Determination of clenbuterol in pork and potable water samples by molecularly imprinted polymer through the use of covalent imprinting method

Tang, Yiwei; Lan, Jianxing; Gao, Xue; Liu, Xiuying; Zhang, Defu; Wei, Liqiao; Gao, Ziyuan; Lǐ, Jiànróng

doi:10.1016/j.foodchem.2015.06.067

Cited by 82 publications

(23 citation statements)

References 28 publications

(33 reference statements)

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“…Therefore, there is a demand for relevant sensitive, selective, fast and inexpensive analytical protocols from legislation, health authorities and companies operating in the food market [15]. Various analytical techniques have been reported for the analysis of FF, including high performance liquid chromatography, gas chromatography, liquid chromatography-Tandem mass spectrometry [16], gas chromatography-Mass spectrometry [17], and enzyme-linked immunosorbent assay (ELISA) [18]. The manufacture of molecular imprinting polymers (MIP) can be very valuable for those selective and sensitive methods that are needed.…”

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confidence: 99%

Florfenicol Binding to Molecularly Imprinted Polymer Nanoparticles in Model and Real Samples

et al. 2020

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A simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop assays similar to the enzyme-linked immunosorbent (ELISA) assay to determine and quantify florfenicol (FF) in real food samples such as liquid milk and salmon muscle is presented here. The nanoMIPs were synthesized by a solid-phase approach with an immobilized FF (template) and characterized using dynamic light scattering, a SPR-2 biosensor system and transmission electron microscopy. Immobilization of nanoMIPs was conducted by preparing a homogenous solution of FF-nanoMIPs in water mixed with polyvinyl alcohol (PVA) 0.2% (w/v) in each well of a microplate. The detection of florfenicol was achieved in competitive binding experiments with a horseradish peroxidase−florfenicol (FF-HRP) conjugate. The assay made it possible to measure FF in buffer and in real samples (liquid milk and salmon muscle) within the range of 60−80 and 90-100 ng/mL, respectively. The immobilized nanoMIPs were stored for six weeks at room temperature and at 5 • C. The results indicate good signal recovery for all FF concentrations in spiked milk samples, without any detrimental effects to their binding properties. The high affinity of nanoMIPs and the lack of a requirement for cold chain logistics make them an attractive alternative to traditional antibodies used in ELISA.with florfenicol (FF) being the main antibiotic used (52%), followed by oxytetracycline, flumequine and oxolinic acid (44%, 1% and 1%, respectively) [5,6]. FF, a fluorinated derivative of thiamphenicol, is a new generation of fenicol drugs [7]. Apart from in the aquaculture industry, FF is widely used as chloramphenicol alternative to prevent and treat bacterial diseases in pigs [8], bovine [9], poultry [10] and animal edible tissues [11]. Several countries adopted maximum permissible residue limits (MRLs) of antibiotics in foods of animal origin, such as meat, milk and eggs [12]. In Chile, the maximum permissible residue limit of FF in muscle and skin of salmon is 1000 µg/kg [13]. At present, no MRL has been set for FF in milk. It is however of vital importance to develop a reliable method for FF analysis in milk products to avoid potential harmful effects to consumers [14]. Therefore, there is a demand for relevant sensitive, selective, fast and inexpensive analytical protocols from legislation, health authorities and companies operating in the food market [15]. Various analytical techniques have been reported for the analysis of FF, including high performance liquid chromatography, gas chromatography, liquid chromatography-Tandem mass spectrometry [16], gas chromatography-Mass spectrometry [17], and enzyme-linked immunosorbent assay (ELISA) [18]. The manufacture of molecular imprinting polymers (MIP) can be very valuable for those selective and sensitive methods that are needed. Molecular impression is a technique for producing in a polymer matrix selective binding sites that recognize a particular molecule. These polymers are very sta...

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Florfenicol Binding to Molecularly Imprinted Polymer Nanoparticles in Model and Real Samples

et al. 2020

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“…Methanol, NaCl, KCl, and Tween-20 were got from Tianjin Chemical Reagent Factory (Tianjin, China). The CLB MIPs were prepared in our laboratory by covalent imprinting method, and the adsorption property of the MIPs was studied and published (Tang et al, 2016). Herein, we used this CLB MIPs as biomimetic antibody to develop a visual flow-through column immunoassay.…”

Section: Chemical and Reagentsmentioning

confidence: 99%

Visual flow-through column biomimetic immunoassay using molecularly imprinted polymer as artificial antibody for rapid detection of clenbuterol in water sample

Tang

Zhang

Zou

et al. 2017

Food and Agricultural Immunology

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A novel visual flow-through column biomimetic immunoassay using horseradish peroxidase-labeled clenbuterol (HRP-CLB) as a tracer and molecularly imprinted polymers (MIPs) with high specificity as the artificial antibody has been developed for rapid detection of CLB. Different intensities of blue color products on test layer of the column could be observed after reaction. This optimized assay provided a preliminary qualitative result in the concentration range of 0 to 1000 μg/L by judging color density of the test layer without any equipment, and the detection could be finished within 15 min. This assay was evaluated using CLB spiked water samples and results were comparable to high performance liquid chromatography method. The visual detection limit of 5 μg/L in water sample was obtained. This developed flow-through column biomimetic immunoassay was demonstrated as a suitable tool for rapid, sensitive, low-cost, and qualitative determination of CLB residues on site.

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“…The concept of molecularly imprinting can be dated back as far as the 1930′s, with the basic principal involving the formation of a polymeric network around a small extractable template molecule to leave a complementary nanocavity was noted [ [18] , [19] , [20] ]. Most commonly, the imprinting process is conducted by introducing the template molecule to functional monomer and functional crosslinking agent, before initiating polymerization by the addition of a photo/thermal initiator and the requisite conditions [ [21] , [22] , [23] , [24] ]. The template can then extracted by means of mechanical grinding and solid phase extraction by prolonged washing with protic solvents [ [25] , [26] , [27] ] ( Fig.…”

Section: Introductionmentioning

confidence: 99%

MIPs for commercial application in low-cost sensors and assays – An overview of the current status quo

Lowdon

Diliën

Singla

et al. 2020

Sensors and Actuators B: Chemical

138

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Highlights The review aims to summarize recent commercially interesting innovations in MIP-based sensor design. In addition to sensors, commercially viable assays based on MIPs are analysed and recent advances are summarized and discussed. The review also analyses how commercial MIP companies could contribute to a next step in the commercialization of MIP-based detection systems. The review contains a critical analysis and discussion on MIP-based sensors and assay commercialization as well as an outlook/recommendation for the future.

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Determination of clenbuterol in pork and potable water samples by molecularly imprinted polymer through the use of covalent imprinting method

Cited by 82 publications

References 28 publications

Florfenicol Binding to Molecularly Imprinted Polymer Nanoparticles in Model and Real Samples

Florfenicol Binding to Molecularly Imprinted Polymer Nanoparticles in Model and Real Samples

Visual flow-through column biomimetic immunoassay using molecularly imprinted polymer as artificial antibody for rapid detection of clenbuterol in water sample

MIPs for commercial application in low-cost sensors and assays – An overview of the current status quo

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