Determination of Nonylphenol in a Highly Sensitive Chemiluminescent Immunoenzyme Assay of Natural Waters

Abstract

A competitive chemiluminescent immunoenzyme assay (CL-EIA) technique is proposed for the sensitive determination of one of the environmentally significant toxicants of anthropogenic origin-nonylphenol—in natural waters. The chosen chemiluminescent detection is characterized by a higher sensitivity compared to the colorimetric. The limit of nonylphenol detection was 9 ng/mL compared to 55 ng/mL for colorimetric one in optimal conditions. The developed analysis can be used for two purposes; it is highly sensitive for the possibility of toxicological analysis and dilution of complex matrices with raw buffer solution, as well as for the analysis of water samples without pretreatment and dilution. The method has a working range from 28 to 1800 ng/mL. The degree of nonylphenol revealing in the spiked samples of river, spring, and waterfall water was 82–119%.