofilm are resistant to penetration by antibacterial agents. The use of nanoparticles in medicine has received attention recently due to better bioavailability, enhanced penetrative capacity and improved efficacy. A chitosan-propolis nanoformulation was chosen based on ideal physicochemical properties such as particle size, zeta potential, polydispersity index, encapsulation efficiency and the rate of release of the active ingredients. This formulation inhibited E. faecalis biofilm formation and reduced the number of bacteria in the biofilm by ~90% at 200 μg/ml concentration. When tested on pre-formed biofilms, the formulation reduced bacterial number in the biofilm by ~40% and ~75% at 200 and 300 μg/ml, respectively. The formulation not only reduced bacterial numbers, but also physically disrupted the biofilm structure as observed by scanning electron microscopy. Treatment of biofilms with chitosan-propolis nanoparticles altered the expression of biofilm-associated genes in E. faecalis. The results of this study revealed that chitosan-propolis nanoformulation can be deemed as a potential anti-biofilm agent in resisting infections involving biofilm formation like chronic wounds and surgical site infections.

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Section: Discussionsupporting

confidence: 88%

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

et al. 2017

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“…The conventional technique uses ethanol as solvent, while alternative methods, such as extraction of supercritical fluids, have also been described. The extraction method directly affects the yield and selectivity of some components (Machado et al, 2015, Machado et al, 2016. Importantly, propolis obtained by aqueous extraction contains amino acids, organic matter and carbohydrates, which are not present in the ethanol extraction.…”

Section: Factors Influencing the Red Propolis Compositionmentioning

confidence: 99%

Chemical characterization and pharmacological action of Brazilian red propolis

et al. 2018

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Among natural products, Brazilian propolis stands out for being considered a source for the discovery of new bioactive compounds. In the last decade, a new Brazilian propolis has been described, known as red propolis due to its reddish pigment. Since then, numerous studies revealing its major components and pharmacological activity have been carried out. Thus, this research had the aim of performing an integrative review of literature on the biological activity of Brazilian red propolis. Scientific studies in the PubMed, SciELO and LILACS databases were reviewed and analyzed using an inductive approach and indirect documentation technique. A total of 418 publications were found, and inclusion and exclusion criteria were subsequently applied, selecting a total of 18 articles. Brazilian red propolis presents numerous pharmacological properties, especially antibacterial, antifungal, antioxidant and anti-inflammatory effects. In addition, the chemical composition of this substance is different from other types of propolis already reported in literature, presenting flavonoids never before described. In this way, its chemical characteristics suggest probable therapeutic use for human consumption Keywords: Flavonoids, Apis millifera, bee. Caracterização química e ação farmacológica da própolis vermelha brasileira ResumoDentre os produtos naturais, a própolis brasileira destaca-se por ser considerada uma fonte para a descoberta de novos compostos bioativos. Na última década, foi descrita uma nova própolis brasileira, conhecida como própolis vermelha devido ao seu pigmento avermelhado. Desde então, inúmeras pesquisas revelando seus componentes majoritários e sua atividade farmacológica têm sido descritas. Dessa forma, esta pesquisa teve o objetivo de realizar uma revisão integrativa da literatura sobre a atividade biológica da própolis vermelha brasileira. Estudos científicos nos bancos de dados PubMed, SciELO e LILACS foram revisados e analisados utilizando-se uma abordagem indutiva e técnica de documentação indireta. Foram encontradas 418 publicações, sendo posteriormente aplicados critérios de inclusão e exclusão, selecionando ao todo 18 artigos. A própolis vermelha brasileira apresenta inúmeras propriedades farmacológicas, destacando-se os efeitos antibacteriano, antifúngico, antioxidante e anti-inflamatório. Além disso, a composição química desta substância é diferente dos demais tipos de própolis já relatados na literatura, apresentando flavonóides que ainda não tinham sido descritos. Dessa forma, suas características químicas sugerem provável uso terapêutico para o organismo humano.

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“…The critical temperature is the highest one, at which the gas can be converted in liquid through the increase in pressure. The critical pressure is the highest one, at which the liquid can be converted in gas through the increase in the liquid's temperature (Gomez and Ossa, 2002;Machado et al, 2015;Machado et al, 2016a).…”

Section: Supercritical Extractionmentioning

confidence: 99%

Phytochemical importance and utilization potential of grape residue from wine production

Cirqueira¹,

Costa²,

Barbosa³

et al. 2017

Afr. J. Biotechnol.

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Grapes are placed at a distinct position, both in the economy and at nutritional and phytochemical levels. The number of studies performed aimed at identifying the various bioactive compounds of grapes, how their cellular structures are linked, efficient methods of extraction and its effect on human health are rather relevant. The use of grapes for wine production is a worldwide activity, therefore a great quantity of residues are generated. These, in turn, are commonly used in soil fertilization and animal feed. Research in the food, pharmaceutical, medical and agricultural industries have revealed a great potential of using grape residue to recuperate phenolic compounds, flavonoids and especially the resveratrol. These have been found by these researches to offer benefits associated to its consumption, such as improvements in glucose tolerance in diabetic patients, decrease in the occurrence of cardiovascular diseases, diminishing the symptoms of menopause, protection against osteoporosis, cancer and Alzheimer's disease. The present work aimed at gathering information about the characterization of the phytochemical content of grapes and its residues, as well as gathering data about the extraction process of such compounds and their application in the food industry.

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Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent

Cited by 48 publications

References 93 publications

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

Chemical characterization and pharmacological action of Brazilian red propolis

Phytochemical importance and utilization potential of grape residue from wine production

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