Determination of 12 commonly found compounds in DUID cases in whole blood using fully automated supported liquid extraction and UHPLC-MS/MS

Kristoffersen, Lena; Langødegård, Marit; Gaare, Kristin Irene; Amundsen, I.; Terland, M.N.; Strand, Dag Helge

doi:10.1016/j.jchromb.2018.06.050

Cited by 31 publications

(20 citation statements)

References 38 publications

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“…Not only does automation minimise sample handling and error, it also increases the throughput of analysis, with the ability to prepare multiple 96 well plates per day [28]. SLE has been adopted as a method of sample clean up in toxicology such as the screening of drugs of abuse in urine [29] and blood [30]. It has been used for sample preparation of serum (100 µL) prior to derivatisation and GC-MS steroid profiling in mouse [31].…”

Section: Discussionmentioning

confidence: 99%

Automated Supported Liquid Extraction for the analysis of a panel of 12 endogenous steroids in human plasma by LC-MS/MS

Denham¹,

Just²,

Kyle³

et al. 2020

Preprint

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Steroid analysis is important in the clinical assessment of endocrine function in health and disease. Although tandem mass spectrometry methods coupled with chromatographic separation are considered the gold standard analytical technique in this setting, enabling profiling of multiple steroids in a single sample, sample processing can be labour-intensive. Here we present a simple, efficient automated 96-well Supported Liquid Extraction method with dichloromethane/isopropanol as organic solvent, carried out on a Extrahera automated sample handler (Biotage), which completes sample preparation of 80 plasma samples (200µL) in 90 minutes. Compounds were separated on a Kinetex C18 column (150x3mm;2.6um) using a mobile phase of methanol and water (0.1% formic acid). The run time was 16 minutes on a Nexera uHPLC system (Shimadzu) with a QTrap 6500+ linear ion trap mass spectrometer (AB Sciex). Precisions ranged 8.1 to 18.1% RSD, bias -10.1-5.8%, and extraction recoveries 73.5-111.9%. LOQs ranged between 0.025–0.500 ng/mL.

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Section: Discussionmentioning

confidence: 99%

Automated Supported Liquid Extraction for the analysis of a panel of 12 endogenous steroids in human plasma by LC-MS/MS

Denham¹,

Just²,

Kyle³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…This is the same sample preparation and LC-MS/MS method used by Kristoffersen et al . 20 Stable isotope-labelled internal standards were used for all compounds. Two multiple reaction monitor (MRM) transitions were used for each compound and each internal standard.…”

Section: Methodsmentioning

confidence: 99%

“…We prepared the samples by 96-well supported liquid extraction, which were then analysed by liquid chromatography-tandem mass spectrometry (LC-MS/ MS). This is the same sample preparation and LC-MS/ MS method used by Kristoffersen et al 20 Stable isotopelabelled internal standards were used for all compounds. Two multiple reaction monitor (MRM) transitions were used for each compound and each internal standard.…”

Section: Blood Analysismentioning

confidence: 99%

Prevalence of psychoactive substance use among acutely hospitalised patients in Oslo and Moscow: a cross-sectional, observational study

Gamboa

Jørgenrud

Bryun³

et al. 2020

BMJ Open

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ObjectivesThe use of psychoactive prescription medication is increasing in the general population. This is a cause for concern, particularly among the elderly, where physiological changes related to senescence increase the risk for adverse effects. While previous studies regarding psychoactive substance use have generally been population based, we sought to determine the frequency of such use among acutely hospitalised patients.SettingTwo emergency departments (EDs), one in Oslo and one in Moscow, admitting patients to Departments of Internal Medicine.Participants5583 patients aged ≥18 years participated, distributed evenly between genders and study locations. Patients unable to give informed consent were excluded. The study sites did not admit patients with surgical conditions and/or injuries.Primary and secondary outcomesThe presence of psychoactive substances was determined through blood analysis using liquid chromatography-mass spectrometry. Secondary outcomes comprised demographic data (including age, gender, employment and marital status), degree of psychological distress, concurrent alcohol use, and self-reported alcohol, psychoactive drug and illicit substance use.Results32.3% in Oslo and 12% in Moscow were positive for one or more psychoactive medicinal drugs (benzodiazepines, z-hypnotics, opioids or barbiturates). In Oslo, medicinal drug use was associated with being aged 61 to 70 years (OR 2.40, 95% CI 1.61 to 3.58) compared with 18 to 40 years, and psychological distress (OR 2.61, 95% CI 2.06 to 3.30). In Moscow, psychoactive medicinal drug use was also associated with psychological distress (OR 1.68, 95% CI 1.18 to 2.39), and was less common among patients aged 41 to 60 years (OR 0.62, 95% CI 0.43 to 0.88) than among patients aged 18 to 40 years.ConclusionA significant proportion of admitted patients used one or more psychoactive medicinal drugs, in particular benzodiazepines (Oslo and Moscow) and opiates (Oslo). We suggest formalised screening for inappropriate prescription drug use and increased adherence to clinical prescription guidelines.

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“…Concentrations of methadone and buprenorphine in samples of whole blood were determined using ultra‐high‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) after 96‐well supported liquid extraction , which was slightly modified for the determination of methadone and buprenorphine by adding relevant calibration standards. The cut‐off concentrations were 0.5 nM for methadone and 0.2 nM for buprenorphine.…”

Section: Methodsmentioning

confidence: 99%

A clinical trial on the acute effects of methadone and buprenorphine on actual driving and cognitive function of healthy volunteers

Strand

Vindenes

Gjerde

et al. 2018

Brit J Clinical Pharma

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AimsThe present study assessed the acute effects of methadone and buprenorphine on actual on‐road driving performance and neurocognitive function.MethodsMethadone (5 and 10 mg per os) and buprenorphine (0.2 and 0.4 mg sublingual) were administered to 22 healthy volunteers in a five‐way, double‐blind, randomized, placebo‐controlled, double‐dummy, cross‐over study. Driving performance was assessed with an on‐road driving test. The primary outcome measure was standard deviation of lateral position (SDLP), a measure of road tracking control. Laboratory tests were used to measure cognitive function (e.g. reaction time and attention) and questionnaires were used to assess subjective measures of mood and sedation.ResultsThere was no significant main effect of treatment on SDLP. Yet, analysis of individual drug‐placebo contrast data revealed that buprenorphine 0.4 mg significantly increased SDLP. Driving impairment was mild and below the impairment threshold of a blood alcohol concentration of 0.5 mg ml−1. Four participants stopped their driving test while under the influence of either opioid due to sleepiness. Both opioids produced impairments of cognitive task performance and increased sleepiness particularly at the highest dose.ConclusionsAnalgesic doses of buprenorphine produced mild impairing effects on driving and related cognitive skills, while methadone impaired cognitive task performance but not driving performance. Large individual variations were observed for both drugs. Patients should be informed about the possibility of driving impairment when initiating opioid treatment.

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Determination of 12 commonly found compounds in DUID cases in whole blood using fully automated supported liquid extraction and UHPLC-MS/MS

Cited by 31 publications

References 38 publications

Automated Supported Liquid Extraction for the analysis of a panel of 12 endogenous steroids in human plasma by LC-MS/MS

Automated Supported Liquid Extraction for the analysis of a panel of 12 endogenous steroids in human plasma by LC-MS/MS

Prevalence of psychoactive substance use among acutely hospitalised patients in Oslo and Moscow: a cross-sectional, observational study

A clinical trial on the acute effects of methadone and buprenorphine on actual driving and cognitive function of healthy volunteers

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