Determination of mycotoxins in pomegranate fruits and juices using a QuEChERS-based method

Myresiotis, Charalampos K.; Testempasis, Stefanos; Vryzas, Zisis; Karaoglanidis, G. S.; Papadopoulou‐Mourkidou, Euphemia

doi:10.1016/j.foodchem.2015.02.141

Cited by 80 publications

(48 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, dichloromethane is toxic and is not recommended. The sorbent GCB was not suitable for the detection of mycotoxins in pomegranate juices [17] and beer-based drinks [2] due to the sorption of the mycotoxins, so we may not choose GCB as an alternative cleaning sorbent.…”

Section: Resultsmentioning

confidence: 99%

“…The successful application of this method has been reported in many products, including cereals and their products [16], spices [17], nuts and seeds [18], Madeira wine [19], human breast milk [20], sesame butter [21], noodles [22], eggs [23], human biological fluids [24], and baby food [25]. In order to reduce the matrix effects (MEs), which can result in unsatisfactory recoveries, additional purification with sorbents such as octadecyl silica (C18), primary secondary amine (PSA), and graphitized carbon black (GCB) are commonly used.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

QuEChERS Purification Combined with Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry for Simultaneous Quantification of 25 Mycotoxins in Cereals

Sun

Zhang

et al. 2016

Toxins

View full text Add to dashboard Cite

A method based on the QuEChERS (quick, easy, cheap, effective, rugged, and safe) purification combined with ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS), was optimized for the simultaneous quantification of 25 mycotoxins in cereals. Samples were extracted with a solution containing 80% acetonitrile and 0.1% formic acid, and purified with QuEChERS before being separated by a C18 column. The mass spectrometry was conducted by using positive electrospray ionization (ESI+) and multiple reaction monitoring (MRM) models. The method gave good linear relations with regression coefficients ranging from 0.9950 to 0.9999. The detection limits ranged from 0.03 to 15.0 µg·kg−1, and the average recovery at three different concentrations ranged from 60.2% to 115.8%, with relative standard deviations (RSD%) varying from 0.7% to 19.6% for the 25 mycotoxins. The method is simple, rapid, accurate, and an improvement compared with the existing methods published so far.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

QuEChERS Purification Combined with Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry for Simultaneous Quantification of 25 Mycotoxins in Cereals

Sun

Zhang

et al. 2016

Toxins

View full text Add to dashboard Cite

show abstract

“…The QuEChERS method is well known for its applicability in the simultaneous analysis of a large number of pesticides in a variety of food matrices with a water content of more than 80%. [24][25] Conversely, Hedyotis diffusa and Semifluid extract of deer feotus are special products with water content < 10%; they cannot be used in the QuEChERS extraction directly. Thus, for them, a certain proportion of water was added during the extraction procedure in order to improve the water content and to achieve a good extraction efficiency.…”

Section: Optimization Of Extraction Methodsmentioning

confidence: 99%

Multi-residue Method for Determination of Selected Neonicotinoid Insecticides in Traditional Chinese Medicine Using Modified Dispersive Solid-phase Extraction Combined with Ultra-performance Liquid Chromatography Tandem Mass Spectrometry

Qin¹,

Chen

Yang

et al. 2015

ANAL. SCI.

View full text Add to dashboard Cite

A reliable and cost-effective method for the determination of multiple neonicotinoids was developed using a modified QuEChERS-based extraction procedure in complex matrices, namely Hedyotis diffusa (a representative of the Traditional Chinese herb which contains lots of pigment, saponin and terpene) and Semifluid extract of deer foetus (a representative of the Chinese traditional patent medicine that was produced with several different herbs, and especially containing lots of protein, except for other interference components). Ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for the quantification and confirmation of five compounds. Except for two transitions obtained by the MRM mode, identification was further carried out by the ion radios. The proposed chemical structure of every selected product ion and the proposed pyrolysis way were presented. The extraction, clean-up, UPLC separation and MS/MS parameters were especially optimized in order to obtain better recoveries. The low limits of detection (LODs) of five insecticides ranged from 0.04 to 0.81 μg kg -1 . Matrix matched calibration in the concentration range of 0.05 -50 μg kg -1 were used to compensate the matrix effect, and reasonable recoveries 80.2 -105.4% of five compounds were demonstrated in different spiked levels with inter-RSD from 1.7 to 10.6%. The proposed method is an alternative approach to make an analysis of neonicotinoids in Chinese medicine, which is more reliable and promising compared with other detection methods.

show abstract

“…The use of clean-up becomes an important tool to prolong the equipment useful life, besides reduce the matrix effects when complex matrices, such as wine, are injected. 18 In the last years, dispersive solid phase extraction (d-SPE) have been successfully used as clean-up technique in a large variety of matrices and analytes [19][20][21][22][23] including wine samples. [24][25][26] Besides, performing the extract dilution prior injection into ultrahigh performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) system has proven to be a non-expensive alternative to reduce matrix effect and increase sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Fast Sample Preparation Method Using Ultra-High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Natamycin Determination in Wine Samples

Bernardi¹,

Rizzetti²,

Adaime³

et al. 2016

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

An easy and efficient sample preparation method was developed for the determination of natamycin residues in wine samples by solvent extraction and ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) analysis. After choosing acetonitrile as extraction solvent, an experimental design was carried out with different amounts of C18 and primary secondary amine (PSA) sorbents in order to optimize the clean-up step. Validation results were satisfactory, with recoveries from 77 to 95% and relative standard deviation (RSD) lower than 10% for the spike levels 5, 10 and 20 µg L , respectively. The validated method proved to be an excellent analytical tool, with a total analysis time of 3 min. The method was applied in 10 Brazilian wine samples and no residues of natamycin were detected. Keywords: natamycin, wine, sample preparation, UHPLC-MS/MS IntroductionThe use of preservatives in food and beverages is necessary to prevent or retard microbial growth avoiding undesirable alterations in the final product quality. Among the antimicrobial substances employed by the food industry, natamycin has been proven an effective alternative for controlling microbial growth.1 Natamycin or pimaricin is an antibiotic belonging to the group of polyene macrolides produced by actinomycetes Streptomyces natalensis. 2,3 Several studies reported the use of natamycin for the surface treatment of cheeses and dry sausages [4][5][6] and most recently, in antimicrobial packaging systems. 7 Natamycin cannot be added to wines because it is classified as a non-permitted additive by the Code of Enological Practices of the International Organization of Vine and Wine (OIV). ) where it is used as an alternative to sulfur dioxide during bottling, preventing refermentation in sweet and low acidity wines. 9 However, export wines must comply with EU standards and currently a level of 5 µg L -1 is enforced in countries as Germany. 10 Recently, the European disease control authorities detected natamycin in wine samples from different producing countries and banned their sale and distribution. 11,12 Besides addition to the wine, many wineries use natamycin on the walls and floors to eliminate the yeast and mold in the environment. Under these conditions, natamycin can be transferred into the wine being a contamination source.In Brazil, the Brazilian Health Regulatory Agency (ANVISA) did not authorize the use of natamycin in wines, only on cheese surface with a maximum limit of 2 mg per 100 cm 2 and meat surface of embedded products with a maximum limit of 1 mg per dm 2 . 13 However, even the use of this not authorized compound, in the last years several wine samples were confiscated from Brazilian wineries due to the use of natamycin.14 Generally, the analytical methods for natamycin determination in food and beverages samples use organic solvents for extraction followed by filtration and determination by liquid chromatography. Roberts et al. 15 developed a rapid procedure based on liquid chromatography coupled to hig...

show abstract

Determination of mycotoxins in pomegranate fruits and juices using a QuEChERS-based method

Cited by 80 publications

References 33 publications

QuEChERS Purification Combined with Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry for Simultaneous Quantification of 25 Mycotoxins in Cereals

QuEChERS Purification Combined with Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry for Simultaneous Quantification of 25 Mycotoxins in Cereals

Multi-residue Method for Determination of Selected Neonicotinoid Insecticides in Traditional Chinese Medicine Using Modified Dispersive Solid-phase Extraction Combined with Ultra-performance Liquid Chromatography Tandem Mass Spectrometry

Fast Sample Preparation Method Using Ultra-High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Natamycin Determination in Wine Samples

Contact Info

Product

Resources

About