The study examined the effect of electronic banking on the profitability of deposit money Banks in Nigeria. Banking industry is confronted by a new innovation imposed by technology transformation that has changed the way banks conduct their activities from traditional means to electronic means. Furthermore, this study investigates how the electronic banking channels through internet, mobile, POS and ATM have impacted on deposit money banks profitability. It is against this background that the study examined the effect of electronic banking on the profitability of deposit money Banks in Nigeria. The methodology of the study was quantitative in nature. Using time series data of 2006-2015, the study employed regression technique in the analysis of sourced data. Data were collected from secondary sources through annual reports of deposit money Banks in Nigeria. Electronic banking was measured using the total values of internet banking, mobile banking, POS and ATM while Profitability was measured using profit margin of deposit money banks in Nigeria. Profitability was regressed on internet banking, mobile banking, POS and ATM using ordinary least square regression technique. The study revealed that positive relationship exists between mobile banking and Profitability, and between ATM and Profitability. In addition, there is also a statistically significant relationship between POS and Profitability while there is no significant relationship between internet banking and Profitability. As such, E-banking has a positive effect on Profitability of deposit money banks in Nigeria. It is therefore, recommended that banks must offer numerous services through internet banking and effective transaction through the POS Terminals.