Determination of collagen-proteoglycan interactions in vitro

Zhu, Wenbo; Iatridis, James C.; Hlibczuk, Veronica; Ratcliffe, Anthony; Mow, Van C.

doi:10.1016/0021-9290(95)00136-0

Cited by 50 publications

References 39 publications

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“…Further, there was an increase in the magnitude of the correlation coefficient when both proteoglycan and collagen were related to the shear modulus, suggesting the formation of a functional cartilage matrix. 44,45 It is noted that while the resulting scaffold mechanical properties in this study were only a fraction of those of the native calcified cartilage interface, 48 the positive correlations between cell-mediated matrix deposition and scaffold mechanical properties demonstrate the potential regeneration of both biomimetic and functional tissue in the hydrogel-ceramic composite scaffold. While the mechanical properties of the alginate + HA scaffold do not yet meet those of calcified cartilage, 9 both scaffold (e.g., HA content) and culturing conditions (e.g., mechanical loading) can be optimized to increase its mechanical properties in future studies.…”

Section: Fig 6 Mineralization Potential and Mineral Distribution (A)mentioning

confidence: 69%

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A Hydrogel-Mineral Composite Scaffold for Osteochondral Interface Tissue Engineering

Khanarian

Jiang²,

Wan³

et al. 2012

Tissue Engineering Part A

Self Cite

102

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Osteoarthritis is the leading cause of physical disability among Americans, and tissue engineered cartilage grafts have emerged as a promising treatment option for this debilitating condition. Currently, the formation of a stable interface between the cartilage graft and subchondral bone remains a significant challenge. This study evaluates the potential of a hybrid scaffold of hydroxyapatite (HA) and alginate hydrogel for the regeneration of the osteochondral interface. Specifically, the effects of HA on the response of chondrocytes were determined, focusing on changes in matrix production and mineralization, as well as scaffold mechanical properties over time. Additionally, the optimal chondrocyte population for interface tissue engineering was evaluated. It was observed that the HA phase of the composite scaffold promoted the formation of a proteoglycan-and type II collagen-rich matrix when seeded with deep zone chondrocytes. More importantly, the elevated biosynthesis translated into significant increases in both compressive and shear moduli relative to the mineral-free control. Presence of HA also promoted chondrocyte hypertrophy and type X collagen deposition. These results demonstrate that the hydrogel-calcium phosphate composite supported the formation of a calcified cartilage-like matrix and is a promising scaffold design for osteochondral interface tissue engineering.

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Section: Fig 6 Mineralization Potential and Mineral Distribution (A)mentioning

confidence: 69%

“…It is well established that the mechanical properties of cartilage are related to collagen and GAG content. 44,45 Therefore, the correlation between mechanical properties (E eq and jG*j) and scaffold collagen and GAG content was determined both individually (GAG or Col) and in combination (GAG + Col), using linear regression models.…”

Section: Discussionmentioning

confidence: 99%

A Hydrogel-Mineral Composite Scaffold for Osteochondral Interface Tissue Engineering

Khanarian

Jiang²,

Wan³

et al. 2012

Tissue Engineering Part A

Self Cite

102

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“…Rheological tests were performed on a RFS-3 rheometer (Rheometric Scientific Inc.) using the parallel-plate configuration. 12 The pilot dynamic shear strain-sweep test at a frequency of 6.28 rad/s indicated 0.1% shear strain was in the linear stressstrain range for the samples with various concentration ratios, and such linearity was confirmed using the dynamic shear strain-sweep test for each test sample before the dynamic shear frequency-sweep test. The dynamic shear frequency-sweep was tested over a range of frequencies from 0.1 to 100 rad/s at a shear amplitude of 0.1%.…”

Section: Rheological Characterizationmentioning

confidence: 81%

Photocrosslinkable polysaccharides based on chondroitin sulfate

Williams

Sun

et al. 2003

J. Biomed. Mater. Res.

180

166

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Abstract:Photopolymerizing hydrogels have demonstrated potential for use as a scaffold in numerous tissueengineering applications. The majority of photopolymerizing hydrogels are made from purely synthetic polymers. The purpose of this study was to synthesize and characterize photopolymerizing hydrogels derived from the biopolymer chondroitin sulfate in order to enhance the bioactivity of the scaffold and potentially improve tissue regeneration. Methacrylate groups were added to chondroitin sulfate, a major component of cartilage, using glycidyl methacrylate. The gels exhibited viscoelastic behavior typical of hydrogels. Cogels based on chondroitin sulfate and poly(ethylene glycol) demonstrated increasing pore size with increasing concentration of chondroitin sulfate as determined by water content, mechanical strength, and morphology using scanning electron microscopy. The chondroitin sulfate hydrogels degraded specifically in the presence of the enzyme chondroitinase. Chondrocytes remained viable after photoencapsulation and incubation in the biogels, suggesting their possible use for cartilage tissue engineering.

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“…The aggregating capacity of isolated proteoglycans in vitro was determined by incubating proteoglycans with 2% hyaluronan. Furthermore, it was stated that combining 2% hyaluronan to aggrecan (w/w) resulted in significant macromolecular formation ( Zhu et al, 1996;O'Halloran and Pandit, 2007). The final concentration of HA present in the hydrogel would be sufficient to allow the formation of aggregates after interaction with aggrecans.…”

Section: Discussionmentioning

confidence: 99%

Type II collagen-hyaluronan hydrogel – a step towards a scaffold for intervertebral disc tissue engineering

Calderon

Collin²,

Velasco-Bayon³

et al. 2010

eCM

114

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Intervertebral disc regeneration strategies based on stem cell differentiation in combination with the design of functional scaffolds is an attractive approach towards repairing/regenerating the nucleus pulposus. The specific aim of this study was to optimise a composite hydrogel composed of type II collagen and hyaluronic acid (HA) as a carrier for mesenchymal stem cells. Hydrogel stabilisation was achieved by means of 1-ethyl-3(3-dimethyl aminopropyl) carbodiimide (EDC) and Nhydroxysuccinimide (NHS) cross-linking. Optimal hydrogel properties were determined by investigating different concentrations of EDC (8mM, 24mM and 48mM). Stable hydrogels were obtained independent of the concentration of carbodiimide used. The hydrogels crosslinked by the lowest concentration of EDC (8mM) demonstrated high swelling properties. Additionally, improved proliferation of seeded rat mesenchymal stem cells (rMSCs) and hydrogel stability levels in culture were observed with this 8mM cross-linked hydrogel. Results from this study indicate that EDC/NHS (8mM) cross-linked type II collagen/HA hydrogel was capable of supporting viability of rMSCs, and furthermore their differentiation into a chondrogenic lineage. Further investigations should be conducted to determine its potential as scaffold for nucleus pulposus regeneration/repair.

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Determination of collagen-proteoglycan interactions in vitro

Cited by 50 publications

References 39 publications

A Hydrogel-Mineral Composite Scaffold for Osteochondral Interface Tissue Engineering

A Hydrogel-Mineral Composite Scaffold for Osteochondral Interface Tissue Engineering

Photocrosslinkable polysaccharides based on chondroitin sulfate

Type II collagen-hyaluronan hydrogel – a step towards a scaffold for intervertebral disc tissue engineering

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