Determination of organophosphate pesticides at a carbon nanotube/organophosphorus hydrolase electrochemical biosensor

Deo, Randhir P.; Wang, Joseph; Block, Ines; Mulchandani, Ashok; Joshi, Kanchan A.; Trojanowicz, Marek; Scholz, Fritz; Chen, Wilfred; Lin, Yuehe

doi:10.1016/j.aca.2004.09.072

Cited by 245 publications

(100 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4, the fluorescent quenching efficiency of furazolidone was linear within the range of 2-160 mM, which was comparable to other earlier reported biosensor (Kumar and D'Souza, 2010). The LOD of the fluorescent biosensor was 0.5 mM [signal-to-noise (S/N) ratio ¼3], which was also comparable to a carbon nanotube/organophosphorus hydrolase electrochemical biosensor (Deo et al, 2005) and amperometric microbial biosensor for direct determination of organophosphate pesticides (Mulchandani et al, 2001). …”

Section: Sensitivity Of Furazolidone Detection By Fluorescent Biosensorsupporting

confidence: 82%

Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone

Yin

Zhang

Chen

2014

Biosensors and Bioelectronics

View full text Add to dashboard Cite

a b s t r a c tMethods for the rapid and sensitive detection of furazolidone, a pesticide used for the treatment of infections of animals and human beings, have been urgently recommended for its large residual, strong carcinogenicity and genotoxicity in the environment. In this study, a method for the detection of furazolidone based on the rapid fluorescence quenching of pyoverdine by furazolidone was developed. Pyoverdine secreted by a Pseudomonas aeruginosa strain PA1 was purified through affinity chromatography and its fluorescent property was characterized. The fluorescence of pyoverdine could be quenched by furazolidone with specificity, and based on this phenomenon a fluorescent method for furazolidone detection was established. Fluorescence of pyoverdine was quenched by furazolidone probably due to the electron transfer from pyoverdine to furazolidone. The optimal pH for the detection was 7.2 in 50 mM 3-(N-Morpholino) propanesulfonic acid solution, and the whole detection process could be completed within a few seconds. The linear range of the detection was 2-160 mM and the limit of detection (LOD) was 0.5 mM. This study developed a novel fluorescent method for furazolidone detection, and the rapid and specific fluorescent biosensor can be potentially applied for furazolidone detection in the aquatic samples.

show abstract

Section: Sensitivity Of Furazolidone Detection By Fluorescent Biosensorsupporting

confidence: 82%

Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone

Yin

Zhang

Chen

2014

Biosensors and Bioelectronics

View full text Add to dashboard Cite

show abstract

“…Enzyme/CNT-based biosensors have been used to detect not only small biomolecules but also environmental pollutants (e.g., organophosphate (OP) pesticides [32,33]). The mechanism of this biosensor for detecting OPs is based on inhibiting enzyme activity by these toxic chemicals.…”

Section: Enzyme-cnt Biosensors-mostmentioning

confidence: 99%

“…Very recently, Deo and co-workers reported an amperometric biosensor for OP pesticides based on a CNT-modified transducer and an organophosphorus hydrolase (OPH) biocatalyst [32]. A bilayer approach with the OPH layer at the top of the CNT film was used to prepare the CNT/OPH biosensor.…”

Section: Enzyme-cnt Biosensors-mostmentioning

confidence: 99%

“…The CNT layer leads to a greatly improved anodic detection of the enzymatically-generated p-nitrophenol product, including higher sensitivity and stability. Under optimal conditions, the biosensor was used to measure levels as low as 0.15 mM paraoxon and 0.8 mM methyl parathion with sensitivities of 25 nA/mLM and 6 nA/mLM, respectively [32].…”

Section: Enzyme-cnt Biosensors-mostmentioning

confidence: 99%

See 1 more Smart Citation

Functionalized carbon nanotubes and nanofibers for biosensing applications

Wang

Lin

2008

TrAC Trends in Analytical Chemistry

Self Cite

261

130

View full text Add to dashboard Cite

This review summarizes recent advances in electrochemical biosensors based on carbon nanotubes (CNTs) and carbon nanofibers (CNFs) with an emphasis on applications of CNTs. CNTs and CNFs have unique electric, electrocatalytic and mechanical properties, which make them efficient materials for developing electrochemical biosensors.We discuss functionalizing CNTs for biosensors. We review electrochemical biosensors based on CNTs and their various applications (e.g., measurement of small biological molecules and environmental pollutants, detection of DNA, and immunosensing of disease biomarkers). Moreover, we outline the development of electrochemical biosensors based on CNFs and their applications. Finally, we discuss some future applications of CNTs.

show abstract

“…Single-walled carbon nanotube (SWCNT) chemiresistors and chemical field effect transistors (Chem-FET) have been shown to provide suitable platforms for the detection of various gases. [10][11][12][13][14][15] Random networks of functionalized SWCNTs have produced sensors that are inexpensive to fabricate, operate at room temperature, and have ultra-low power requirements. [16,17] Theoretical and experimental reports have suggested that CO does not engage in charge transfer with pristine SWCNTs, [18][19][20] indicating the a chemical reactive interface is necessary.…”

mentioning

confidence: 99%

Bio‐Inspired Carbon Monoxide Sensors with Voltage‐Activated Sensitivity

Savagatrup

Schroeder

et al. 2017

Angewandte Chemie

View full text Add to dashboard Cite

Correspondence to: Timothy M. Swager. + These authors contributed equally to this work.Supporting information and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.org/ 10.1002/anie.201707491. Conflict of interestThe authors declare the following competing financial interest(s): A patent has been filed on this technology. HHS Public Access Author Manuscript Author ManuscriptAuthor Manuscript Author ManuscriptCarbon monoxide (CO) outcompetes oxygen when binding to the iron center of hemeproteins, leading to a reduction in blood oxygen level and acute poisoning. Harvesting the strong specific interaction between CO and the iron porphyrin provides a highly selective and customizable sensor. We report the development of chemiresistive sensors with voltage-activated sensitivity for the detection of CO comprising iron porphyrin and functionalized single-walled carbon nanotubes (F-SWCNTs). Modulation of the gate voltage offers a predicted extra dimension for sensing. Specifically, the sensors show significant increase in sensitivity toward CO when negative gate voltage is applied. The dosimetric sensors are selective to ppm levels of CO and functional in air. UV/Vis spectroscopy, differential pulse voltammetry, and density functional theory reveal that the in situ reduction of Fe III to Fe II enhances the interaction between the F-SWCNTs and CO. Our results illustrate a new mode of sensors wherein redox active recognition units are voltageactivated to give enhanced and highly specific responses. CommunicationsRevving up for sensing: A platform for voltage-activated, chemiresistive gas detection has been delveloped based on covalently functionalized single-walled carbon nanotubes decorated with voltage-responsive chemical selectors. The sensor is highly selective towards CO in the presence of oxygen, nitrogen, and carbon dioxide and is robust to humidity and remains operational in air. KeywordsCarbon monoxide; carbon nanotubes; iron porphyrin; sensors; voltage-activated Carbon monoxide (CO) is responsible for more than half of all fatal poisoning worldwide. [1] Exposure to the colorless, tasteless, and odorless gas is difficult to discern as the initial symptoms of poisoning (headache, dizziness, and confusion) are nonspecific. In the United States, the Occupational Safety and Health Administration (OSHA) has designated permissible exposure limits of 50 ppm over eight hours and 200 ppm over five minutes. [2] The affinity of iron porphyrin towards CO is well-documented for the enzymes cytochrome P450, [3][4][5] hemoglobin, [6] and myoglobin. [7] This high affinity for CO over O 2 of hemoglobin and myoglobin is the underlying mechanism of carbon monoxide poisoning in mammals. [8,9] Although detectors for CO are available, there remains a need for massively [16,17] Theoretical and experimental reports have suggested that CO does not engage in charge transfer with pristine SWCNTs, [18][19][20] indicating the a chemical reactive interface is necessary. Conductivity...

show abstract

Determination of organophosphate pesticides at a carbon nanotube/organophosphorus hydrolase electrochemical biosensor

Cited by 245 publications

References 22 publications

Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone

Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone

Functionalized carbon nanotubes and nanofibers for biosensing applications

Bio‐Inspired Carbon Monoxide Sensors with Voltage‐Activated Sensitivity

Contact Info

Product

Resources

About