Detection of vascular endothelial growth factor based on rolling circle amplification as a means of signal enhancement in surface plasmon resonance

Electrochemically triggered aptamer immobilization via click reaction for vascular endothelial growth factor detection

“…The different proteins act as control to exclude a possible target binding based on bare electrostatic interaction; however, the current signal change was mainly ascribed to aptamer structure change after specific target binding. The detection performances are comparable with previous reported methods, as summarized in Table 1 [11][12][13][14][15][33][34][35][36][37][38]. Different from the previous work using complicated device fabrication processes or antibodies/RNA aptamers [11][12][13], our present method relies on a simple electrochemical click method using more economic and stable DNA aptamer as recognition motif.…”

G-quadruplex DNAzyme-based electrochemiluminescence biosensing strategy for VEGF165 detection: Combination of aptamer–target recognition and T7 exonuclease-assisted cycling signal amplification

“…As shown in the inset in Fig. 4, a broad linear relationship between the differences in the ECL intensity and the logarithm of concentration of VEGF 165 in the range of 1 pM to 20 nM with a correlation coefficient (R) of 0.996, and the linear fitting equation is ΔI¼882.14-1432.2 lg [c], where ΔI is I 0 À I, c is the concentration of VEGF 165 ; this range was wider than those in previous reports (Zhao et al, 2012;Freeman et al, 2012;Kopra et al, 2014;Mita et al, 2014;Cho et al, 2012;Chen et al, 2014). Detection limit was experimentally estimated to be 0.2 pM, which was lower than that in fluorescence (Freeman et al, 2012;Kopra et al, 2014) and electrochemical method (Nonaka et al, 2012) (the detailed comparison of the analytical performance for VEGF 165 detection by using our strategy and those reported in the literature is summarized in Table 1).…”

A high sensitive electrochemical aptasensor for the determination of VEGF165 in serum of lung cancer patient

“…Up to now, various analytical methods have been reported in the literature for the determination of VEGF 165 , such as optical methods (Freeman et al 2012;Gohring et al 2010;Kopra et al 2014; M.A.A-Ameen and G.Ghosh 2013; Wang et al 2014a;Wang et al 2014;Al-Ameen and Ghosh 2013;Cho et al 2012;Kopra et al 2014;Urmann et al 2015), piezoelectric micro cantilever (Capobianco et al 2011), field-effect transistor (FET) Kwona et al 2010; O.S. Kwon et al 2012;Chen et al 2014a) surface plasmon resonance (SPR) Uludag and Tothill 2012;Y.Li et al 2007;Chen et al 2014b), quartz crystal microbalance (QCM) (Cabric et al 2010;Uludag and Tothill 2012), surface-enhanced Raman scattering (SERS) (Ko et al 2013;Zhao et al 2015), enzyme-linked immunosorbent assay (ELISA) (Hsu et al 2015;Yip-Schneider et al) and electrochemical aptasensors (Li et al 2007;Lin et al 2015;Lv et al 2013;May et al 2005;Sassolas et al 2009;Wang et al 2014b;Zhao et al 2011). …”