Detection of uric acid at reversibly nanostructured thin-film microelectrodes

Herrasti, Z.; Martínez, Fernando Rey; Baldrich, Eva

doi:10.1016/j.snb.2016.05.018

Cited by 25 publications

(16 citation statements)

References 27 publications

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“…To date, various techniques have been used for the quantitative analysis of UA, such as high-performance liquid chromatography (HPLC) [ 11 ], fluorescence spectroscopy [ 12 ], electrochemistry [ 13 ], ultraviolet (UV) absorption spectroscopy [ 14 ], colorimetric assays [ 15 ], chemiluminescence [ 16 ], and surface-enhanced Raman spectroscopy [ 17 ]. However, these techniques only allow for the detection of a single signal.…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

Rui

Yan

et al. 2021

Microchim Acta

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Graphical abstract A simple dual-read assay for uric acid (UA) was developed based on a combined ratiometric fluorescent and colorimetric strategy using nitrogen-doped carbon dots (N-CDs). The biosensor relies on the oxidation of UA by uricase to produce H 2 O 2 , which was then converted to • OH radicals by I - , resulting in the oxidation of o -phenylenediamine (OPD) to 2,3-diaminophenazine (DAP). In the presence of UA, the colorless biosensor system changed to yellow. Furthermore, the presence of DAP quenched the fluorescence emission of the N-CDs at 427 nm based on the inner filter effect (IFE). With increasing UA concentrations, the fluorescence intensity of the biosensor at 427 nm decreased but increased at 580 nm, demonstrating the ratiometric response. A strong linearity was observed between the fluorescence intensity ratio of DAP to N-CDs ( I 580 / I 427 ) and the corresponding UA concentration over the range 0.5−150 μM, and a limit of detection (S/N ratio of 3) of 0.06 μM was calculated. The dual-read assay was successfully employed in the quantitation of UA in human serum and urine samples, revealing its potential for measuring UA in clinical samples. Supplementary Information The online version contains supplementary material available at 10.1007/s00604-021-04971-2.

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Section: Introductionmentioning

confidence: 99%

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

Rui

Yan

et al. 2021

Microchim Acta

View full text Add to dashboard Cite

show abstract

“…As the most optimal research object, urine can be noninvasively collected and carries important physical and chemical information related to many metabolic diseases, such as diabetes, gouty arthritis, and renal disease diacrisis. [ 16 , 17 ] Many methods have been established to measure the UA level in urine, including absorption spectrophotometry, [ 18 ] fluorescence biosensors, [ 17 , 19 ] electrochemical biosensors, [ 20 , 21 ] high‐performance liquid chromatography‐ultraviolet (HPLC‐UV), [ 22 ] and HPLC‐mass spectrometry, [ 23 ] all of which can be employed to monitor UA in urine with the merits of high sensitivity and excellent specificity to facilitate the diagnosis of hyperuricemia. Noticeably, the use of harmful reagents as mobile phase or solvent poses a threat to the environment and human health, which deviates from the concept of green development.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive and Individual‐Centered Monitoring of Uric Acid for Precaution of Hyperuricemia via Optical Supramolecular Sensing

Zhang

Chai

et al. 2022

Advanced Science

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Characterized by an excessively increased uric acid (UA) level in serum, hyperuricemia induces gout and also poses a great threat to renal and cardiovascular systems. It is urgent and meaningful to perform early warning by noninvasive diagnosis, thus conducing to blockage of disease aggravation. Here, guanidinocalix[5]arene (GC5A) is successfully identified from the self-built macrocyclic library to specifically monitor UA from urine by the indicator displacement assay. UA is strongly bound to GC5A at micromolar-level, while simultaneously excluding fluorescein (Fl) from the GC5A•Fl complex in the "switch-on" mode. This method successfully differentiates patients with hyperuricemia from volunteers except for those with kidney dysfunction and targets a volunteer at high risk of hyperuricemia. In order to meet the trend from hospital-centered to individual-centered testing, visual detection of UA is studied through a smartphone equipped with a color-scanning feature, whose adaptability and feasibility are demonstrated in sensing UA from authentic urine, leading to a promising method in family-centered healthcare style. A high-throughput and visual detection method is provided here for alarming hyperuricemic by noninvasive diagnosis.

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“…Since purine is disintegrated into uric acid, the UA concentration is related to purine metabolism and normally remains in a stable range for healthy persons. The normal concentration of uric acid present in the blood is in the range of 3-7 mg/dL, whereas that excreted in the urine is about 16-100 mg/dL per 24 h. [1][2][3] A high purine intake from diet produces an excessive amount of uric acid in the human body. When the excessive uric acid gets concentrated and crystallized, the crystallites cause numerous illnesses.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of uric acid using paper-based biosensor modified with graphene oxide and 5-amino-1,3,4-thiadiazole-2-thiol

2022

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Detection of uric acid at reversibly nanostructured thin-film microelectrodes

Cited by 25 publications

References 27 publications

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

Noninvasive and Individual‐Centered Monitoring of Uric Acid for Precaution of Hyperuricemia via Optical Supramolecular Sensing

Quantitative determination of uric acid using paper-based biosensor modified with graphene oxide and 5-amino-1,3,4-thiadiazole-2-thiol

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