Detection of T lymphocyte subsets and related functional molecules in follicular fluid of patients with polycystic ovary syndrome

Abstract

Immune responses play an important role in the pathogenesis of polycystic ovary syndrome (PCOS). However, the characteristics of T lymphocyte subsets in PCOS remain insufficiently understood. In this study, lymphocytes of follicular fluid (FF) were obtained from oocyte retrieval before in-vitro fertilization (IVF) in infertile women with or without PCOS. The levels of cluster of differentiation 25 (CD25), CD69, programmed death 1 (PD-1), interferon-γ (IFN-γ), interleukin 17A (IL-17A) and IL-10 in T lymphocytes were determined by flow cytometry. Our results showed that the percentage of FF CD8 + T cells was significantly decreased in infertile patients with PCOS ( P < 0.05). Furthermore, the levels of CD69 and IFN-γ were significantly decreased and the level of PD-1 was increased in both CD4 + and CD8 + T cells from infertile patients with PCOS ( P < 0.05). Moreover, the expression of PD-1 on CD4 + or CD8 + T cells was positively correlated with the estradiol (E2) levels in the serum and reversely correlated with the expression of IFN-γ in CD4 + or CD8 + T cells in infertile patients with PCOS. These results suggested that T cell dysfunction may be involved in the pathogenesis of PCOS.

<p>Peripheral Blood Inflammatory-Immune Cells as a Predictor of Infertility in Women with Polycystic Ovary Syndrome</p>

“…Li et al also show that there is no difference in T between PCOS and controls. 17 Consistent with previous reports, women with PCOS have higher WBC, Neu, and Lym counts than the control group in our study. PCOS shares a state of low-grade inflammation with other non-transmissible chronic diseases, such as obesity, T2DM, and cardiovascular diseases.…”

“…Previous studies showed that abnormal activation of T cells and cytokine production might lead to the abnormal oocyte development observed in PCOS patients, which was associated with women's infertility. 17,28 A recent study also found that the activation of T cells in PCOS patients with infertility might be suppressed by increased expression of PD-1. IL-10 markedly inhibits the functions of monocytesmacrophages, such as antigen presentation.…”

“…IL-10 markedly inhibits the functions of monocytesmacrophages, such as antigen presentation. 17 Therefore, the lymphocyte subsets were evaluated and T cell subsets were focused in our research. Unexpectedly, T cell subsets and B cells did not seem to differ from infertile women to pregnant women with PCOS (P >0.05).…”

Impaired CD8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells Promote The Development of Polycystic Ovary Syndrome

“…Recently, researches have focused on the association between immune disorders and PCOS, such as dysfunction of macrophages, dendritic cells and T regulatory cells (Tregs) [6][7][8][9][10][11].…”

Acorus gramineusand and Euodia ruticarpa Steam Distilled Essential Oils Exert Anti-Inflammatory Effects Through Decreasing Th1/Th2 and Pro-/Anti-Inflammatory Cytokine Secretion Ratios In Vitro

“…Th1 polarization may induce autoimmune diseases including multiple sclerosis, inflammatory bowel disease, rheumatoid arthritis, etc., but Th2-inclination may result in allergic diseases and susceptibility to infection [10,13]. T lymphocyte subsets can be detected by changes in cytokine expression, unique surface markers and nuclear transcription factors [13,14]. The Th1/Th2 balance was found influenced by nutrients, hormones, omega-3 fatty acids, plant sterols/sterolins, particular minerals, probiotics, progesterone and melatonin, suggesting that Th1/Th2-based immunotherapies are promising to date [10].…”