Determination of ethyl glucuronide in human hair by SPE and LC–MS/MS

Janda, Ines; Weinmann, Wolfgang; Kuehnle, Thorsten; Lahode, Martina; Alt, Andreas

doi:10.1016/s0379-0738(02)00163-9

Cited by 109 publications

(84 citation statements)

References 7 publications

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“…However, SPE which is also used in other published methods [13,[19][20][21][22][23] requires extra time and results in additional costs. To maintain a similar extraction procedure with direct injection of the aqueous hair extract into the LC-MS/MS device, chromatographic separation was optimized.…”

Section: Discussionmentioning

confidence: 99%

Investigations on the influence of different grinding procedures on measured ethyl glucuronide concentrations in hair determined with an optimized and validated LC-MS/MS method

et al. 2012

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Ethyl glucuronide (EtG) analysis in hair is a suitable method for the retrospective determination of previous alcohol consumption. According to the German guidelines, EtG abstinence is improbable at c(EtG) > 7 pg/mg in the proximal 3 cm of scalp hair. The chromatography of the routinely used liquid chromatography-tandem mass spectrometry procedure was optimized by replacing the stationary phase. To simplify sample preparation, two different mills were tested, and an optimized grinding process was developed. The new method was successfully validated according to the guidelines of the German Society of Toxicological and Forensic Chemistry. Despite a simple extraction procedure without any cleaning steps, a very high sensitivity (limit of detection, 1.7 pg/mg; limit of quantitation, 2.3 pg/mg) could be achieved. Competitive analysis showed significantly higher EtG concentrations in pulverized versus cut hair samples. The strong impact of sample preparation on the determined EtG concentrations suggests the introduction of a standardized sample preparation method to produce comparable results.

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Section: Discussionmentioning

confidence: 99%

Investigations on the influence of different grinding procedures on measured ethyl glucuronide concentrations in hair determined with an optimized and validated LC-MS/MS method

et al. 2012

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“…Also, EtG has recently been suggested as a marker of chronic high alcohol intake, measured in hair (64)(65)(66)(67)(68)(69)(70)(71)(72)(73)(74)(75)(76). According to the research so far, the sensitivity and specificity exceeds that of the traditional markers for chronic high alcohol intake, like the liver enzymes aspartate aminotranserase (AST), alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT) as well as carbohydratedeficient transferrin (CDT) (34,35,(77)(78)(79)(80).…”

Section: Non-oxidative Metabolism Of Ethanol; Ethyl Glucuronide and Ementioning

confidence: 99%

A study of ethyl glucuronide in post-mortem blood as a marker of ante-mortem ingestion of alcohol

Høiseth¹,

Karinen²,

Christophersen³

et al. 2007

Forensic Science International

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“…EtG and EtS of a standard sample were thereby detected after about 6.6 and 9.6 min, respectively (Fig. 1 [9,27]. The same is true for the m/z 125 to 97 (HSO 4 -) transition observed in the case of EtS [10,11].…”

mentioning

confidence: 54%

Confirmation analysis of ethyl glucuronide and ethyl sulfate in human serum and urine by CZE‐ESI‐MSⁿ after intake of alcoholic beverages

2011

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CZE coupled to sheath liquid-based electrospray ionization (ESI) and multiple-stage ion trap mass spectrometry (MS(n) ) was used for the confirmation analysis of ethyl glucuronide (EtG) and ethyl sulfate (EtS) in human serum and urine collected after intake of alcoholic beverages. Electrophoretic separations were performed in uncoated fused-silica capillaries using a pH 9.5 ammonium acetate background electrolyte and normal polarity. MS detection of EtG and EtS occurred after negative ionization using a spray liquid containing 0.5% v/v ammonia in isopropanol/water (60:40%, v/v). CZE-MS and CZE-MS² results obtained after injection of solid-phase extracts for EtG and EtS and of diluted urine confirmed the presence of EtG and EtS in samples whose levels were previously determined by CZE with indirect UV detection. Detection limits of each compound were estimated to be around 2.0 (injection of diluted urine) and 0.2 μg/mL (extracts).

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Determination of ethyl glucuronide in human hair by SPE and LC–MS/MS

Cited by 109 publications

References 7 publications

Investigations on the influence of different grinding procedures on measured ethyl glucuronide concentrations in hair determined with an optimized and validated LC-MS/MS method

Investigations on the influence of different grinding procedures on measured ethyl glucuronide concentrations in hair determined with an optimized and validated LC-MS/MS method

A study of ethyl glucuronide in post-mortem blood as a marker of ante-mortem ingestion of alcohol

Confirmation analysis of ethyl glucuronide and ethyl sulfate in human serum and urine by CZE‐ESI‐MSⁿ after intake of alcoholic beverages

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Determination of ethyl glucuronide in human hair by SPE and LC–MS/MS

Cited by 109 publications

References 7 publications

Investigations on the influence of different grinding procedures on measured ethyl glucuronide concentrations in hair determined with an optimized and validated LC-MS/MS method

Investigations on the influence of different grinding procedures on measured ethyl glucuronide concentrations in hair determined with an optimized and validated LC-MS/MS method

A study of ethyl glucuronide in post-mortem blood as a marker of ante-mortem ingestion of alcohol

Confirmation analysis of ethyl glucuronide and ethyl sulfate in human serum and urine by CZE‐ESI‐MSn after intake of alcoholic beverages

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Confirmation analysis of ethyl glucuronide and ethyl sulfate in human serum and urine by CZE‐ESI‐MSⁿ after intake of alcoholic beverages