Determination of amino acids by capillary electrophoresis with differential resonant contactless conductivity detector

Shen, Dazhong; Li, Yaolong; Zhang, Zhenli; Zhang, Ping; Kang, Qi

doi:10.1016/j.talanta.2012.11.027

Cited by 19 publications

(24 citation statements)

References 43 publications

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“…This confirms earlier studies with a different cell in which the capacitances were experimentally determined differently, namely from Bode plots (plots of signal vs. frequency) . Shen and co‐workers also studied in detail the effect of solution conductivity on wall capacitance and found a clear correlation, that is, the wall capacitance was higher for solutions of higher conductivity . The authors proposed a model for a likely explanation, namely that the field lines between the electrodes are following different paths for materials of different conductivity inside the tubing.…”

Section: Fundamental Aspectssupporting

confidence: 75%

“…together with a simplified equivalent circuit diagram. Shen and co‐workers carried out fundamental studies of this standard cell configuration with an impedance analyzer, and found that the measured wall capacitances are significantly smaller than the values calculated from the formula for a coaxial capacitor. This confirms earlier studies with a different cell in which the capacitances were experimentally determined differently, namely from Bode plots (plots of signal vs. frequency) .…”

Section: Fundamental Aspectsmentioning

confidence: 93%

See 1 more Smart Citation

Contactless conductivity detection for analytical techniques—Developments from 2012 to 2014

Kubáň

Hauser

2014

Electrophoresis

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The review covers the progress of capacitively coupled contactless conductivity detection over the 2 years leading up to mid-2014. During this period many new applications for conventional CE as well as for microchip separation devices have been reported; prominent areas have been clinical, pharmaceutical, forensic, and food analyses. Further progress has been made in the development of field portable instrumentation based on CE with contactless conductivity detection. Several reports concern the combination with sample pretreatment techniques, in particular electrodriven extractions. Accounts of arrays of contactless conductivity detectors have appeared, which have been created for quite different tasks requiring spatially resolved information. The trend of the use of contactless conductivity measurements for applications other than CE has continued.

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Section: Fundamental Aspectssupporting

confidence: 75%

Section: Fundamental Aspectsmentioning

confidence: 93%

Contactless conductivity detection for analytical techniques—Developments from 2012 to 2014

Kubáň

Hauser

2014

Electrophoresis

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“…Shen et al. presented a similar arrangement employing two detector cells for a conventional capillary, but used a very special circuitry which included two resonating quartz crystals 27. The arrangement for conventional capillaries reported herein employs a straightforward standard circuitry and a common cell.…”

Section: Introductionmentioning

confidence: 99%

Referenced Capacitively Coupled Conductivity Detector for Capillary Electrophoresis

2013

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A dual capacitively coupled contactless conductivity detector for capillary electrophoresis was developed. The two channels are arranged in a bridge configuration so that one of them acts as a reference whose signal is subtracted. This effectively compensates for the baseline conductivity of the separation buffer so that the electronic zero setting is not necessary. Changes in the buffer composition are automatically accounted for, as are temperature drifts. The system is demonstrated for the detection of inorganic model cations in capillary electrophoresis. Besides the use with two separate capillaries, one of which solely serves as reference, it was also found possible to use a single capillary which is looped back through the reference cell.

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“…Although disposable electrodes may be a solution, they are costly and inconvenient. 29,30 To date, several studies have been reported to monitor reactions between antigen and antibody in real time without using a labeled compound by an amperometric technique. 24 To overcome these disadvantages, we developed an electrochemical platform which was based on an integrated and contact-free microuidic chip for label-free and real-time impedance monitoring of isothermal nucleic acid amplication directly.…”

Section: Introductionmentioning

confidence: 99%

Real-time determination of carcinoembryonic antigen by using a contactless electrochemical immunosensor

et al. 2016

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A sensitive label-free and contactless impedance immunosensor is developed for the real-time interaction investigation between a cancer biomarker carcinoembryonic antigen (CEA) and its antibody CEA 5909. The immunosensor consists of one interdigitated electrode (IDE) based contactless microfluidic chip and a supplementary inductor. The thin insulation layer between the IDEs and the resolved solution makes the detection contactless, preventing electrode erosion, electrolysis, and bubble formation problems. The insulated IDEs are covered by a PDMS layer with a microchamber of 5 mL. The leakage capacitance of the microfluidic chip is compensated by the addition of a series inductor. Under resonant characterization conditions, reproducible determination of ionic solutions in phosphate-buffered saline (PBS) has been demonstrated, with a detection limit of 10 mM. The immunoreactions between antibody CEA 5909 and CEA, a cytokeratin 19 fragment (CYFRA21-1) and bovine serum albumin (BSA) wereinvestigated under label and label-free conditions. The immunosensor is proved to have a specific binding process between CEA and antibody CEA 5909 with a detection limit as low as 100 fg mL À1 in PBS within a few minutes.

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Determination of amino acids by capillary electrophoresis with differential resonant contactless conductivity detector

Cited by 19 publications

References 43 publications

Contactless conductivity detection for analytical techniques—Developments from 2012 to 2014

Contactless conductivity detection for analytical techniques—Developments from 2012 to 2014

Referenced Capacitively Coupled Conductivity Detector for Capillary Electrophoresis

Real-time determination of carcinoembryonic antigen by using a contactless electrochemical immunosensor

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