Determination of muscle relaxants pancuronium and vecuronium bromide by capillary electrophoresis with capacitively coupled contactless conductivity detection

Abstract

A novel capillary electrophoretic method for the separation of pancuronium (PM) and vecuronium (VM) ions utilizing capacitively coupled contactless conductivity detection was devised and validated. The separation was carried out in bare fused-silica capillaries (50 μm id, 75/45 cm) at 25°C. Optimal BGE was 50 mM borate buffer of pH 9.5 containing 12.5 mg/mL of (2-hydoxypropyl)-γ-CD. The samples were injected hydrodynamically at 1000 mbar for 3 s. Separation was performed at +30 kV. Under such conditions the PM and VM were base-line resolved and the separation took < 4 min. For quantification phenyltrimethylammonium iodide was used as internal standard. Calibration curves were linear for both pancuronium bromide (PMB) and vecuronium bromide (VMB) in the range 25-250 μg/mL with r> 0.9968. The limits of detection were 7 and 6 μg/mL for PMB and VMB, respectively. The accuracy tested by recovery experiment at three concentration levels of added PMB and VMB was satisfactory (95.7-102.7%, n =3, with RSD < 2.61%). The method was successfully applied to the assay of PMB and VMB in commercial injection solutions.

Contactless conductivity detection for analytical techniques: Developments from 2010 to 2012

“…Aspirin and paracetamol, two of the three worldwide mostly used pain killers, were determined in pharmaceutical powders with a modified C 4 D detector using contactless electromagnetic induction . Other examples of CE‐C 4 D determination of non‐UV‐absorbing species in pharmaceutical preparations cover the analysis of antimycotic drugs , muscle relaxants , antiseptic/antimicrobial preparation , and manitol . Dual opposite end injection, which enables simultaneous CE analysis of cations and anions, was applied to the determination of basic drugs and their counter anions in various pharmaceutical formulations .…”

Recent advances in applications of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE‐C4D): an update

“…Petrů et al . () have introduced a novel CE‐C 4 D method for of the assay of pancuronium (PM) and vecuronium (VM) ions in injection solution. The separation was carried out in BGE composed of 50 m m borate buffer of pH 9.5 containing 12.5 mg/mL of (2‐hydoxypropyl)‐ γ ‐cyclodextrin (CD).…”

“…The results obtained using CE-C 4 D were compared with the results obtained by the recommended methods of the United States Pharmacopeia (2009) potentiometric titration and spectrophotometry and were found to have better selectivity. Petrů et al (2011a) have introduced a novel CE-C 4 D method for of the assay of pancuronium (PM) and vecuronium (VM) ions in injection solution. The separation was carried out in BGE composed of 50 mM borate buffer of pH 9.5 containing 12.5 mg/mL of (2-hydoxypropyl)-g-cyclodextrin (CD).…”

Rapid determination of branched chain amino acids in human blood plasma by pressure‐assisted capillary electrophoresis with contactless conductivity detection

“…The use of C 4 D has great advantages in the CE determination of AAs and other non‐absorbing substances, such as saccharides , biogenic amines , organic acids , drugs , etc. Electrochemical detection based on the principle of C 4 D is a universal detection technique that does not necessitate derivatisation of biogenic substances and these substances can be detected in their native form .…”