Detection of terminal deletions in barley chromosomes by the PCR based method.

Yoshino, Mitsuaki; Nasuda, Shuhei; Endo, Takashi R.

doi:10.1266/ggs.73.163

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…The FISH probe was one of the barley subtelomeric sequences (HvT01) which is repeated in tandem arrays and is closely associated with the telomeric repeats (Belostotsky and Ananiev, 1990;Roder et al, 1993). This probe was labeled with Dig-dUTP (Boehringer) via PCR amplification as described by Schubert et al (1998), using the total barley genomic DNA or HvT01 clone as template and a primer set designed by Yoshino et al (1998). The total barley genomic DNA was used for the GISH probe.…”

Section: Cytology Structural Changes In Barley Chromosomesmentioning

confidence: 99%

Genetic induction of structural changes in barley chromosomes added to common wheat by a gametocidal chromosome derived from Aegilop Scylindrica.

Shi

Endo

1999

Genes Genet. Syst.

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Section: Cytology Structural Changes In Barley Chromosomesmentioning

confidence: 99%

Genetic induction of structural changes in barley chromosomes added to common wheat by a gametocidal chromosome derived from Aegilop Scylindrica.

Shi

Endo

1999

Genes Genet. Syst.

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“…As shown in Table 2, Goff et al 2002;Yu et al 2002). Should segments smaller than chromosome arms be needed, it is possible to generate these via the activity of gametocidal chromosomes (Shi & Endo 1997, Yoshino et al 1998). Thus, it may be possible in the future to isolate, via flow sorting, portions of the Triticeae genomes smaller than a chromosome arm.…”

Section: A Flow Cytogenetic Toolbox For the Triticeaementioning

confidence: 99%

Chromosome-based genomics in the cereals

et al. 2007

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The cereals are of enormous importance to mankind. Many of the major cereal species Y specifically, wheat, barley, oat, rye, and maize Y have large genomes. Early cytogenetics, genome analysis and genetic mapping in the cereals benefited greatly from their large chromosomes, and the allopolyploidy of wheat and oats that has allowed for the development of many precise cytogenetic stocks. In the genomics era, however, large genomes are disadvantageous. Sequencing large and complex genomes is expensive, and the assembly of genome sequence is hampered by a significant content of repetitive DNA and, in allopolyploids, by the presence of homoeologous genomes. Dissection of the genome into its component chromosomes and chromosome arms provides an elegant solution to these problems. In this review we illustrate how this can be achieved by flow cytometric sorting. We describe the development of methods for the preparation of intact chromosome suspensions from the major cereals, and their analysis and sorting using flow cytometry. We explain how difficulties in the discrimination of specific chromosomes and their arms can be overcome by exploiting extant cytogenetic stocks of polyploid wheat and oats, in particular chromosome deletion and alien addition lines. Finally, we discuss some of the applications of flow-sorted chromosomes, and present some examples demonstrating that a chromosome-based approach is advantageous for the analysis of the complex genomes of cereals, and that it can offer significant potential for the delivery of genome sequencing and gene cloning in these crops.

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Dissection of the nuclear genome of barley by chromosome flow sorting

et al. 2006

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Isolation of mitotic chromosomes using flow cytometry is an attractive way to dissect nuclear genomes into their individual chromosomal components or portions of them. This approach is especially useful in plants with complex genomes, where it offers a targeted and hence economical approach to genome analysis and gene cloning. In several plant species, DNA of flow-sorted chromosomes has been used for isolation of molecular markers from specific genome regions, for physical mapping using polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH), for integration of genetic and physical maps and for construction of chromosome-specific DNA libraries, including those cloned in bacterial artificial chromosome vectors. Until now, chromosome analysis and sorting using flow cytometry (flow cytogenetics) has found little application in barley (2n = 14, 1C approximately 5,100 Mbp) because of the impossibility of discriminating and sorting individual chromosomes, except for the smallest chromosome 1H and some translocation chromosomes with DNA content significantly different from the remaining chromosomes. In this work, we demonstrate that wheat-barley ditelosomic addition lines can be used to sort any arm of barley chromosomes 2H-7H. Thus, the barley genome can be dissected into fractions representing only about 6-12% of the total genome. This advance makes the flow cytogenetics an attractive tool, which may greatly facilitate genome analysis and gene cloning in barley.

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Detection of terminal deletions in barley chromosomes by the PCR based method.

Cited by 4 publications

References 15 publications

Genetic induction of structural changes in barley chromosomes added to common wheat by a gametocidal chromosome derived from Aegilop Scylindrica.

Genetic induction of structural changes in barley chromosomes added to common wheat by a gametocidal chromosome derived from Aegilop Scylindrica.

Chromosome-based genomics in the cereals

Dissection of the nuclear genome of barley by chromosome flow sorting

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