Deteksi Virus Utama Bawang Merah dan Bawang Putih dari Daerah Jawa Barat dan Jawa Tengah

Kadwati, Kadwati; Hidayat, Sri Hendrastuti

doi:10.14692/jfi.11.4.121

Cited by 6 publications

(3 citation statements)

References 1 publication

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“…Gejala lebih lanjut dengan terbentuknya gejala garis kuning vertikal yang terlihat semakin jelas pada seluruh daun. Infeksi virus bahkan dapat menyebabkan tanaman kerdil dengan disertai mengecilnya ukuran daun [9].…”

Section: Pendahuluanunclassified

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Deteksi keberadaan virus pada sampel bawang merah (Allium ascalonicum) dan bawang putih (Allium sativum) di Laboratorium Balai Besar karantina Pertanian Makassar

Amrullah¹,

Zulkarnain²,

Juhari³

et al. 2023

Filogeni: J. Mahasiswa Bio.

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Tanaman dan bagian-bagiannya merupakan media pembawa organisme pengganggu tumbuhan (OPT) pada proses karantina tanaman dalam kegiatan pengiriman ekspor maupun impor ataupun pengiriman dari suatu wilayah ke wilayah lain di dalam negeri. OPT adalah semua organisme yang dapat menyebabkan dan menimbulkan kerusakan fisik, gangguan fisiologi dan biokimia, atau kompetisi hara terhadap tanaman budidaya. Salah satu jenis OPT pada tanaman yaitu virus dengan mekanisme penularan secara mekanis melalui organ vegetatif maupun melalui perantara vektor. Tujuan penelitian ini adalah untuk mendeteksi virus pada media pembawa (tanamana) yaitu bawang merah (Allium ascalonicum) dan bawang putih (A. sativum) yang merupakan komoditi pertanian penting. Metode yang digunakan yaitu uji serologi dengan metode DAS ELISA untuk mendeteksi keberadaan salah satu jenis virus dengan tingkat infeksi yang cukup tinggi pada tanaman bawang yaitu Onion yellow dwarf virus (OYDV). Hasil yang diperoleh dari kedua sampel bawang yaitu bahwa kedua jeni bawang, baik itu bawang merah maupun bawang putih negatif terinfeksi OYDV berdasarkan metode ELISA yang ditunjukkan dengan nilai absorbansi sampel tidak mencapai tiga kali lipat dari nilai absorbansi kontrol negatif serta tidak terjadi perubahan warna pada sumur sampel di microplate.

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Section: Pendahuluanunclassified

“…_ Filogeni: Jurnal Mahasiswa Biologi, Volume 3, Nomor 1, Januari-April 2023, hlm [6][7][8][9][10][11]. …”

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Deteksi keberadaan virus pada sampel bawang merah (Allium ascalonicum) dan bawang putih (Allium sativum) di Laboratorium Balai Besar karantina Pertanian Makassar

Amrullah¹,

Zulkarnain²,

Juhari³

et al. 2023

Filogeni: J. Mahasiswa Bio.

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“…Virus targets for this assay were OYDV, GCLV, and SLV. The colour intensity on the membrane was used to determine virus titre or concentration in the sample (Kadwati & Hidayat 2015) (Table 1). The average of virus titter was then calculated using MS Excel for each garlic variety.…”

Section: Assessment Of Virus Incidence and Titre By Dotmentioning

confidence: 99%

Incidence of main viruses infecting local garlic in Java, Indonesia

Hidayat

Meliyana

Yulianingsih

et al. 2023

J Trop Plant Pests Dis

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Virus infection is one of the challenges in garlic production due to it perpetuates from one generation to the next and its infection caused huge yield reduction. There was still few information regarding virus status on Indonesian local garlic cultivars. This study was aimed to detect four major viruses infecting local garlic in Indonesia, they were members of genus Potyvirus (Onion yellow dwarf virus/OYDV, Leek yellow stripe virus/LYSV), and Carlavirus (Garlic common laten virus/ GCLV and Shallot latent virus/SLV). Garlic samples were obtained from IPB University collection and field survey in Tegal and Karanganyar (Central Java Province). Dot immuno-binding assay (DIBA) was done for initial virus indexing on non-commercial and commercial cultivars. Reverse transcription polymerase chain reaction (RT-PCR) using four specific primers was done to detect virus on commercial cultivars. DIBA from leaf samples showed that virus incidence of OYDV was relatively higher (92.3 to 100%) than GCLV and SLV (84.6 to 100%) from all tested cultivars. On average, ‘Lumbu Hijau’ has the lowest level of virus titter (severity) than other cultivars. The virus incidence of both bulbil and single clover was similar (97 – 100%) while virus titter of OYDV, GCLV, and SLV on bulbil was the lowest than other propagation materials. Detection by RT-PCR from two commercial cultivars showed that ‘Lumbu Hijau’ has less virus incidence than ‘Jawa Lama’. LYSV, OYDV, GCLV were detected on both cultivars but SLV was not found. Further virus indexing using larger number of samples and involving more virus targets needs to be done.

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Variations of Garlic Common Latent Virus and Shallot Latent Virus Concentration on Shallot and Garlic

Pauzi

Lestari

Hidayat

2018

IOP Conf. Ser.: Earth Environ. Sci.

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Deteksi Virus Utama Bawang Merah dan Bawang Putih dari Daerah Jawa Barat dan Jawa Tengah

Cited by 6 publications

References 1 publication

Deteksi keberadaan virus pada sampel bawang merah (Allium ascalonicum) dan bawang putih (Allium sativum) di Laboratorium Balai Besar karantina Pertanian Makassar

Deteksi keberadaan virus pada sampel bawang merah (Allium ascalonicum) dan bawang putih (Allium sativum) di Laboratorium Balai Besar karantina Pertanian Makassar

Incidence of main viruses infecting local garlic in Java, Indonesia

Variations of Garlic Common Latent Virus and Shallot Latent Virus Concentration on Shallot and Garlic

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