Detection of Single Mammalian Cells by High-Resolution Magnetic Resonance Imaging

Dodd, Stephen J.; Williams, M. A.; Suhan, Joseph; Williams, Donald S.; Koretsky, Alan P.; Ho, Chien

doi:10.1016/s0006-3495(99)77182-1

Cited by 261 publications

(205 citation statements)

References 14 publications

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“…68,80 The potential of in vivo cell tracking has been demonstrated in several systems including SPIO loaded T cells. 31 Objects as diminutive as single cells were able to be monitored since the T2 relaxation effect of the sequestered magnetic nanoparticles is exerted over a region larger than that of the cell itself. SPIO labeled T cells showed a 20% reduction of signal in the spleen of mice at 3 h postinjection.…”

Section: Cell Trackingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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Abstract-The field of molecular imaging has recently seen rapid advances in the development of novel contrast agents and the implementation of insightful approaches to monitor biological processes non-invasively. In particular, superparamagnetic iron oxide nanoparticles (SPIO) have demonstrated their utility as an important tool for enhancing magnetic resonance contrast, allowing researchers to monitor not only anatomical changes, but physiological and molecular changes as well. Applications have ranged from detecting inflammatory diseases via the accumulation of non-targeted SPIO in infiltrating macrophages to the specific identification of cell surface markers expressed on tumors. In this article, we attempt to illustrate the broad utility of SPIO in molecular imaging, including some of the recent developments, such as the transformation of SPIO into an activatable probe termed the magnetic relaxation switch.

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Section: Cell Trackingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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“…Super paramagnetic iron oxide (whole particle diameter B30 nm (Dodd et al, 1999), obtained from Chien Ho's laboratory at Carnegie Mellon University) was prepared for intravenous injection at a concentration of 15 mg Fe/kg body weight. CBV tweighted fMRI studies were performed by acquisition of gradient-echo (GE) echo planar images with TR = 1 s, and TE = 20 and 10 msecs before and after MION injection, respectively.…”

Section: Magnetic Resonance Acquisitionsmentioning

confidence: 99%

Arterial versus Total Blood Volume Changes during Neural Activity-Induced Cerebral Blood Flow Change: Implication for BOLD fMRI

Kim

Hendrich

Masamoto

et al. 2006

J Cereb Blood Flow Metab

170

198

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Quantifying both arterial cerebral blood volume (CBV a ) changes and total cerebral blood volume (CBV t ) changes during neural activation can provide critical information about vascular control mechanisms, and help to identify the origins of neurovascular responses in conventional blood oxygenation level dependent (BOLD) magnetic resonance imaging (MRI). Cerebral blood flow (CBF), CBV a , and CBV t were quantified by MRI at 9.4 T in isoflurane-anesthetized rats during 15-s duration forepaw stimulation. Cerebral blood flow and CBV a were simultaneously determined by modulation of tissue and vessel signals using arterial spin labeling, while CBV t was measured with a susceptibility-based contrast agent. Baseline versus stimulation values in a region centered over the somatosensory cortex were: CBF = 150618 versus 182620 mL/100 g/min, CBV a = 0.8360.21 versus 1.1760.30 mL/100 g, CBV t = 3.1060.55 versus 3.4160.61 mL/100 g, and CBV a /CBV t = 0.2760.05 versus 0.3460.06 (n = 7, mean6s.d.). Neural activity-induced absolute changes in CBV a and CBV t are statistically equivalent and independent of the spatial extent of regional analysis. Under our conditions, increased CBV t during neural activation originates mainly from arterial rather than venous blood volume changes, and therefore a critical implication is that venous blood volume changes may be negligible in BOLD fMRI.

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“…Measured on the basis of the absolute concentration of particles in tissue, SPIO in T2 and T2*-weighted images result in a greater negative contrast (detectability mmol-μmol) [29] than comparable concentrations of paramagnetic gadolinium (Gd)-containing contrast agent (detectability to cmol-mmol), making SPIO particularly preferred for use in molecular imaging. With respect to the selection of MR sequences and sequence parameters, it must be stated that gradient echo sequences (GRE, FFE) are more sensitive with respect to magnetic susceptibilities than spin echo sequences (SE) and the sensitivity of the sequence increases with the decrease of the flip angle (FA< 20°), extension of the echo time (TE> 10 -20 ms) and repetition time (TR> 100 ms), and the increase of the spatial resolution (reduction of the partial volume effect) [30]. In addition to these negative-contrast techniques, newer developments in susceptibility-weighted imaging (SWI) use magnitude information as well as phase information from the complex data of spatially highly resolved 3 D gradient echo sequences to generate tissue imaging with improved contrast without using a contrast agent [31 -33] or a positivecontrast image after the administration of a contrast agent (e. g. SPIO) [34,35].…”

mentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Ittrich¹,

Peldschus²,

Raabe³

et al. 2013

Fortschr Röntgenstr

124

101

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Superparamagnetic iron oxide nanoparticles (SPIO) can be used to image physiological processes and anatomical, cellular and molecular changes in diseases. The clinical applications range from the imaging of tumors and metastases in the liver, spleen and bone marrow, the imaging of lymph nodes and the CNS, MRA and perfusion imaging to atherosclerotic plaque and thrombosis imaging. New experimental approaches in molecular imaging describe undirected SPIO trapping (passive targeting) in inflammation, tumors and associated macrophages as well as the directed accumulation of SPIO ligands (active targeting) in tumor endothelia and tumor cells, areas of apoptosis, infarction, inflammation and degeneration in cardiovascular and neurological diseases, in atherosclerotic plaques or thrombi. The labeling of stem or immune cells allows the visualization of cell therapies or transplant rejections. The coupling of SPIO to ligands, radio- and/or chemotherapeutics, embedding in carrier systems or activatable smart sensor probes and their externally controlled focusing (physical targeting) enable molecular tumor therapies or the imaging of metabolic and enzymatic processes. Monodisperse SPIO with defined physicochemical and pharmacodynamic properties may improve SPIO-based MRI in the future and as targeted probes in diagnostic magnetic resonance (DMR) using chip-based ?NMR may significantly expand the spectrum of in vitro analysis methods for biomarker, pathogens and tumor cells. Magnetic particle imaging (MPI) as a new imaging modality offers new applications for SPIO in cardiovascular, oncological, cellular and molecular diagnostics and therapy. Key Points: Citation Format:

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Detection of Single Mammalian Cells by High-Resolution Magnetic Resonance Imaging

Cited by 261 publications

References 14 publications

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Arterial versus Total Blood Volume Changes during Neural Activity-Induced Cerebral Blood Flow Change: Implication for BOLD fMRI

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

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