Determination of LC50 value of Nicotiana tabacum L. extract against Gryllus bimaculatus imago and Galleria mellonella larvae

Andjani, Hannah Natasha; Sentosa, Yogi; Yati, Kori; Jufri, Mahdi; Fauzantoro, Ahmad; Gozan, Misri

doi:10.1063/1.5139361

Cited by 8 publications

(6 citation statements)

References 16 publications

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“…Pembuatan formulasi nano larvasida tembakau dilakukan berdasarkan metode yang dikembangkan dengan modifikasi, [8][9]17,18 pada penelitian ini pembuatan nanoinsektisida perak menggunakan jenis pelarut akuades. Ekstrak tembakau 320 mg ditambah pelarut sebanyak 80 ml dicampur dengan larutan AgNO3 600 ml air dan diaduk dengan stirrer kecepatan 800 rpm selama tiga puluh menit sampai homogen.…”

Section: Pembuatan Formulasi Nanoinsektisidaunclassified

“…setelah itu disentrifugasi 300 rpm selama tiga puluh menit, serta dihomogenkan dengan menggunakan sonikator selama sepuluh menit. [8][9]17,18 Kemudian dilakukan karakterisasi nanopartikel perak dilakukan dengan analisis Scanning Electron Michroscope (SEM) untuk mengetahui struktur kristal dan morfologi serta ukuran butir dihitung dengan menggunakan Particle Size Analyzer (PSA). 18 Uji Aktivitas terhadap Nyamuk Ae.…”

Section: Pembuatan Formulasi Nanoinsektisidaunclassified

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Uji Efikasi Nanoinsektisida Komposisi Perak Tembakau (Nicotiana tabacum) terhadap Aedes aegypti

Handayani

Prastowo

Boesri

et al. 2020

mpk

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Abstract Vector control that used insecticides need to be substituted, because it has a negative impact for the environment and have been resistance for some areas, so it was necessary to find alternative insecticides. One of the natural insecticides was tobacco (Nicotiana tabacum). The chemical content of tobacco leaves included alkaloids, saponins, and flavonoids. Nicotine was an alkaloid group compound in tobacco, thatwas a nerve poison that reacts quickly and can act as a contact poison in insects, to add the effectiveness it’s necessary change to nano particle with silver. Besides, this test used two solvents with different contains of mineral to compare the effectiveness. This study aimed to test effication of nanoinsecticide from formulation tobacco (Nicotiana tabacum) and silver particle for vector control of larvae Ae.aegypti. It was held at the Center for Research and Development of Disease Vector and Reservoir (B2P2VRP) with an experimental method. The results of the study showed 1,153 ppm LC50, 1,719 ppm LC90 and 1,925 ppm LC90 on solvent distilled water. LC50 of 1,641 ppm, LC90 of 10,741 ppm and LC90 of 18,295 ppm in solvent aquademineralization. Measurements of tobacco nanoinsecticides are known to be 89,2 – 112,0 run in aquadest and 89,2 -112,0 μm in aquademineralization solvents 79,0 – 143,7μm. Abstrak Pengendalian vektor menggunakan insektisida kimiawi perlu disubstisusi karena berdampak buruk pada lingkungan dan menyebabkan resistensi di beberapa daerah, sehingga perlu untuk mendapatkan insektisida alternatif yang ramah lingkungan. Salah satu tanaman insektisida alam, adalah tembakau (Nicotiana tabacum). Kandungan kimia tembakau meliputi alkaloid, saponin, dan flavanoid. Nikotin termasuk senyawa alkaloid dalam tembakau merupakan racun syaraf dengan reaksi cepat serta dapat berfungsi sebagai racun kontak serangga. Namun, untuk menambah daya bunuhnya sebagai larvasida maka ukuran partikel alkaloid perlu dipecah contohnya dengan penambahan perak. Pemilihan perak sebagai pembentuk molekul nano, sedangkan pelarut yang digunakan yaitu akuades dan akuademineralisasi. Kedua pelarut yang digunakan merupakan pelarut standar yang mempunyai daya kelarutan tinggi dengan perbedaan kandungan mineral. Penelitian ini bertujuan untuk uji efikasi nanoinsektisida tembakau (Nicotiana tabacum) yang diformulasikan dengan perak sebagai sarana pengendalian Aedes aegypti stadium pradewasa. Penelitian dilaksanakan di Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) dengan metode eksperimental murni. Hasil penelitian didapatkan LC50 1,153 ppm, LC90 1,719 ppm pada pelarut akuademineraliasi dan LC90 1,925 ppm pada pelarut akuades. LC50 1,641 ppm, LC90 10,741 ppm dan LC90 18,295 ppm pada pelarut akuademineralisasi. Pengukuran partikel nanoinsektisida daun tembakau diketahui berukuran 89,2 - 112,0 nm pada pelarut akuades dan 89,2 -112,0 nm pada pelarut akuademineralisasi 79,0 - 143,7nm.

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Section: Pembuatan Formulasi Nanoinsektisidaunclassified

Uji Efikasi Nanoinsektisida Komposisi Perak Tembakau (Nicotiana tabacum) terhadap Aedes aegypti

Handayani

Prastowo

Boesri

et al. 2020

mpk

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“…e use of plant-based pesticides provides a double advantage, i.e., producing safe products to humans as well as no pollution to the environment [6]. Previous studies on Nicotiana tabacum extracts produced by using solvent extraction as well as pyrolysis methods have shown a significant effect of those extracts on various fungus and insects [7][8][9][10][11][12]. e extract reduces the protein content of robusta skin, resulting in reduced attack by insects [11,12].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Biopesticide and Fusarium oxysporum f.sp. vanillae on the Nutrient Content of Binucleate Rhizoctonia-Induced Vanilla Plant

Haryuni

Harahap

Supartini

et al. 2020

International Journal of Agronomy

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Binucleate Rhizoctonia (BNR) fungi are essential for the germination of vanilla seeds. Chemical control of the soil-borne pathogen might adversely affect BNR. The purpose of this study is to determine the effect of Nicotiana tabacum extract biopesticides and Fusarium oxysporum f.sp. vanillae (Fusarium) on vanilla plant nutrient content induced by BNR. Materials and Methods. The research design was completely randomized design with two factors and three replications. The first factor was biopesticide (dosage of 0, 10, 20, and 30 ml/seedling), and the second factor was the application of Fusarium. Results. The increase in the nitrogen, phosphorus, and potassium content of vanilla was affected by biopesticides and Fusarium inoculation. Fusarium inoculation has no significant effect on nitrogen and phosphorus levels but significantly affects potassium levels. The biopesticide dosage is significant for nitrogen, phosphorus, and potassium. The interaction of biopesticides with Fusarium inoculation did not significantly affect the parameters of nitrogen and phosphorus content, but significantly affected potassium content. Conclusion. The application of biopesticides and Fusarium inoculation after induction of BNR can increase nitrogen, phosphorus, and potassium content of vanilla plants.

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“…e cabbage looper attack was only 9.32% that occurred on the field, while the attack to the control was 34.23% [30]. Other previous studies also reported that Nicotiana tabacum L. leaf extract obtained from Ethanolic Heat Reflux Extraction (EHRE) had shown insecticidal activity against some agricultural pests such as Gryllus bimaculatus (cricket), Galleria mellonella (greater wax moth) larvae, Tenebrio molitor (mealworm beetle) larvae, and Zophobas morio (darkling beetle) larvae at LC 50 values of 38.5 mg/ml, 36.6 mg/ml, 21.1 mg/ml, and 71.1 mg/ml, respectively [31,32]. e top fraction bio-oil of N. tabacum was also reported as a biorepellent to protect human skin from mosquito bites.…”

Section: Introductionmentioning

confidence: 99%

Field Efficacy of Nicotiana tabacum L. var Virginia Extract against Coffee Borer Beetle (Hypothenemus hampei) Attacking Coffee Berries in Plantation Area

Harahap

Fauzantoro²,

Haryuni

et al. 2020

International Journal of Agronomy

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The extract of Nicotiana tabacum L. var Virginia origin of Ponorogo (EHRE-Nt) was applied under field conditions against the coffee borer beetle Hypothenemus hampei (CBB). Three extract formulations containing 150, 300, and 450 ml of EHRE-Nt in 100 liters of water were prepared and sprayed on 0.5 hectares each. A nontreatment was applied as a control on 0.5 hectares of plantation. The intensity of the CBB attacks was observed for six weeks on a Robusta coffee plantation in the Kalibening area, South Semarang, Central Java, Indonesia. An extract formulation containing 450 ml of EHRE-Nt resulted in the lowest intensity of CBB attacks from weeks one to six (1.6% to 2.2%). The attacks of the control field were 11.3% to 13.5%. The highest efficacy level of 85.4% was obtained with the 450 ml EHRE-Nt formulation. These results demonstrate that EHRE-Nt has potential as a bioinsecticide for coffee berries from CBB attacks.

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Determination of LC50 value of Nicotiana tabacum L. extract against Gryllus bimaculatus imago and Galleria mellonella larvae

Cited by 8 publications

References 16 publications

Uji Efikasi Nanoinsektisida Komposisi Perak Tembakau (Nicotiana tabacum) terhadap Aedes aegypti

Uji Efikasi Nanoinsektisida Komposisi Perak Tembakau (Nicotiana tabacum) terhadap Aedes aegypti

The Effects of Biopesticide and Fusarium oxysporum f.sp. vanillae on the Nutrient Content of Binucleate Rhizoctonia-Induced Vanilla Plant

Field Efficacy of Nicotiana tabacum L. var Virginia Extract against Coffee Borer Beetle (Hypothenemus hampei) Attacking Coffee Berries in Plantation Area

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