Determination of In-vitro Optimum Conditions of Xylanase Enzyme Activity Produced by Orpinomyces sp. and Neocallimastix sp.

Abstract

This study was aimed to determine the in-vitro optimum conditions of xylanase enzyme activity produced by Orpinomyces sp. and Neocallimastix sp. In the study, xylanase enzyme activity was investigated in terms of extracellular and intracellular total activity (TA) and specific activity (SA) levels at different time (day), pH and temperature levels. Orpinomyces sp. and Neocallimastix sp. when different days (time) were considered in terms of xylanase enzyme activity of fungi species, it was determined that there was a statistically significant positive correlation between TA=0.732 and SA=0.546 (p<0.01). It was determined that there was a statistically positive and significant relationship between TA=0.622 and SA= 0.520 at different pH levels. This situation differs in terms of temperature levels. It was determined that there was a statistically negative significant relationship between genders and SA=-0.354 (p<0.05). In this study, it is thought to contribute by determining the optimum conditions in in-vitro and industrial uses.