Determination of fatty acid profile in ram spermatozoa and seminal plasma

Díaz, Rommy; Torres, Mariana Andrade; Bravo, S.; Sánchez, Raúl; Sepúlveda, Néstor

doi:10.1111/and.12506

Cited by 14 publications

(15 citation statements)

References 15 publications

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“…The testicular fatty acid profile varies between species and the predominant PUFA may be DHA (e.g., humans, monkeys and ruminants) (Díaz, Torres, Bravo, Sanchez, & Sepúlveda, ; Esmaeili et al., ), 22:5n6 (e.g., rodents) (Mašek & Starčević, ) or 22:4n6 (e.g., domestic poultry) (Kelso, Cerolini, Speake, Cavalchini, & Noble, ). Therefore, species differences in the fatty acid profile of testicular tissue must be considered to correctly interpret the testicular fatty acid profile and its possible influence on testicular structure.…”

Section: Discussionmentioning

confidence: 99%

Effects of different n6/n3 ratios and supplementation with DHA and EPA on the testicular histology and lipogenesis in streptozotocin-treated rats

et al. 2018

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The aim of this study was to investigate the possible influence of different dietary n6/n3 ratios and DHA/EPA addition on the testis histology, antioxidative status and lipogenesis of streptozotocin-treated rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (55 mg/kg). The rats were divided into five groups: CON (n6/n3 ratio, 7), CON-DM1 (STZ; n6/n3 ratio, 7), N3-DM1 (STZ; n6/n3 ratio, 1), N6-DM1 (STZ; n6/n3 ratio, 60) and DHA-DM1 (STZ; n6/n3 ratio, 1; added DHA/EPA). Antioxidative status was improved in the DHA-DM1 group. Seminiferous tubule diameter, testicular pathohistological scoring and total lipid content were improved in the N6-DM1 group compared to the other streptozotocin-treated groups. Streptozotocin treatment induced changes in testis fatty acid profile depending on n6/n3 ratio. The fatty acid profile of N6-DM1 group was characterised by similar or increased values for n6 fatty acids compared to the CON group, while the DHA-DM1 group had the lowest content of n6 fatty acids. The content of n3 fatty acids was increased in the N3-DM1 and DHA-DM1 groups. These results suggest that a n6/n3 ratio could significantly influence testicular antioxidative status, histology and lipogenesis and that these effects vary depending on the supplemented fatty acid.

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Section: Discussionmentioning

confidence: 99%

Effects of different n6/n3 ratios and supplementation with DHA and EPA on the testicular histology and lipogenesis in streptozotocin-treated rats

et al. 2018

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“…Ram sperm membrane has a low cholesterol/phospholipid ratio, low cardiolipin, phosphatidylserine, ethanolamine plasmalogen, and phosphatidylethanolamine concentrations, and a high content of phospholipids with PUFA, making them highly susceptible to cryodamage ( 49 – 53 ). Ram sperm and seminal plasma have 1.8 and 1.8% total lipids, respectively ( 54 ). SFA was 66.6 and 49.9% in seminal plasma and ram sperm, respectively ( 54 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of adding mixed chicken and quail egg yolks to the cryodiluent on the quality of ram semen before and after cryopreservation

et al. 2022

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The effects of adding mixed chicken and Japanese quail egg yolks (EYs) to the cryodiluent on the quality of ram semen before freezing and post-thawing were evaluated. Additionally, the composition of chicken and quail egg EYs and their mixture were analyzed for results explanation. The semen was collected from rams (n = 5) and extended with cryodiluent containing the EY of chicken, quail or their mixture (1:1). The extended semen was chilled slowly to 5 °C within 2 h and equilibrated for 2 h, before frozen on the liquid nitrogen vapor and cryopreserved at −196 °C. The straws were evaluated before freezing and post-thawing for sperm motility, vitality and abnormality besides plasma-membrane and DNA integrities. The moisture, ash, protein, and fatty acid (FA) contents of chicken EY, quail EY and their mixture were analyzed. Sperm vitality, plasma membrane integrity and DNA integrity before freezing were significantly (P < 0.05) higher in quail EY than chicken EY and mixed EYs cryodiluent. The chicken EY extender significantly improved the vitality, plasma membrane and DNA integrities of post-thawed ram semen in comparison with quail EY or mixed EYs extenders. While, the post-thawing sperm abnormalities was lower (P ≤ 0.05) in quail EY than chicken EY and mixed EYs cryodiluent. The post-thawing sperm motion kinetics parameters were higher in quail EY than chicken EY and mixed EYs cryodiluent. The highest percentages of moisture, ash, saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) were detected in quail EY had. While, the highest percentages of fat, protein and polyunsaturated fatty acids (PUFAs) were detected in chicken EY. In conclusion, using of chicken EY can improve total motility, vitality, plasma membrane integrity and DNA integrity of cryopreserved ram semen. While, using of quail EY can improve sperm abnormalities and kinetic motion parameters of cryopreserved ram semen. Mixing chicken and quail EYs added no value for post-thawing ram semen parameters.

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“…PUFAs are essential sperm cell membrane constituents and can influence their fluidity and integrity [ 14 , 87 ]. The increase in the ω-3 in the sperm plasma membrane phospholipids promotes adequate antioxidant properties, which reduce the risk of damage to sperm cells [ 88 , 89 ]. Lower concentrations of omega-3 have been found in the sperm cells of infertile men [ 55 ].…”

Section: Diet and Male Reproductive Healthmentioning

confidence: 99%

Effect of Paternal Diet on Spermatogenesis and Offspring Health: Focus on Epigenetics and Interventions with Food Bioactive Compounds

et al. 2022

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Infertility is a growing public health problem. Consumption of antioxidant bioactive food compounds (BFCs) that include micronutrients and non-nutrients has been highlighted as a potential strategy to protect against oxidative and inflammatory damage in the male reproductive system induced by obesity, alcohol, and toxicants and, thus, improve spermatogenesis and the fertility parameters. Paternal consumption of such dietary compounds could not only benefit the fathers but their offspring as well. Studies in the new field of paternal origins of health and disease show that paternal malnutrition can alter sperm epigenome, and this can alter fetal development and program an increased risk of metabolic diseases and breast cancer in adulthood. BFCs, such as ascorbic acid, α-tocopherol, polyunsaturated fatty acids, trace elements, carnitines, N-acetylcysteine, and coenzyme Q10, have been shown to improve male gametogenesis, modulate epigenetics of germ cells, and the epigenetic signature of the offspring, restoring offspring metabolic health induced by stressors during early life. This indicates that, from a father’s perspective, preconception is a valuable window of opportunity to start potential nutritional interventions with these BFCs to maximize sperm epigenetic integrity and promote adequate fetal growth and development, thus preventing chronic disease in adulthood.

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Determination of fatty acid profile in ram spermatozoa and seminal plasma

Cited by 14 publications

References 15 publications

Effects of different n6/n3 ratios and supplementation with DHA and EPA on the testicular histology and lipogenesis in streptozotocin-treated rats

Effects of different n6/n3 ratios and supplementation with DHA and EPA on the testicular histology and lipogenesis in streptozotocin-treated rats

Effects of adding mixed chicken and quail egg yolks to the cryodiluent on the quality of ram semen before and after cryopreservation

Effect of Paternal Diet on Spermatogenesis and Offspring Health: Focus on Epigenetics and Interventions with Food Bioactive Compounds

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