Detection of Wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of Paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis

Parvizi, Parviz; Najafzadeh, Narmin

doi:10.1590/s0074-0276108042013004

Cited by 21 publications

(14 citation statements)

References 49 publications

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“…It indicated that one species of host can harbor more than one Wolbachia strain which are congruent with a previous study by Parvizi et al [24]. One species of Paraphlebotomus sandflies from Iran were believed to be infected by two Wolbachia strains, namely Turk 54 (wPap) and Turk 07 (novel strain) [24]. Another previous study congruent with our result is the study of Wolbachia infection in tephritid fruit flies from Thailand [23].…”

Section: Resultssupporting

confidence: 92%

“…Besides that, two strains of F. vandensboschi were infected and clustered into two different subgroups-Mel and Inc. It indicated that one species of host can harbor more than one Wolbachia strain which are congruent with a previous study by Parvizi et al [24]. One species of Paraphlebotomus sandflies from Iran were believed to be infected by two Wolbachia strains, namely Turk 54 (wPap) and Turk 07 (novel strain) [24].…”

Section: Resultssupporting

confidence: 84%

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Isolation and Characterization of Wolbachia (Rickettsiales: Rickettsiaceace) from Several Economic Importance Parasitoids (Hymenoptera: Braconidae)

Mohammed¹,

Zuki²,

Yusof³

et al. 2015

IJBBB

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Abstract:Endosymbiotic relationship between Wolbachia (Rickettsiales: Rickettsiaceace) and braconids species is a new topic to be discussed among researchers due to its potential as biocontrol agents of fruit flies species. In this study, Wolbachia Surface Protien (wsp) marker has been used to detect the existence of Wolbachia molecularly that associated with braconids (Opiinae) collected from several localities throughout Peninsular Malaysia. Our results revealed that five species of parasitoids viz. Fopius arisanus, F. vandensboschi, Psyttalia sp., P. fletcheri and P. incisi were symbiont positively with nine different strains of Wolbachia. A total of nine strains have been successfully sequenced and classified into three Wolbachia subgroups namely Mel, Ri, and Inc based on the results of clustering analysis. In this study, Inc Wolbachia group has been known as a novel group emerged of Wolbachia. These data should be useful in future Wolbachia-based biological control program that involving parasitoids and fruit flies.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 84%

Isolation and Characterization of Wolbachia (Rickettsiales: Rickettsiaceace) from Several Economic Importance Parasitoids (Hymenoptera: Braconidae)

Mohammed¹,

Zuki²,

Yusof³

et al. 2015

IJBBB

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“…This study documented that two braconid species, F. arisanus and F. vandenboschi, were infected by multiple strains of Wolbachia. Similar infections by more than one Wolbachia strains have been reported in other insects as well: one species of Paraphlebotomus sandfly from Iran was believed to be infected by two Wolbachia strains (Parvizi et al 2013); and Bactrocera ascita sp.B from Thailand was infected by five Wolbachia strains (Jamnongluk et al 2002). More extensive and intensive surveys for Wolbachia will find more such instances that one species is infected by multiple strains.…”

Section: Wolbachia Infection Status Of Braconid and Tephritid Populatsupporting

confidence: 68%

“…Similar infections by more than one Wolbachia strains have been reported in other insects as well: one species of Paraphlebotomus sandfly from Iran was believed to be infected by two Wolbachia strains (Parvizi et al . ); and Bactrocera ascita sp.B from Thailand was infected by five Wolbachia strains (Jamnongluk et al . ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and evolutionary history of endosymbiont Wolbachia (Rickettsiales: Anaplasmataceae) in parasitoids (Hymenoptera: Braconidae) associated with Bactrocera fruit flies (Diptera: Tephritidae) infesting carambola

Mohammed

Aman-Zuki

Yusof

et al. 2017

Entomological Science

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Wolbachia endosymbiont is a maternally inherited bacteria that infects a wide range of hosts, including parasitoids and their respective hosts. In this study, a total of 171 individuals of braconid endoparasitoids, consisting of Fopius arisanus, F. vandenboschi, Diachasmimorpha longicaudata, Psyttalia sp.1, Psyttalia sp.2, P. fletcheri and P. incisi, and their host tephritid fruit flies of Bactrocera dorsalis, B. papayae and B. carambolae infesting carambola were screened molecularly by the Wolbachia surface protein (wsp) gene. Interestingly, 21 (24.14%) wsp gene sequences were successfully isolated from 87 braconid samples tested, showing a low infection rate of Wolbachia. However, despite the close ecological contact between parasitoids and their hosts, none of the tephritid individuals were infected by Wolbachia. A comparison of wsp and host mitochondrial cytochrome c oxidase subunit I (COI) sequences found that braconids did not cluster in connection with Wolbachia infection, suggesting that selective sweep has not yet occurred because Wolbachia may have recently infected the braconid populations in Peninsular Malaysia (≈0.1 MYA). Despite of relatively recent infections of Wolbachia, the history of Wolbachia infection into F. arisanus populations of Peninsular Malaysia is complex, involving at least two independent occasions of infection and two secondary losses.

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“…Many investigations have been conducted on different aspects of ZCL in naturally important foci in Iran [9][10][11] , although some areas of neighboring Provinces have been neglected for unknown reasons . Predisposing factors, such as increasing migration of patients from endemic foci to potential areas, irregular construction and urbanization and changing sandfl y fauna in the region affect the distribution and survival of ZCL 9,12 .…”

Section: Introductionmentioning

confidence: 99%

The existence of only one haplotype of Leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in Iran

Najafzadeh

Sedaghat

Sultan

et al. 2014

Rev. Soc. Bras. Med. Trop.

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Introduction: Leishmania major is the causative agent of zoonotic cutaneous leishmaniasis (ZCL), and great gerbils are the main reservoir hosts in Iran. Abarkouh in central Iran is an emerging focal point for which the reservoir hosts of ZCL are unclear. This research project was designed to detect any Leishmania parasites in different wild rodent species. Methods: All rodents captured in 2011 and 2012 from Abarkouh district were identifi ed based on morphological characteristics and by amplifi cation of the rodent cytochrome b (Cyt b) gene. To detect Leishmania infection in rodents, deoxyribonucleic acid (DNA) of each ear was extracted. Internal transcribed spacer-ribosomal deoxyribonucleic acid (ITS-rDNA), microsatellites, kinetoplast deoxyribonucleic acid (kDNA) and cytochrome b genes of Leishmania parasites were amplifi ed by polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) and sequencing were employed to confi rm the Leishmania identifi cation. Results: Of 68 captured rodents in the region, 55 Rhombomys opimus were identifi ed and nine Leishmania infections (9/55) were found. In addition, eight Meriones libycus and two Tatera indica were sampled, and one of each was confi rmed to be infected. Two Meriones persicus and one Mus musculus were sampled with no infection. Conclusions: The results showed that all 11 unambiguously positive Leishmania infections were Leishmania major. Only one haplotype of L. major (GenBank access No. EF413075) was found and at least three rodents R. opimus, M. libycus and T. indica-appear to be the main and potential reservoir hosts in this ZCL focus. The reservoir hosts are variable and versatile in small ZCL focal locations.

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Detection of Wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of Paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis

Cited by 21 publications

References 49 publications

Isolation and Characterization of Wolbachia (Rickettsiales: Rickettsiaceace) from Several Economic Importance Parasitoids (Hymenoptera: Braconidae)

Isolation and Characterization of Wolbachia (Rickettsiales: Rickettsiaceace) from Several Economic Importance Parasitoids (Hymenoptera: Braconidae)

Prevalence and evolutionary history of endosymbiont Wolbachia (Rickettsiales: Anaplasmataceae) in parasitoids (Hymenoptera: Braconidae) associated with Bactrocera fruit flies (Diptera: Tephritidae) infesting carambola

The existence of only one haplotype of Leishmania major in the main and potential reservoir hosts of zoonotic cutaneous leishmaniasis using different molecular markers in a focal area in Iran

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