Determination of microphytobenthos PSII quantum efficiency and photosynthetic activity by means of variable chlorophyll fluorescence

Kromkamp, Jacco C.; Barranguet, Christiane; Peene, J.

doi:10.3354/meps162045

Cited by 204 publications

(203 citation statements)

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“…This result fits well with the ecology of these growth forms. EPL is not only more abundant in muddy cohesive sediments where light penetration is more restricted than in sandy sediments (Paterson and Hagerthey 2001;Cartaxana et al, 2011), but, more importantly, their (micro-) migratory behaviour allows positioning at the optimal irradiance in the vertical light gradient and rapid escape from periodic excess light (Kromkamp et al, 1998;Conn et al, 2004;Consalvey et al, 2004;Serô dio et al, 2006). This alleviates the need to invest in a strong physiological capacity to respond to light stress as previously proposed Cartaxana et al, 2011), although the right balance between motility and physiology still remains essential (van Leeuwe et al, 2009;Perkins et al, 2010b;Cartaxana et al, 2011;Serô dio et al, 2012).…”

Section: Photoprotection In Intertidal Benthic Diatomsmentioning

confidence: 76%

“…Behavioural photoprotection involves motility, allowing cells to position themselves in light gradients and escape from prolonged exposure to excess light (Admiraal, 1984;Kromkamp et al, 1998;Consalvey et al, 2004;Serô dio et al, 2006). In addition, both motile and non-motile species use fast regulatory physiological processes for photoprotection (that is, 'physiological photoprotection'; Lavaud, 2007;Goss and Jakob, 2010;Depauw et al, 2012;Lepetit et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Growth form defines physiological photoprotective capacity in intertidal benthic diatoms

et al. 2014

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In intertidal marine sediments, characterized by rapidly fluctuating and often extreme light conditions, primary production is frequently dominated by diatoms. We performed a comparative analysis of photophysiological traits in 15 marine benthic diatom species belonging to the four major morphological growth forms (epipelon (EPL), motile epipsammon (EPM-M) and non-motile epipsammon (EPM-NM) and tychoplankton (TYCHO)) found in these sediments. Our analyses revealed a clear relationship between growth form and photoprotective capacity, and identified fast regulatory physiological photoprotective traits (that is, non-photochemical quenching (NPQ) and the xanthophyll cycle (XC)) as key traits defining the functional light response of these diatoms. EPM-NM and motile EPL showed the highest and lowest NPQ, respectively, with EPM-M showing intermediate values. Like EPL, TYCHO had low NPQ, irrespective of whether they were grown in benthic or planktonic conditions, reflecting an adaptation to a low light environment. Our results thus provide the first experimental evidence for the existence of a trade-off between behavioural (motility) and physiological photoprotective mechanisms (NPQ and the XC) in the four major intertidal benthic diatoms growth forms using unialgal cultures. Remarkably, although motility is restricted to the raphid pennate diatom clade, raphid pennate species, which have adopted a non-motile epipsammic or a tychoplanktonic life style, display the physiological photoprotective response typical of these growth forms. This observation underscores the importance of growth form and not phylogenetic relatedness as the prime determinant shaping the physiological photoprotective capacity of benthic diatoms.

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Section: Photoprotection In Intertidal Benthic Diatomsmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Growth form defines physiological photoprotective capacity in intertidal benthic diatoms

et al. 2014

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“…The larger pool size recorded in the Tagus than in the Eden estuary also corresponds with the higher average irradiance conditions in the Tagus (Table 1). Seasonal photoacclimation patterns of microphytobenthos have been reported previously (Blanchard & Cariou-Le Gall, 1994;Barranguet et al, 1998;Kromkamp et al, 1998), but the underlying physiological mechanisms have not been well described so far.…”

Section: Discussionmentioning

confidence: 99%

“…Accurate pigment data on microphytobenthic photoacclimation are still scarce and photosynthetic responses have been studied mainly by variable fluorescence analyses (e.g. Kromkamp et al, 1998;Perkins et al, 2001). Serodio et al (2005) were the first to measure the xanthophyll pigments and to link the presence of diatoxanthin to non-photochemical quenching signals.…”

Section: Introductionmentioning

confidence: 99%

Photoacclimation in microphytobenthos and the role of xanthophyll pigments

Leeuwe¹,

Brotas

Consalvey

et al. 2008

European Journal of Phycology

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Estuarine microphytobenthos are frequently exposed to excessively high irradiances. Photoinhibition in microalgae is prevented by various photophysiological responses. We describe here the role of the xanthophyll pigments in photoacclimation. The pigment composition of the microphytobenthos was studied in three European estuaries (Barrow, Ireland; Eden, UK; Tagus, Portugal). Using HPLC-analyses, microscale changes in biomass and pigment composition were monitored over short (hourly) and long (seasonal) time scales. In the Barrow estuary, the biomass of microphytobenthos (measured as chlorophyll a) increased significantly in the top 400-500 mm of the sediment surface within 1 h of emersion; simultaneously, the xanthophyll pool size (diadinoxanthin plus diatoxanthin, dd þ dt) almost doubled. A more gradual conversion of dd into dt was observed, with the dt:dd ratio increasing from <0.1 at the start of emersion to >0.3 after 3 h emersion. Similar trends in the dt:dd ratio were observed in the surface sediments of the Eden and the Tagus estuaries. Higher ratios were recorded in the Tagus estuary, which may be explained by higher incident irradiance. In addition, seasonal studies carried out in the Eden and Tagus estuaries showed that the xanthophyll pool size increased by 10% in the summer months. The pool size was highest in the Tagus estuary. Concurrently, high values for the de-epoxidation state were recorded, with values for dt/(dt þ dd) > 0.35 recorded in the summer. At the Eden, the ratio never exceeded 0.3. The de-epoxidation state was higher in winter than in summer, which was ascribed to the low winter temperatures. During a vertical migration study, a negative relationship between chlorophyll a and the de-epoxidation state was observed. It is suggested that this relationship originates from 'micro-migration' within the biofilm. Migration within the euphotic zone may provide an alternative means for cells to escape photodamage. In this paper, we propose that both xanthophyll cycling and 'micro'-migration play an important role in photoacclimation and it appears that these processes operate in parallel to regulate the photosynthetic response.

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“…Such a coefficient was estimated from the highest irradiance (690 µmol m -2 s -1 ) applied when obtaining the light response curves. The calculations followed Schreiber et al (1994) and Kromkamp et al (1998), and the terminology for chl fluorescence described by van Kooten and Snel (1990) was adopted. Curves of PI were generated on the basis of rETR and the respective parameters were calculated by the equation of Platt et al (1980): αETR, P max (rETR max ), I k , I c and photoinhibition parameter (βETR).…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic characteristics of a tropical population of Nitella cernua (Characeae, Chlorophyta)

Vieira

Necchi

2006

Braz. J. Plant Physiol.

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Photosynthetic characteristics (assessed by chlorophyll fluorescence and O 2 evolution) were analysed monthly during one year in a tropical population of Nitella cernua from southeastern Brazil (20 o 50 32" S, 49 o 26 15" W). Parameters derived from photosynthesis-irradiance (PI) curves by fluorescence (high values of the photosynthetic parameter, I k , and lack or low values of photoinhibition, β) suggested adaptation to high irradiance, whereas those by O 2 evolution showed a different pattern (low values of I k and compensation irradiance, I c , high values of photosynthetic efficiency, α, and photoinhibition). Parameters from PI curves by O 2 evolution suggested light acclimation: I k and maximum photosynthetic rate, P max (as rETR, relative electron transfer rate) increased, whereas α became significantly lower under higher irradiance (winter). This pattern is an adjustment of both number and size of photosynthetic units. Photosynthetic performance assessed by fluorescence revealed two seasonal periods: higher values of P max (rETR), I k and nonphotochemical quenching from October to March (rainy season), and lower values from April to October (dry season). Temperature responses were observed only in summer, but temperature optima were different between methods: peaks of net photosynthesis occurred at 20 o C, whereas rETR increased towards higher temperatures (up to 30 o C). Dark respiration increased with higher temperatures. Current velocity had a stimulatory effect on photosynthetic rates, as suggested by positive correlations with P max (rETR) and α. pH experiments revealed highest net photosynthetic rates under pH 4.0, suggesting higher affinity for CO 2 than HCO 3 -. This broad range of responses of photosynthetic characteristics of this N. cernua population to irradiance, temperature, and pH/inorganic carbon reflects a wide tolerance to variations in these environmental variables, which probably contribute to the wide distribution of this species. Key words: charophytes, irradiance, Nitella, pH, photosynthesis, seasonal dynamics, temperature Características fotossintéticas de uma população tropical de Nitella cernua (Characeae, Chlorophyta): Foram analisadas mensalmente, durante um ano, as características fotossintéticas (por meio de fluorescência da clorofila e evolução de O 2 ) de uma população tropical de Nitella cernua do sudeste do Brasil (20 o 50 32" S, 49 o 26 15" W). Parâmetros derivados das curvas fotossíntese-irradiância (FI) por fluorescência (alto valor do parâmetro de saturação de luz, I k , e ausência ou baixa fotoinibição, β) sugeriram adaptação a alta irradiância, enquanto aqueles por evolução de oxigênio mostraram adaptações à sombra (baixo valores de I k e de irradiância de compensação, I c , altos valores de eficiência fotossintética, α, e β). Parâmetros das curvas FI por oxigênio sugeriram aclimatação à luz: I k e taxa fotossintética máxima, F max (analisada como rETR, taxa relativa de transporte de elétrons) aumentaram e α foi significativamente menor sob irradiância mais alta (i...

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Determination of microphytobenthos PSII quantum efficiency and photosynthetic activity by means of variable chlorophyll fluorescence

Cited by 204 publications

References 29 publications

Growth form defines physiological photoprotective capacity in intertidal benthic diatoms

Growth form defines physiological photoprotective capacity in intertidal benthic diatoms

Photoacclimation in microphytobenthos and the role of xanthophyll pigments

Photosynthetic characteristics of a tropical population of Nitella cernua (Characeae, Chlorophyta)

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