Determination of meningococcal polysaccharides by capillary zone electrophoresis

A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines

“…All of the three lots of the experimental vaccine were found into the accepted range (±30%) of CP content and coefficients of variation (CVs) were <10% compared to the acceptance criterion of <15% CV for immunoassays [24]. A recent paper described the use of HPAEC-PAD for MenX polysaccharide quantitation [12], however, this method require prior conversion to monosaccharides by acid digestion and the use of strong acids, often at elevated temperature, represents a potential safety concern for laboratory staff and has a cost associated with material disposal [14].…”

“…Chemical methods like determination of total phosphorus content [9] and sialic acid content [10] has been used. Alternatively, other methods such as high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) [11,12] and capillary zone electrophoresis (CZE) [13,14] have been reported.…”

Vaccine Analysis: Strategies, Principles, and Control

“…Separation can be performed using various technologies, for example by HPLC, HPAEC-PAD/PED (Lei et al 2000), GC, GC-MS , or CE (Lamb et al 2005 Polysaccharide is hydrolyzed to monosaccharide components and then the fluorescent label is added by reductive amination to the released monosaccharides. The methods depend on specific hydrolysis conditions to preserve specific structural aspects of the component monosaccharides.…”

“…Capillary Zonal Electrophoresis (CZE) has been shown to be a robust technique for the separation and identification of a mixture of group A, C, Y, and W-135 polysaccharides (Lamb et al 2005); additionally quantitation was achieved without the need for colorimetric assays or composition analysis. Capillary Zonal Electrophoresis (CZE) has been shown to be a robust technique for the separation and identification of a mixture of group A, C, Y, and W-135 polysaccharides (Lamb et al 2005); additionally quantitation was achieved without the need for colorimetric assays or composition analysis.…”

“…The ability to characterize molecules at the atomic level has allowed NMR spectroscopy to play a leading role in the structural identification of several new pneumococcal serotypes discovered within serogroups, which were considered to be well characterized by serological methods (Calix et al , 2012. Capillary zone electrophoresis (CZE) has been shown to be a robust technique for the separation and identification of a mixture of group A, C, Y, and W polysaccharides (Lamb et al 2005); additionally quantification could be achieved without the need for colorimetric assays or composition analysis (Sect. 8.3.1).…”