Determination and pharmacokinetic study of four lignans in rat plasma after oral administration of an extract of Valeriana amurensis by ultra‐high performance liquid chromatography with tandem mass spectrometry

Abstract

A selective and sensitive ultra-high performance liquid chromatography with tandem mass spectrometry method was developed and validated for the determination and pharmacokinetic study of (+)-8-hydroxypinoresinol-4'-O-β -D-glucopyranoside, prinsepiol-4-O-β-D-glucopyranoside, (+)-pinoresinol-4,4'-di-O-β-D-glucopyranoside, and (-)-massoniresinol 3α-O-β-D-glucopyranoside in rat plasma after the oral administration of a Valeriana amurensis extract. The analytes and ethyl 4-hydroxybenzoate (internal standard) were separated on a Waters ACQUITY UPLC HSS T3 chromatographic column. The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode using an electrospray ionization source operating in negative ionization mode. The linear ranges (ng/mL) of the standard curves were 0.39-154.00, 0.62-244.70, 0.50-198.60, and 0.34-134.50 for (+)-8-hydroxypinoresinol-4'-O-β-D-glucopyranoside, prinsepiol-4-O-β-D-glucopyranoside, (+)-pinoresinol-4,4'-di-O-β-D-glucopyranoside, and (-)-massoniresinol 3α-O-β-D-glucopyranoside, respectively. The inter- and intra-day precisions were less than 11.0%, the accuracies were between -5.9 and 7.7%, and the extraction recoveries of the four analytes were > 81.2% from rat plasma. The method was successfully applied to a pharmacokinetic study of the four analytes after oral administration of a Valeriana amurensis extract to rats. The developed method has the potential for pharmacokinetic analysis and to provide additional information in the clinical application of Valeriana amurensis.

Ultra high performance liquid chromatography–electrospray ionization‐tandem mass spectrometry and pharmacokinetic analysis of justicidin B and 6′‐hydroxy justicidin C in rats

“…Heparinized blood was centrifuged at 13 000 × g at 18°C for 5 min to obtain plasma. Plasma samples were then removed and stored at −80°C until analysis .…”

Simultaneous quantification of triterpenoid saponins in rat plasma by UHPLC–MS/MS and its application to a pharmacokinetic study after oral total saponin of Aralia elata leaves

“…The result indicated that the absorption of four triterpenoid saponins might be slow, and the minimum molecular masses of the analytes are 957.5 Da, larger than the favorable value (<500 Da). Based on the above situation, the low absorption may be due to poor membrane permeability [21,22]. The T max of the four analytes was 0.83-1.58 h and t 1/2 was 4.02-5.29 h, the above results indicated that the compounds are rapidly absorbed in rats, quickly eliminated, and unlikely to accumulate in vivo.…”

Characterizing Alzheimer's disease through metabolomics and investigating anti‐Alzheimer's disease effects of natural products

“…It contains numerous components, such as monoterpenes, sesquiterpenes, iridoids, lignans, alkaloids, and flavonoids . Furthermore, V. amurensis extracts have hypnotic, antidepressive, antispasmodic, and sedative effects . UPLC‐MS–based metabolomics were performed to investigate the plasma metabolic profiles and potential biomarkers in Aβ 25–35 ‐induced AD mice after treatment with V. amurensis extracts.…”

“…Valeriana amurensis is a traditional medicinal plant from the Valerianaceae family that is extensively used in folk medicine for the treatment of neurasthenia and insomnia. It contains numerous components, such as monoterpenes, sesquiterpenes, iridoids, lignans, alkaloids, and flavonoids . Furthermore, V. amurensis extracts have hypnotic, antidepressive, antispasmodic, and sedative effects .…”

“…50 Furthermore, V. amurensis extracts have hypnotic, antidepressive, antispasmodic, and sedative effects. 50 UPLC-MS-based metabolomics were performed to investigate the plasma metabolic profiles and potential biomarkers in A␤ [25][26][27][28][29][30][31][32][33][34][35] -induced AD mice after treatment with V. amurensis extracts. The results indicated that V. amurensis extracts can gradually restore all the potential biomarkers close to normal states by regulating the amino acids, phospholipids, and sheath lipid metabolism in the AD mice: l-histidine, C18 PHS, hexadecasphinganine, LPC C22:6, LPC C18:2, LPC C16:0, LPC C20:4, LPC C18:1, dihydrosphingosine, and C20 PHS.…”