Determinants of ADP-induced platelet aggregation in diabetes mellitus

Jones, David B.; Davis, Timothy M. E.; Bown, E.; Carter, R. D.; Mann, Jim; Prescott, R. J.

doi:10.1007/bf00452065

Cited by 24 publications

(15 citation statements)

References 26 publications

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“…Increased sensitivity of platelets from diabetic patients to ADP-, PAF-, and AA-induced aggregation compared with healthy subjects has been reported. [31][32][33] The effect of glycemic control on platelet aggregation has also been documented, suggesting that improvement in glycemic control is followed by reduced sensitivity of platelets to aggregation. 32,34 Our results demonstrate that diet composition can influence the aggregatory response of platelets.…”

Section: Discussionmentioning

confidence: 96%

“…[31][32][33] The effect of glycemic control on platelet aggregation has also been documented, suggesting that improvement in glycemic control is followed by reduced sensitivity of platelets to aggregation. 32,34 Our results demonstrate that diet composition can influence the aggregatory response of platelets. Specifically, the EC 50 values of PAF and ADP were higher in both healthy subjects and T2DM patients after the 1-month consumption of traditional Greek diet, indicating that their platelet function had improved.…”

Section: Discussionmentioning

confidence: 96%

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Effect of Traditional Greek Mediterranean Meals on Platelet Aggregation in Normal Subjects and in Patients with Type 2 Diabetes Mellitus

Antonopoulou

Fragopoulou

Karantonis

et al. 2006

Journal of Medicinal Food

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Patients with type 2 diabetes mellitus have increased risk of cardiovascular disease. Epidemiological studies have shown a correlation between diet and incidence of coronary heart disease. The aim of the study is to determine the effect of a traditional Greek Mediterranean diet on platelet aggregation induced by ADP, arachidonic acid (AA), and especially platelet-activating factor (PAF) on patients with type 2 diabetes mellitus as well as on healthy volunteers. The patients were randomized into two subgroups, A and B. The lipid extracts from traditional Greek Mediterranean-type meals were tested in in vivo for their ability to reduce PAF- or thrombin-induced platelet aggregation. The meals with the most potent anti-aggregating activity were chosen for the diet of both subgroup A and healthy subjects and consumed for a period of 28 days, whereas subgroup B kept to their regular diet that was followed before entering the study. Platelet-rich plasma was isolated before and after the diet, and the ability of platelets to aggregate under the aggregating factors was tested. One-month consumption of diet resulted in a significant reduction in PAF- and ADP-induced aggregation of platelets in both groups of healthy volunteers (PAF and ADP, P < .05) and subgroup A (PAF, P < .001; ADP, P < .05), whereas the AA-induced aggregation was not affected. No effect was observed in subgroup B, which followed the standard diet. Thus the consumption of a traditional Greek Mediterranean diet even for a short period can reduce platelet activity in patients suffering from type 2 diabetes mellitus and in healthy subjects.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

Effect of Traditional Greek Mediterranean Meals on Platelet Aggregation in Normal Subjects and in Patients with Type 2 Diabetes Mellitus

Antonopoulou

Fragopoulou

Karantonis

et al. 2006

Journal of Medicinal Food

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“…Activated phospholipase C causes rapid hydrolysis of phosphoinositides and consequently produces inositol phosphates and diacylglycerol (7,8). It has been documented that platelet aggregability is positively correlated with fasting blood glucose level (2,24). These findings were coupled with significantly enhanced accumulation of PA, which may be generated from diacylglycerol by diacylglycerol kinase (22,23).…”

Section: Discussionmentioning

confidence: 99%

Changes in Phosphoinositide Turnover, Ca2+ Mobilization, and Protein Phosphorylation in Platelets From NIDDM Patients

et al. 1990

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Enhanced platelet functions have been demonstrated in patients with non-insulin-dependent diabetes mellitus (NIDDM). This study evaluated abnormalities in platelet signal transduction in diabetic patients, including turnover of phosphoinositides, mobilization of intracellular Ca2+, and phosphorylation of 20,000- and 47,000-Mr proteins (P20 and P47). Washed platelets were obtained from 6 patients with NIDDM whose platelet aggregation rates were abnormally elevated (DM-A group), 11 NIDDM patients with normal platelet aggregation rates (DM-B group), and 8 age-matched healthy control subjects. The mass and specific radioactivity of phosphatidylinositol 4,5-bisphosphate (PIP2), phosphatidylinositol 4-phosphate (PIP), phosphatidylinositol (PI), and phosphatidic acid (PA) in 32P-labeled platelets were not different among the three groups. Hydrolysis of PIP2, PIP, and PI; accumulation of PA; and phosphorylation of P20 in platelets stimulated by 0.05 U/ml thrombin were significantly increased in the DM-A group compared with the control or DM-B group. There was no difference in P47 phosphorylation among the three groups. On the contrary, P20 and P47 phosphorylation induced by 50 nM of 12-O-tetradecanoylphorbol-13-acetate, an activator of protein kinase C, was significantly decreased in the DM-A group. Additionally, the intracellular free Ca2+ concentration [( Ca2+]i) was measured with the fluorescent Ca2+ indicator fura 2. Although the basal [Ca2+]i value was similar in the three groups, the rise in [Ca2+]i induced by 0.05 U/ml thrombin in the presence and the absence of extracellular Ca2+ was significantly higher in the DM-A group than the other groups.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…26 Of interest in the present study is that ADP-induced platelet aggregation was positively and independently correlated with blood glucose, a finding also reported for diabetes. 27 It has also been shown that platelets from diabetic patients are hypersensitive to agonists such as ADP 28 and are resistant to the inhibitory effect of aspirin, 28 as also described in diabetic animals 29 and HTRs. 30 Another similarity between diabetic and transplanted patients is their tendency to develop intramyocardial small-vessel disease, as shown by pathological examination 1 and coronarography.…”

Section: Platelet Aggregation Relation To Other Clinical and Biologicmentioning

confidence: 94%

Platelet function and composition in heart transplant recipients compared with nontransplanted coronary patients.

Lorgeril

Dureau

Boissonnat

et al. 1992

Arterioscler Thromb

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Accelerated coronary artery disease seems to be the main condition limiting long-term survival after heart transplantation. Ninety-one heart transplant recipients were compared with 94 nontransplanted coronary artery disease patients in an attempt to identify the factors responsible for the accelerated form of coronary artery disease occurring after heart transplantation. Among the parameters examined, heart transplant recipients exhibited a higher plasma level of insulin (8.5±0.5 versus 62±03 mIU/1, p=0.002), a lower plasma level of vitamin E (14.8±0.4 versus 16.9±0.7 mg/1, p=0.03), a higher platelet cholesterol-to-phospholipid ratio (8.9±0J versus 7.6±0«3, p=0.007), and an increased response to ADP-induced platelet aggregation (for the first wave, 29.1±0.9% of maximal aggregation versus 25.1±1.0%, />=0.002; for the second wave, 21.4±1.4% versus 15.9±1.1%, p=0.002, after adjustment for hematocrit), but no untoward changes in the level of fibrinogen, plasminogen activator inhibitor-1, antithrombin III, or lipoprotein(a). In addition, platelet aggregation in patients who required retransplantation as a result of severe coronary artery disease was similar before and after retransplantation. This suggests that severe coronary artery disease is not the cause of platelet hyperaggregability. In multiple-regression analysis, ADP-induced platelet aggregation in heart transplant recipients was significantly positively related to blood glucose (r=0.50, p<0Ml) and inversely related to n-3 fatty acids from platelet phospholipids (r=0.40,p<0.01). These results are consistent with the view that a platelet disorder associated with or responsible for the increased thrombotic tendency could play a role in the pathogenesis of the disease. However, further studies are needed to investigate the mechanism of this platelet hyperaggregability, which is possibly related to a lipid peroxidation process (low plasma vitamin E) and/or to a diabetes-associated platelet lipid disorder (high insulin level). (Arteriosclerosis and Thrombosis 1992;12:222-230)

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Determinants of ADP-induced platelet aggregation in diabetes mellitus

Cited by 24 publications

References 26 publications

Effect of Traditional Greek Mediterranean Meals on Platelet Aggregation in Normal Subjects and in Patients with Type 2 Diabetes Mellitus

Effect of Traditional Greek Mediterranean Meals on Platelet Aggregation in Normal Subjects and in Patients with Type 2 Diabetes Mellitus

Changes in Phosphoinositide Turnover, Ca2+ Mobilization, and Protein Phosphorylation in Platelets From NIDDM Patients

Platelet function and composition in heart transplant recipients compared with nontransplanted coronary patients.

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