Determination of diamine oxidase activity by liquid scintillation counting

Okuyama, Tsuneo; Kobayashi, Yutaka

doi:10.1016/0003-9861(61)90141-2

Cited by 285 publications

(83 citation statements)

References 12 publications

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“…The reaction was stopped by the addition of 10 p1 of aminoguanidine M). Fifty p1 of saturated bicarbonate solution was then added to each tube in order to improve the toluene extractability (8). The toluene extraction was performed twice by using 500 p1 of the organic solvent and by allowing the water phase to freeze.…”

Section: Discussionmentioning

confidence: 99%

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Serum Diamine Oxidase Activity in Acute Gastroenteritis in Children

et al. 1985

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ABSTRACT. Serum diamine oxidase (DAO) activities were measured in 20 control patients and in 24 patients with gastroenteritis. Results (mean f SE) were as follows: 1) control patients (n = 20), 36.2 2 5.7 pmol h-' ml-I; 2) gastroenteritis (acute phase) (n = l l ) , 31 f 4.9 pmol h-' ml-I; 3) gastroenteritis (healing phase) (n = 21), 18 +. DAO, diamine oxidaseDiamine oxidase is an enzyme showing a high activity in small intestinal mucosal extracts of humans and other mammalian species (6). The enzyme is localized in the cytosol fraction of mature villous enterocytes (2). Mucosal DAO, through its implication in local polyamine and histamine catabolism, is thought to play a role in cell division, as well as a local protective role against histamine (2, 3). We have shown that a positive correlation exists between mucosal DAO and disaccharidase activities in children with a histologically normal duodenal mucosa (5). This supports the hypothesis that mucosal DAO activity reflects mucosal functional integrity. DAO activity is measurable not only in mucosal extracts but also in serum. Further correlation between serum and ileal activities has been shown to exist in the rat (6). The physiological function of circulating DAO is not known.Acute gastroenteritis is accompanied by mucosal destruction and decreased mucosal disaccharidase activities (1). If serum DAO activity accurately reflects small intestinal functional integrity, as stated above, a decrease of serum activities would be

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Section: Discussionmentioning

confidence: 99%

“…The activity of DAO (EC 1.4.3.6) was estimated by a method close to the radiometric technique described by Okuyama and Kobayashi (8). In this method, [3H]putrescine is used as substrate.…”

Section: Methodsmentioning

confidence: 99%

Serum Diamine Oxidase Activity in Acute Gastroenteritis in Children

et al. 1985

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“…The activity of DAO (EC 1.4.3.6) was estimated by a method close to the radiometric technique described by Okuyama and Kobayashi (6). In this method, ['Hlputrescine is used as substrate.…”

Section: Methodsmentioning

confidence: 99%

“…of aminoguanidine M). 50 of a saturated bicarbonate solution was then added to each tube in order to improve the toluene extractability (6). The toluene extraction was performed twice by using 500 p1 of the organic solvent and by allowing the watery phase to freeze.…”

Section: Sucrase Activity U G -L W E T W E I G H T )mentioning

confidence: 99%

Diamine Oxidase and Disaccharidase Activities in Small Intestinal Biopsies of Children

et al. 1984

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SummaryA relationship between disaccharidase and diamine oxidase (DAO) activities was looked for by measuring these enzyme activities in histologically normal small intestinal biopsies of 18 children. The range for disaccharidase activities expressed in U g-' wet weight was 0.1-5.7, 7.1-36.7, and 2.3-8.5 for lactase, maltase, and sucrase, respectively. The range for DAO activities expressed in nmol h-' g-' wet weight was 202-974. Significant correlations were found between disaccharidase and DAO activities (lactase versus DAO: n = 17, r = 0.80, P < 0.001; maltase versus DAO: n = 18, r = 0.70, P < 0.001; sucrase versus DAO: n = 18, r = 0.55, P < 0.05). Our results further support the hypothesis that DAO is a marker of small intestinal functional integrity in children.

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“…Brie¯y, the assay mixture contained: 1.5 ml sodium phosphate buffer, 0.1 M, pH 7.2; 0.3 ml sample; and 0.1 ml substrate, a mixture of putrescine dihydrochloride and 1-4 14 C-putrescine dihydrochloride up to a ®nal concentration of 5 nmol per tube with an activity of 0.1 mCi. The samples were incubated for 60 min at 37 C. The labeled reaction product, 14 C-1-pyridine, was directly extracted in a toluene-based scintillation mixture and assayed for 14 C by a Packard Tricarb 4530 scintillation counter (Okuyama and Kobayashi, 1961). DAO activity was expressed as Uaml (1 U 1 nmol of putrescine dihydrochloride oxidised in 1 h at 37 C pH 7.2).…”

Section: Phd Test and Dao Assaymentioning

confidence: 99%

Histamine plasma levels and elimination diet in chronic idiopathic urticaria

Guida

Martino

et al. 2000

Eur J Clin Nutr

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Objective: The aim of this study was to evaluate the effects of an oligoantigenic and histamine-free diet on patients affected with chronic idiopathic urticaria (CIU). Design: Ten patients with chronic idiopathic urticaria were prescribed an oligoantigenic and histamine-free diet for 21 days, followed by serial and controlled reintroduction of foods during a further 70 days. Modi®cation in clinical illness as well as histamine plasma levels, post-heparin plasma diamine oxidase (DAO) and intestinal permeability were evaluated. Results: The oligoantigenic and histamine-free diet induced a signi®cant improvement of symptoms (P`0.05). Moreover, CIU patients on free diet showed higher histamine plasma levels (P`0.05 vs post-diet and vs controls) that fell to control levels during the oligoantigenic and histamine-free diet. Post-heparin plasma diamine oxidase values were slightly reduced and were unchanged during the diet as well as intestinal permeability, which was always normal in all patients. Conclusions: These data suggest that histamine plays a major role in chronic idiopathic urticaria. The ®nding of normal intestinal permeability suggests that a morphological damage of intestinal mucosa should be excluded in these patients. However, the presence of low levels of post-heparin plasma diamine oxidase may indicate a subclinical impairment of small bowel enterocyte function that could induce a higher sensitivity to histaminerich or histamine-producing food. Descriptors: chronic idiopathic urticaria; diet; histamine; diamine oxidase; intestinal permeability

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Determination of diamine oxidase activity by liquid scintillation counting

Cited by 285 publications

References 12 publications

Serum Diamine Oxidase Activity in Acute Gastroenteritis in Children

Serum Diamine Oxidase Activity in Acute Gastroenteritis in Children

Diamine Oxidase and Disaccharidase Activities in Small Intestinal Biopsies of Children

Histamine plasma levels and elimination diet in chronic idiopathic urticaria

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