2021
DOI: 10.1016/j.scitotenv.2020.142645
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Abstract: The world is experiencing the worst global health crisis in recent decades since December/2019 due to a new pandemic coronavirus. The COVID-19 disease, caused by SARS-CoV-2, has resulted in more than 30 million cases and 950 thousand deaths worldwide as of September 21, 2020. Determining the extent of the virus on public surfaces is critical for understanding the potential risk of infection in these areas. In this study, we investigated the presence of SARS-CoV-2 RNA on public surfaces in a densely populated u… Show more

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Cited by 51 publications
(66 citation statements)
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“…The data used to quantify risks from measured concentrations of SARS-CoV-2 RNA on surfaces in public spaces were obtained from two locations: Somerville, Massachusetts, USA, 15 and Belo Horizonte, Minas Gerais State, Brazil. 13 The sampling collection for both studies occurred throughout a COVID-19 outbreak from March–June 2020. Both places had control measures when the collection took place, including mandatory use of masks in public spaces.…”
Section: Resultsmentioning
confidence: 99%
“…The data used to quantify risks from measured concentrations of SARS-CoV-2 RNA on surfaces in public spaces were obtained from two locations: Somerville, Massachusetts, USA, 15 and Belo Horizonte, Minas Gerais State, Brazil. 13 The sampling collection for both studies occurred throughout a COVID-19 outbreak from March–June 2020. Both places had control measures when the collection took place, including mandatory use of masks in public spaces.…”
Section: Resultsmentioning
confidence: 99%
“…These are perhaps the factors that have led to the contrasting results from the 2003 finding at the Hong Kong Metropole hotel elevator area, where SARS-CoV viral RNA was detected even three months after the case had left the hotel [ 28 ]. In addition, SARS-CoV-2 viral RNA was detected on public benches in Brazil [ 29 ], but detection of SARS-CoV-2 in that instance could also be explained by its location in a city with a high number of notified COVID-19 cases, and that regular disinfection had not been carried out.…”
Section: Resultsmentioning
confidence: 99%
“…Nucleic acid extraction and the RT-qPCR analysis were performed at the Virus Lab, similarly to recent work for detection of SARS-CoV-2 RNA on surfaces carried out by this same group ( Abrahão et al, 2020 ). Briefly, for each sample after particle extraction, 70 μL of transport solution was submitted to nucleic acid extraction using the QIAmp Viral RNA Mini Kit (QIAGEN®).…”
Section: Methodsmentioning
confidence: 99%
“…To date, for PCR quantification (based on standard curve) we considered 1 SARS-CoV-2 plaque forming unit as 1 SARS-CoV-2 genomic unit. Quantification was based only on the N1 target gene given the high efficiency of our standard curve ( Abrahao et al, 2020 ). N2 target gene quantification was not performed due to a low efficiency achieved for the standard curve for this target, as described elsewhere ( Abrahao et al, 2020 ).…”
Section: Methodsmentioning
confidence: 99%