Determination of Crude Fiber

Neubert, A. M.; Vanamburgh, Fred; John, Jacob

doi:10.1021/ac50148a004

Cited by 7 publications

(2 citation statements)

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“…The method of Neubert, Vanamburgh, and St. John (1940) was used to determine fiber. Briefly, 2.50 g of defatted plant material was added in a flask containing 200 ml of 1.25%, v/v sulfuric acid.…”

Section: Methodsmentioning

confidence: 99%

Morphological, microscopic, and physicochemical studies of Diospyros montana

Khan

Azhar

Shehzadi

et al. 2020

Microscopy Res & Technique

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Adulteration is the root cause of producing not only a chemically and pharmacologically inferior but also in some instances hazardous or poisonous drug. Despite availability of several techniques, microscopy and physicochemical analyses are the most practical approaches for crude drug authentication. Hence, the present study aimed to evaluate morphological, microscopic, and physicochemical properties of root, bark, leaf, and fruit of Diospyros montana Morphological properties were determined by sensory organs, whereas microscopic features of cross‐sections and powders were determined by light and scanning electron microscopy. The proximate and fluorescence analyses were performed using the standard guidelines. The physical examination of fresh, shade‐dried, and powdered material showed no significant change in color. The identifying cellular structures included cuboidal cork, pitted tracheids, scalariform, reticulate and spiral xylary vessels, and rosettes, raphide, and cuboidal calcium oxalate crystals. The stomatal number, stomatal index, vein‐islet and vein‐termination number, and palisade ratio in the leaf were found to be 293.91 ± 32.68 mm−2, 64.18 ± 3.42%, 22.00 ± 3.81 mm−2 and 38.40 ± 5.81 mm−2, and 3.85 ± 0.60, respectively. Total ash, acid insoluble ash, water soluble ash and sulfated ash of leaf (9.00 ± 0.50%, 1.67 ± 0.23%, 2.00 ± 0.22% and 14.50 ± 0.99%, respectively), foaming index of bark and root (111.11 ± 2.11), and swelling index of fruit (19.00 ± 3.45) were higher than the other parts. The powder of different parts showed characteristic colors in the daylight and UV light upon treatment with various regents. The plant was found to be rich in saponins, fibers, and flavonoids. The results of the present study may serve as identifiers of different parts of Diospyros montana.

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Section: Methodsmentioning

confidence: 99%

Morphological, microscopic, and physicochemical studies of Diospyros montana

Khan

Azhar

Shehzadi

et al. 2020

Microscopy Res & Technique

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“…The feed digestibility was calculated on the basis of compositional analysis of d0 and d7 residual feed as per the method of Neubert et al . [14]. Fermented RUSITEC fluid from control and test chambers was collected for TVC estimation until d7.…”

Section: Methodsmentioning

confidence: 99%

Administration of Streptococcus bovis isolated from sheep rumen digesta on rumen function and physiology as evaluated in a rumen simulation technique system

Aphale¹,

Natu

Laldas

et al. 2019

Vet World

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Background and Aim:Little information about the stability and changes of sheep ruminal microbiota due to pathogen intervention in the rumen simulation technique (RUSITEC) is available. This study aimed to investigate the effect of administration of a novel isolated Streptococcus bovis strain on rumen microbiology and physiology. In addition, the isolation of pigment-producing Streptococcus lutetiensis is described.Materials and Methods:Microbial strains were isolated from sheep rumen digesta. An isolated strain of S. bovis was evaluated in the RUSITEC system fed with mixed cattle feed and compared with an in-house developed probiotic formulation (PF), PF 1, containing Bacillus amyloliquifaciens, Bacillus subtilis, and Propionibacterium freudenreichii. The parameters of volatile fatty acid, lactic acid, pH profiling, and the coliform anti-pathogenicity were evaluated to determine the effect of S. bovis on rumen function and physiology.Results:Administration of S. bovis reduced the coliform count by 31.20% from 7.2×1010 colony-forming units (CFU)/mLto 1.7×106 CFU/mL. Agar diffusion assays revealed the extracellular antimicrobial activity of S. bovis against coliforms; Escherichia coli and Salmonella enterica with 12 and 14 mm zones of inhibition, respectively. Simultaneously, an increase of 61.62% in the rumen yeast count was noted. The physiological changes resulted in a 5% reduction in acetic acid concentration from 431 to 405 mg/L.Conclusion:The present research indicates that S. bovis is highly capable of altering rumen physiology and function on colonization and is a key transition microbe to be studied during rumen intervention studies. A decrease in the coliform count could be attributed to extracellular production of a bacteriocin-like substance, as illustrated through agar diffusion assays.

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Über die Rohfaserbestimmung. II

Hirsjärvi

Andersen

1954

Z. Anal. Chem.

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Determination of Crude Fiber

Cited by 7 publications

References 0 publications

Morphological, microscopic, and physicochemical studies of Diospyros montana

Morphological, microscopic, and physicochemical studies of Diospyros montana

Administration of Streptococcus bovis isolated from sheep rumen digesta on rumen function and physiology as evaluated in a rumen simulation technique system

Über die Rohfaserbestimmung. II

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