Determinants of Genital Human Papillomavirus Infection Among Cytologically Normal Women Attending the University of New Mexico Student Health Center

Wheeler, Cosette M.; Parmenter, Cheryl A.; Hunt, William C.; Becker, Thomas M.; Greer, Catherine E.; Hildesheim, Allan; Manos, M. Michele

doi:10.1097/00007435-199309000-00009

Cited by 150 publications

(98 citation statements)

References 9 publications

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“…These data differ greatly from reports of HPV infection in women, in whom oncogenic HPV types, specifically type 16, are more common [31][32][33][34]. In some previous studies on male HPV, the majority of men who tested positive for HPV had oncogenic subtypes [10,12,30], but, in the IARC 5-nation study, unspecified HPV types were most common [16].…”

Section: Discussioncontrasting

confidence: 80%

Human Papillomavirus Infection in Men Attending a Sexually Transmitted Disease Clinic

et al. 2003

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Human papillomavirus (HPV) is the main etiologic agent of anogenital cancers, including cervical cancer, but little is known about the type-specific prevalence of HPV in men. Participants were men aged 18-70 years attending a sexually transmitted disease clinic. Penile skin swabs were assessed for HPV DNA using polymerase chain reaction with reverse line-blot genotyping. Of 436 swabs collected, 90.1% yielded sufficient DNA for HPV analysis. Men with inadequate swab samples were significantly more likely to be white and circumcised than men with adequate swab samples. The prevalence of HPV was 28.2%. Oncogenic HPV types were found in 12.0% of participants, nononcogenic types were found in 14.8% of participants, multiple types were found in 6.1% of participants, and unknown types were found in 5.9% of participants. The most prevalent subtypes were nononcogenic 6, 53, and 84. HPV positivity was not associated with age. These results indicate that HPV infection among men at high risk is common but that characteristics of male HPV infection may differ from those of female infection.Human papillomavirus (HPV) infection is the necessary, sexually transmitted cause of invasive cervical cancer and its precursor lesion, cervical intraepithelial neoplasia [1][2][3]. HPV has also been closely linked with other anogenital cancers, including anal cancer and certain penile cancers [4][5][6]. HPV infection in men is over- whelmingly subclinical, which has resulted in a potentially large number of asymptomatic carriers who serve as reservoirs and vectors for the virus.Although HPV has been studied extensively in women, data on male infection are limited. Studies of HPV in men are necessary to improve our understanding of HPV transmission and HPV-related carcinogenesis and to prevent disease in both men and women. The success of future cancer prevention strategies, such as prophylactic HPV vaccination, will be limited without a basic epidemiological understanding of HPV in men.Earlier studies of papillomavirus infection in men used a variety of clinical and histological techniques to establish a diagnosis of HPV, but polymerase chain reaction (PCR) has emerged as the most sensitive method available for the detection of latent HPV [7][8][9]. Among studies that have used PCR to detect penile HPV DNA in healthy men, sampling methodologies have been inconsistent. Nonetheless, results from these diverse investigations have suggested that penile HPV in sexually active men is at least as prevalent as cervical HPV is among women [10][11][12][13][14][15].HPV prevalence in men has been shown to vary by

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Section: Discussioncontrasting

confidence: 80%

Human Papillomavirus Infection in Men Attending a Sexually Transmitted Disease Clinic

et al. 2003

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“…This was synthesized with multiple nucleotides in each primer and mixed with 25 primers including HMB01M, which detect a large spectrum of HPV types? This has been used in North and South America as well as in Asia (Wheeler et al, 1993;Hildesheim et al, 1994;Ley et al, 1994;Liaw et al, 1995;Lazcano et al, 2001;Thomas et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Human Papillomavirus in Women from Saudi Arabia

Turki¹,

Sait²,

Anfinan³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Human papillomavirus (HPV) infection is the main causes of cervical cancer in women worldwide. The goal of the present study was to determine the prevalence and distribution of HPV genotypes in women from Saudi Arabia. Recently, several HPV detection methods have been developed, each with different sensitivities and specificities. Methods: In this study, total forty cervical samples were subjected to polymerase chain reaction and hybridization to BioFilmChip microarray assessment. Results: Human papillomavirus (HPV) infections were found in 43% of the specimens. The most prevalent genotypes were HPV 16 (30%) HPV 18 (8.0%) followed by type HPV 45, occurring at 5.0%. Conclusion: Our finding showed the HPV infection and prevalence is increasing at alarming rate in women of Saudi Arabia. There was no low risk infection detected in the tested samples. The BioFilmChip microarray detection system is highly accurate and suitable for detection of single and multiple infections, allowing rapid detection with less time-consumption and easier performance as compared with other methods.

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“…1 Laboratory and epidemiological research has provided overwhelming evidence that some subtypes of human papillomavirus (HPV) are a ''necessary but insufficient cause'' for cervical cancer. [2][3][4][5] Therefore, other risk factors are also needed for the initiation and promotion of cervical carcinogenesis.…”

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confidence: 99%

N7‐methyldeoxyguanosine levels in DNA isolated from cervical cytology samples are associated with smoking

Harrison

Khan

Dey

et al. 2006

Intl Journal of Cancer

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Smoking has been associated, in epidemiological studies, with an increased risk of cervical neoplasia. This may be in part due to the presence of tobacco-specific nitrosamines in cervical mucous of smokers, which may result in carcinogenic DNA damage. We have thus examined whether cervical DNA contains alkylation damage arising from exposure to methylating agents (N7-methyldeoxyguanosine, N7-MedG). DNA was extracted from cervical cytology samples and N7-MedG levels were measured using an immunoslotblot assay. Ninety percentage of the DNA samples were alkylated and N7-MedG levels (mean, 95% CI) in ever-smokers (1.27, 0.90-1.81 lmol/mol dG) were significantly higher than those in nonsmokers (0.42, 0.20-0.91 lmol/mol dG: p 5 0.005). N7-MedG adduct levels were significantly correlated with number of cigarettes smoked per day and pack years of cigarette smoking in current smokers. There was no association with N7-MedG levels and cervical intraepithelial neoplasia status, age, parity or contraception use. Our study suggests that cervical DNA contains alkylation damage that can arise from exposure to cigarette smoke. ' 2006 Wiley-Liss, Inc.Key words: alkylation; smoking; cervical cancer; N7-methylguanine Cervical cancer is one of the most common neoplastic diseases affecting women with a combined worldwide incidence of almost half a million new cases annually.1 Laboratory and epidemiological research has provided overwhelming evidence that some subtypes of human papillomavirus (HPV) are a ''necessary but insufficient cause'' for cervical cancer.2-5 Therefore, other risk factors are also needed for the initiation and promotion of cervical carcinogenesis.A number of epidemiological studies suggest that cigarette smoking may be an aetiological risk factor for cervical cancer. 6-10The risk for neoplasia appears to increase with longer duration and intensity of smoking. 11,12 The cervix is known to contain enzymes that can metabolise the carcinogens that are present in cigarette smoke, 13,14 and expression of these enzymes may be modified by HPV infection. 14,15 Cigarette-derived chemicals, nicotine and its major metabolite cotinine, have been found in cervical mucus at levels higher than those present in blood serum, [16][17][18][19] and the tobacco-specific nitrosamine 4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone (NNK) is more prevalent in cervical mucus samples from smokers than those from nonsmokers.20 NNK alkylates DNA forming a range of DNA adducts including N7-methyldeoxyguanosine (N7-MedG), a poorly repaired adduct that can act as a biomarker of exposure to alkylating agents. 21 DNA adducts have been detected in human cervical tissues. [22][23][24][25][26][27] In most, but not all, of these studies, adduct levels were higher in smokers with the detected adducts, probably arising from exposure to polycyclic aromatic hydrocarbons present in cigarette smoke. However, other DNA adducts, in particular alkylation-induced lesions, may also be present, but would not have been detected by the procedures used. Our recent r...

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Determinants of Genital Human Papillomavirus Infection Among Cytologically Normal Women Attending the University of New Mexico Student Health Center

Cited by 150 publications

References 9 publications

Human Papillomavirus Infection in Men Attending a Sexually Transmitted Disease Clinic

Human Papillomavirus Infection in Men Attending a Sexually Transmitted Disease Clinic

Prevalence of Human Papillomavirus in Women from Saudi Arabia

N7‐methyldeoxyguanosine levels in DNA isolated from cervical cytology samples are associated with smoking

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