Determination of Di(2-ethylhexyl phthalate) released in the blood from PVC line during the hemodialysis procedure

Abstract

The objective of the work is to demonstrate the applicability of the High-Performance Liquid Chromatography (HPLC) on the quantification of the Di(2-ethylhexyl phthalate) (DEHP) released from the Polyvinyl Chloride (PVC) line into the circulating blood during the hemodialysis procedure. As the proposal is not to validate the method, blood samples from five patients and three healthy volunteers were collected, and the plasmas were isolated, prepared and analyzed. The average concentration of DEHP in the patient's plasma was 0.19±0.12 mg/kg of body mass, while in the plasma of the healthy volunteers was 0.003±0.002 µg/kg of body mass. The results showed that the method presented is an alternative to quantify the DEHP in the blood and could assist in the search for alternative materials to apply to extracorporeal circulation equipment. The study was performed according to the national and international standards of ethics in research involving human beings (CEP PUCPR Prot. n.5802).