Determination of N ε -carboxymethyllysine in heated milk products by immunochemical methods

In Vitro Formation of Nϵ-(Carboxymethyl)lysine and Imidazolones under Conditions Similar to Continuous Ambulatory Peritoneal Dialysis

“…The assay was performed as described in literature (17) with the exception that the plates were coated with 1 g/ml CML-ovalbumin. For competitive antibody binding, 500 l of each sample were mixed with 500 l of a 1:12500 dilution of polyclonal CML-antiserum in PBS containing 0.4% BSA and 0.1% Tween 20.…”

Comparative LC–MS/MS profiling of free and protein-bound early and advanced glycation-induced lysine modifications in dairy products

“…At present, a plethora of different analytical approaches have been employed to determine glycation products in milk and dairy products. CML, for instance, was quantified by ELISA in infant formulas [12], heated milk and milk powder [13] and human breast milk [14], by using a chromatographic technique, e.g. GC/MS [15,16], HPLC/FD [17,18] or by LC-MS/MS [19,20] for CML analyses in raw, pasteurized and UHT milk, as well as in infant formulas.…”

Simultaneous analysis of lysine, Nɛ-carboxymethyllysine and lysinoalanine from proteins

“…Moreover, other oxidative pathways such as lipid peroxidation and serine oxidation may also be involved [8][9][10]. CML formation can be estimated in food or protein model system mainly through gas chromatography (GC) [9,[11][12][13][14], high performance liquid chromatography (HPLC) [15][16][17][18][19], matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) [20,21,22] and enzyme-linked immunosorbent assay (ELISA) [5,[23][24][25][26][27].…”