Determination of cocaine and its major metabolite benzoylecgonine in rabbit hair by GC/MS

Abstract

It is important to determine people who use drugs to prevent the use of them. In recent years, hair is routinely used on toxicological analysis as well as biological samples such as blood and urine. The major practical advantages of hair testing are larger detection windows; non-invasive sample collection; easy to transport and store in addition hair analysis can provide a retrospective calendar of an individual's drug use history. In this study, Male New Zealand rabbits (n=10) weighing 2-2,5 kg were used. Two groups were constituted and each group has five rabbits. New Zealand rabbits were intraperitoneally administered with a single dose of cocaine. Following that hair samples were collected during the12th day. According to our data; cocaine is appealed the first five days after single dose of cocaine administration. Benzoylecgonine was not detected after three days. Frequency of benzoylecgonine existence was lower than cocaine. Limit of Detection (LOD) and Limit of Quantification (LOQ) were calculated for cocaine and benzoylecgonine. We did not any significant between groups.

Anthropogenic contaminants of high concern: Existence in water resources and their adverse effects

“…Benzoylecgonine is the main metabolite of cocaine and an analgesic from the pharmaceutical industry (Efeoglu et al, 2013). Several studies have been reported the existence of Benzoylecgonine in different water bodies, around the globe.…”