Determination of glyphosate in soil/sludge by high performance liquid chromatography

Lin, Sun; Kong, Deyang; Gu, Wanyi; Guo, Xinyan; Tao, Wenqi; Shan, Zhengjun; Wang, Ying; Wang, Na

doi:10.1016/j.chroma.2017.04.018

Cited by 75 publications

(38 citation statements)

References 15 publications

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“…Soil samples for each treatment were dried in an oven at 80 • C for 5 h because of differences in humidity; 10.0 g of soil was accurately weighted for each treatment and extracted with 25 mL of a solution containing sodium phosphate (0.4 M) and trisodium citrate (0.4 M) [33]. To perform the standard method, each sample was subjected to spiking with a glyphosate standard at the following concentrations: 0 mg/mL, 5.56 × 10 4 mg/mL, 8.33 × 10 4 mg/mL, and 1.11 × 10 5 mg/mL.…”

Section: Extraction Of Glyphosate From the Soil And Standard Additionmentioning

confidence: 99%

Glyphosate Bioremediation through the Sarcosine Oxidase Pathway Mediated by Lysinibacillus sphaericus in Soils Cultivated with Potatoes

et al. 2019

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Glyphosate-based herbicides (GBH) use has increased drastically over the last decade. This is true especially for potato crops due to their fast harvest cycle and high market demand. In 2015, the World Health Organization (WHO) classified glyphosate and its breakdown product amidomethylphosphonic acid (AMPA) as probably carcinogenic to humans, and it has been reported that these compounds disrupt the ecological and nutritional equilibrium of soils. However, microorganisms with the sarcosine oxidase gene, such as Lysinibacillus sphaericus, can degrade glyphosate through the Carbon-Phosphorus (C-P) pathway without leading to AMPA production. The aim of this study was to evaluate the addition of the plant growth-promoting bacteria (PGPB) L. sphaericus as a bioremediation agent in a potato crop sprayed with a GBH, in conjunction with the nitrogen fixation activity mediated by the bacteria. To that end, a GBH solution was used to treat a potato field, and different treatments (glyphosate (G), bacteria (B), bacteria+glyphosate (BG), and negative control (C)) were evaluated by measuring the glyphosate, AMPA, nitrates, and ammonium concentrations. BG treatment showed a 79% reduction of glyphosate concentration in soil, leading to minimal AMPA production, compared to the 23% reduction observed after G treatment. Furthermore, the ammonium concentrations were significantly higher in samples treated with BG and in C samples (p < 0.005). Therefore, we propose the addition of L. sphaericus as a good bioremediation strategy for soils sprayed with GBH.

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Section: Extraction Of Glyphosate From the Soil And Standard Additionmentioning

confidence: 99%

Glyphosate Bioremediation through the Sarcosine Oxidase Pathway Mediated by Lysinibacillus sphaericus in Soils Cultivated with Potatoes

et al. 2019

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“…Several studies concerning the determination of these herbicides and corresponding metabolites with liquid chromatography-tandem mass spectrometry (LC-MS/ MS) were proposed [19][20][21]. In general, due to their zwitterionic characteristics [22], derivatization of these compounds are typically required for the analysis, while the derivatization process is time-consuming and complicated [19,23,24].…”

Section: Introductionmentioning

confidence: 99%

Sensitive, rapid and non-derivatized determination of glyphosate, glufosinate, bialaphos and metabolites in surface water by LC–MS/MS

Guo

Gao²,

Guo

et al. 2019

SN Appl. Sci.

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Glyphosate, glufosinate and bialaphos are widely used worldwide as herbicides. It has been reported that they can transfer into environmental water bodies, posing health threat to human. Thus, a sensitive and rapid analysis methodology for these compounds and corresponding metabolites is highly necessary. Due to their zwitterionic nature, previously reported methods for determination of these compounds generally require derivatization. Also, preconcentration step is typically needed in order to obtain high sensitivity. Herein, we report a very convenient and sensitive assay for these herbicides as well as corresponding metabolites in surface water. With judicious tuning of chromatographic conditions, very low LODs were achieved without derivatization or preconcentration step. The LODs were 0.15 ng mL −1 for glyphosate, glufosinate, MPPA and bialaphos, 0.1 ng mL −1 for AMPA. The LOQs were 0.5 ng mL −1 for glyphosate, glufosinate, MPPA and bialaphos, 0.3 ng mL −1 for AMPA. Recoveries ranging in 90.3-102.8% were obtained. The intra-day relative standard deviations (RSDs) ranged in 4.0-5.6%, while the inter-day RSDs ranged in 4.7-6.7%. The ME (n = 6) ranged from 92.6 to 97.2%. This assay was applied to real samples of surface water. This method is very promising for application in determination and routine monitoring of these compounds in environmental water bodies.

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“…The development of analytical methods suitable to fulfill the requirement of determining imazethapyr in soil has attracted widespread interest. The methods for determination of herbicide residues in soil include solid-phase extraction [ 7 ], dispersive solid-phase extraction [ 8 , 9 ], magnetic solid-phase extraction (MSPE) [ 10 ], multiple monolithic fiber solid-phase microextraction (MMF-SPME) [ 11 ], high-performance liquid chromatography (HPLC) [ 12 ], high-performance liquid chromatography with mass spectroscopy (HPLC-MS/MS) [ 13 ], gas chromatography coupled with an electron capture detector (GC-ECD) [ 14 ], and molecular imprinting [ 15 , 16 ]. Compared with other methods, molecular imprinting has emerged as a powerful technique [ 17 ] for the preparation of tailor-made recognition material owing to its high affinity and specificity [ 18 – 21 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Imazethapyr Surface Molecularly Imprinted Polymers for Its Selective Recognition of Imazethapyr in Soil Samples

Zhou

Yang

et al. 2018

Journal of Analytical Methods in Chemistry

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A novel strategy based on imazethapyr (IM) molecular-imprinting polymers (MIPs) grafted onto the surface of chloromethylation polystyrene resin via surface-initiated atom transfer radical polymerization (SI-ATRP) for specific recognition and sensitive determination of trace imazethapyr in soil samples was developed. The SI-ATRP was performed by using methanol-water (4 : 1, v/v) as the solvent, acrylamide as the functional monomer, trimethylolpropane trimethacrylate (TRIM) as the cross-linker, imazethapyr as the template, and CuBr/2,2′-bipyridine as the catalyst. The resulting MIPs were characterized by elemental analysis, Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Then, the binding selectivity, adsorption capacity, and reusability of the MIPs were evaluated. The results indicated that the prepared MIPs exhibited specific recognition and high selectivity for imazethapyr. The MIPs were further used as solid-phase extraction (SPE) materials coupled with high-performance liquid chromatography (HPLC) for selective extraction and detection of trace imazethapyr from soil samples. The results showed that good linearity was observed in the range of 0.10–5.00 μg/mL, with a correlation coefficient of 0.9995. The limit of detection (LOD) of this method was 15 ng/g, and the extraction recoveries of imazethapyr from real samples were in the range of 91.1–97.5%, which proved applicable for analysis of trace imazethapyr in soils. This work proposed a sensitive, rapid, and convenient approach for determination of trace imazethapyr in soil samples.

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Determination of glyphosate in soil/sludge by high performance liquid chromatography

Cited by 75 publications

References 15 publications

Glyphosate Bioremediation through the Sarcosine Oxidase Pathway Mediated by Lysinibacillus sphaericus in Soils Cultivated with Potatoes

Glyphosate Bioremediation through the Sarcosine Oxidase Pathway Mediated by Lysinibacillus sphaericus in Soils Cultivated with Potatoes

Sensitive, rapid and non-derivatized determination of glyphosate, glufosinate, bialaphos and metabolites in surface water by LC–MS/MS

Preparation of Imazethapyr Surface Molecularly Imprinted Polymers for Its Selective Recognition of Imazethapyr in Soil Samples

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