Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC

Koppes, Sjors A.; Kemperman, P. M. J. H.; Tilburg, I. Van; Calkoen-Kwa, F.; Engebretsen, Kristiane A.; Puppels, Gerwin J.; Kežić, Sanja

doi:10.1080/1354750x.2016.1256428

Cited by 27 publications

(41 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For left cheek zone (Figure B), increased intensities are seen in bands of 1275 and 1454 cm −1 . These bands are related to different proteins, being the band of 1454 cm −1 the one that is correlated to Raman spectra of collagen reported in previous works …”

Section: Resultssupporting

confidence: 67%

Use of Raman spectroscopy in the assessment of skin after CO₂ablative fractional laser surgery on acne scars

et al. 2019

View full text Add to dashboard Cite

Acne is a skin disease with chronic and inflammatory characteristics that is present on the pilosebaceous unit appearing first at puberty and often in adulthood. Its pathophysiology involves hyperseborrhea, non-normal follicular-based keratinization, and Propionibacterium acnes increase in the pilosebaceous unit resulting in a change in the cutaneous microenvironment leading to inflammatory reactions. 1 Ablative fractional laser (AFL) surgery is widely used in the medical field because it is able to reduce trauma due to the short-time exposure to radiation. 2 Isotretinoin has been considered for several decades the gold standard for nodulocystic acne or inflammatory acne, which has failed other course treatments, due to its anti-inflammatory properties. 3It has been established that combining the use of AFL surgery with oral isotretinoin improvement is observed on acne scars in a short period of time with no acne relapse or adverse effects over the resurfaced skin. 4-8 Abstract Background: Ablative fractional laser surgery is a common technique for treating acne scars. However, an in vivo and noninvasive analysis of the histologic variations between acne skin and the resulting resurfaced skin is needed in order to evaluate the wound healing process of the scars induced by the ablative fractional laser surgery. Materials and Methods: Nine patients with acne scars underwent a single treatment with a CO 2 ablative fractional laser surgery. Collagen presence on the resurfaced skin was noninvasively assessed by means of Raman spectroscopy and principal component analysis.Results: Principal component analysis shows that all the patients presented a collagen regeneration on the resurfaced skin after the laser treatment. Conclusion:Collagen plays a crucial role in the wound healing process. By assessing the collagen presence on the skin, it was possible to quantify the regenerative effects of the ablative fractional laser in a noninvasive way.

show abstract

Section: Resultssupporting

confidence: 67%

Use of Raman spectroscopy in the assessment of skin after CO₂ablative fractional laser surgery on acne scars

et al. 2019

View full text Add to dashboard Cite

show abstract

“…30% of the SC depth [16], determining the highest skin barrier function at this depth. These results correlate with further parameters sensitive to the skin barrier function that were determined using in vivo CRM, such as the NMF concentration [17], or the hydrogen bonding state of water [18]. One perception from these studies was, that the maximal skin barrier function occurs at the depths 20-40% of the SC thickness.…”

supporting

confidence: 58%

Non-invasive depth profiling of the stratum corneum in vivo using confocal Raman microscopy considering the non-homogeneous distribution of keratin

Darvin

Choe

Schleusener

et al. 2019

Biomed. Opt. Express

View full text Add to dashboard Cite

Confocal Raman microscopy has a number of advantages in investigating the human stratum corneum (SC) in vivo and ex vivo. The penetration profiles of xenobiotics in the SC, as well as depth profiles of the physiological parameters of the SC, such as the concentration of water depending on the strength of hydrogen bonds, total water concentration, the hydrogen bonding state of water molecules, concentration of intercellular lipids, the lamellar and lateral packing order of intercellular lipids, the concentration of natural moisturizing factor molecules, carotenoids, and the secondary and tertiary structure properties of keratin are well investigated. To consider the depth-dependent Raman signal attenuation, in most cases a normalization procedure is needed, which uses the main SC's protein keratin-related Raman peaks, based on the assumption that keratin is homogeneously distributed in the SC. We found that this assumption is not accurate for the bottom part of the SC, where the water concentration is considerably increased, thus, reducing the presence of keratin. Our results demonstrate that the bottom part of the SC depth profile should be multiplied by 0.94 in average in order to match this non-homogeneity, which result in a decrease of the uncorrected values in these depths. The correctly normalized depth profiles of the concentration of lipids, water, natural moisturizing factor and carotenoids are presented in this work. The obtained results should be taken into consideration in future skin research using confocal Raman microscopy. IntroductionThe stratum corneum (SC), the outermost continuously renewing skin layer, provides the barrier function. It is important for the regulation of water balance and the penetration ability of xenobiotics through the skin. The SC consists of corneocytes (nuclear-free and cytoplasmic organelles-free flattened cells), embedded in the membrane-like ordered intercellular lipid matrix. The corneocytes, which consist of keratin filaments, natural moisturizing factor (NMF) molecules and water, are surrounded by a cornified envelope representing a hardpermeable tough protein/lipid polymer structure [1]. The intercellular lipids (ICL) of the lipid matrix are organized in the form of a classical membrane and their orthorhombic lateral organization represents a highly ordered and very densely packed structure, which is notpermeable for the majority of the non-destructive substances [2][3][4]. The depth distribution of the lateral organization of ICL is non-homogeneous in the SC, showing a prevalence of orthorhombic over hexagonal ordering in the intermediate SC depths measured in vitro [5,6]. The prevalence of the orthorhombic lateral organization in the SC maintains the skin barrier

show abstract

“…38,39 This results in characteristic Raman spectra, which are noninvasive molecular measurements of filaggrin degradation products within the SC. 40 Koppes et al 41 compared both RMS and HPLC. They showed a strong correlation between the 2 methods, despite the methods measuring different NMF components.…”

Section: Measuring Filaggrin Protein Expression In Vivomentioning

confidence: 99%

“…However, unlike RMS, it requires further processing for HPLC analysis, prolonging the time to results. 41 O'Regan et al 13 looked at the relationship between Raman NMF signatures of the stratum corneum and FLG mutation status in patients with moderate-to-severe atopic dermatitis.…”

Section: Measuring Filaggrin Protein Expression In Vivomentioning

confidence: 99%

The role of filaggrin in atopic dermatitis and allergic disease

Drislane

Irvine

2020

Annals of Allergy, Asthma & Immunology

212

178

View full text Add to dashboard Cite

Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC

Abstract: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.

Cited by 27 publications

References 24 publications

Use of Raman spectroscopy in the assessment of skin after CO₂ablative fractional laser surgery on acne scars

Use of Raman spectroscopy in the assessment of skin after CO₂ablative fractional laser surgery on acne scars

Non-invasive depth profiling of the stratum corneum in vivo using confocal Raman microscopy considering the non-homogeneous distribution of keratin

The role of filaggrin in atopic dermatitis and allergic disease

Contact Info

Product

Resources

About

Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC

Abstract: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.

Cited by 27 publications

References 24 publications

Use of Raman spectroscopy in the assessment of skin after CO2ablative fractional laser surgery on acne scars

Use of Raman spectroscopy in the assessment of skin after CO2ablative fractional laser surgery on acne scars

Non-invasive depth profiling of the stratum corneum in vivo using confocal Raman microscopy considering the non-homogeneous distribution of keratin

The role of filaggrin in atopic dermatitis and allergic disease

Contact Info

Product

Resources

About

Use of Raman spectroscopy in the assessment of skin after CO₂ablative fractional laser surgery on acne scars

Use of Raman spectroscopy in the assessment of skin after CO₂ablative fractional laser surgery on acne scars