Determination of binding parameters between lysozyme and its aptamer by frontal analysis continuous microchip electrophoresis (FACMCE)

Girardot, Marion; Li, Hong‐Yi; Descroix, Stéphanie; Varenne, Anne

doi:10.1016/j.chroma.2011.04.077

Cited by 21 publications

(14 citation statements)

References 41 publications

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“…It is important to note, that this type of measurement is not unique in microfluidics as there is much precedent in the literature detailing both study and quantification of affinity constants between ligands and antibodies [20][21][22]. For example, Holden and Cremer provide a nice review on recent biophysical experiments to obtain mechanistic details of biomacromolecule interactions in microfluidic devices utilizing only nanoliters of analyte [23].…”

Section: Resultsmentioning

confidence: 97%

Development of microfluidic-based assays to estimate the binding between osteocalcin (BGLAP) and fluorescent antibodies

Carmona

Valadez

Yun

et al. 2015

Talanta

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Section: Resultsmentioning

confidence: 97%

Development of microfluidic-based assays to estimate the binding between osteocalcin (BGLAP) and fluorescent antibodies

Carmona

Valadez

Yun

et al. 2015

Talanta

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“…Some examples are HAPIscreen [38], Emulsion PCR [39], primer-free SELEX [40] or FACMCE [41]. Basically, the goal is now to obtain several aptamers for different targets reducing times of selection and characterization by combining techniques such as massively parallel sequencing and microfluidics (Multiplexed SELEX) [42].…”

Section: Updating Selex Methods With Modern Technologies (2004–2011)mentioning

confidence: 99%

RNA Aptamer Evolution: Two Decades of SELEction

Aquino‐Jarquín

Toscano-Garibay

2011

IJMS

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Aptamers are small non-coding RNAs capable of recognizing, with high specificity and affinity, a wide variety of molecules in a manner that resembles antibodies. This class of nucleic acids is the resulting product of applying a well-established screening method known as SELEX. First developed in 1990, the SELEX process has become a powerful tool to select structured oligonucleotides for the recognition of targets, starting with small molecules, going through protein complexes until whole cells. SELEX has also evolved along with new technologies positioning itself as an alternative in the design of a new class of therapeutic agents in modern molecular medicine. This review is an historical follow-up of SELEX method over the two decades since its first appearance.

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“…The obtained dissociation constant agreed with the values published in the literature, but the determined number of binding sites indicated that the BGE composition and systematic evolution of the ligand by an exponential enrichment process influenced the number and affinity of the binding sites. By using mathematical linearization methods, the results showed two sites with different binding affinities . The applications of the MC‐CE method for affinity studies are summarized in Table .…”

Section: Microchip Capillary Electrophoresismentioning

confidence: 99%

Capillary electrophoresis‐based approaches for the study of affinity interactions combined with various sensitive and nontraditional detection techniques

2019

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Nearly all processes in living organisms are controlled and regulated by the synergy of many biomolecule interactions involving proteins, peptides, nucleic acids, nucleotides, saccharides, and small molecular weight ligands. There is growing interest in understanding them, not only for the purposes of interactomics as an essential part of system biology, but also in their further elucidation in disease pathology, diagnostics, and treatment. The necessity of detailed investigation of these interactions leads to the requirement of laboratory methods characterized by high efficiency and sensitivity. As a result, many instrumental approaches differing in their fundamental principles have been developed, including those based on capillary electrophoresis. Although capillary electrophoresis offers numerous advantages for such studies, it still has one serious limitation, its poor concentration sensitivity with the most commonly used detection method–ultraviolet‐visible spectrometry. However, coupling capillary electrophoresis with a more sensitive detector fulfils the above‐mentioned requirement. In this review, capillary electrophoresis combined with fluorescence, mass spectrometry, and several nontraditional detection techniques in affinity interaction studies are summarized and discussed, together with the possibility of conducting these measurements in microchip format.

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Determination of binding parameters between lysozyme and its aptamer by frontal analysis continuous microchip electrophoresis (FACMCE)

Cited by 21 publications

References 41 publications

Development of microfluidic-based assays to estimate the binding between osteocalcin (BGLAP) and fluorescent antibodies

Development of microfluidic-based assays to estimate the binding between osteocalcin (BGLAP) and fluorescent antibodies

RNA Aptamer Evolution: Two Decades of SELEction

Capillary electrophoresis‐based approaches for the study of affinity interactions combined with various sensitive and nontraditional detection techniques

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