Detection of Rotavirus Types G8 and G10 among Brazilian Children with Diarrhea

Santos, Norma; Lima, Rita C. C.; Pereira, Cláudio F. A.; Gouvêa, Vera

doi:10.1128/jcm.36.9.2727-2729.1998

Cited by 133 publications

(65 citation statements)

References 15 publications

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“…Human GAR G8 strains have been associated with long, short, and super-short electropherotype patterns [Hasegawa et al, 1984;Gerna et al, 1990;Adah et al, 1997;Rao et al, 2003]. Moreover, G8 strains have been also detected in human GAR samples exhibiting both long and short electropherotypes, highlighting the finding of more unusual combinations due to the close proximity of humans and animals [Santos et al, 1998;O'Halloran et al, 2000]. Interestingly, GAR strain B12 exhibited a long RNA pattern ( Supplementary Fig.…”

Section: Resultsmentioning

confidence: 96%

Full genomic analysis of a G8P[1] rotavirus strain isolated from an asymptomatic infant in Kenya provides evidence for an artiodactyl‐to‐human interspecies transmission event

Ghosh

Gatheru

Nyangao

et al. 2010

Journal of Medical Virology

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Group A rotavirus (GAR) G8P[1] strains, found sometimes in cattle, have been reported rarely from humans. Therefore, analysis of the full genomes of human G8P[1] strains are of significance in the context of studies on interspecies transmission of rotaviruses. However, to date, only partial-length nucleotide sequences are available for the 11 genes of a single human G8P[1] strain, while the partial sequences of two other strains have been reported. The present study reports the first complete genome sequence of a human G8P[1] strain, B12, detected from an asymptomatic infant in Kenya in 1987. By nucleotide sequence identities and phylogenetic analyses, the full-length nucleotide sequences of VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes of strain B12 were assigned to G8-P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3 genotypes, respectively. Each of the 11 genes of strain B12 appeared to be more related to cognate genes of artiodactyl (ruminant and/or camelid) and/or artiodactyl-derived human GAR strains than those of most other rotaviruses. Strain B12 exhibited low levels of genetic relatedness to canonical human GAR strains, such as Wa and DS-1, ruling out the possibility of its origin from reassortment events between artiodactyl-like human and true human strains. These observations suggest that strain B12 might have been directly transmitted from artiodactyls to humans. Unhygienic conditions and close proximity of humans to livestock at the sampling site might have facilitated this rare event. This is the first report on a full genomic analysis of a rotavirus strain from Kenya. To our knowledge, strain B12 might be the oldest G8 strain characterized molecularly from the Africa continent.

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Section: Resultsmentioning

confidence: 96%

Full genomic analysis of a G8P[1] rotavirus strain isolated from an asymptomatic infant in Kenya provides evidence for an artiodactyl‐to‐human interspecies transmission event

Ghosh

Gatheru

Nyangao

et al. 2010

Journal of Medical Virology

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“…Based on accumulated evidence, it is clear that GARs can transfer between pigs and humans, making pigs a potential reservoir for the emergence of unusual or novel strains of human GARs [13][14][15][16][17][18]. In fact, the novel G26 strain we found was most similar to the human 57vp7w strain, which can also be considered to be a G26 strain.…”

Section: Discussionmentioning

confidence: 92%

“…The zoonotic potential of animal GARs is a great concern [12]. GARs with unusual G genotypes commonly found in pigs and cattle have been detected in sporadic and epidemic cases of diarrhea in human populations [13][14][15][16][17][18]. A full genome-based genotyping system proposed by Matthijnssens et al revealed the existence of reassortant strains and the close relationship between human and animal strains [2,3].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of group A rotaviruses associated with repeated outbreaks of diarrhea in a farrow-to-finish farm: identification of a porcine rotavirus strain bearing a novel VP7 genotype, G26

Miyazaki

Kuga

Suzuki

et al. 2011

Vet Res

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Group A rotaviruses (GARs) are one of the most common causes of diarrhea in suckling pigs. Although a number of G and P genotypes have been identified in porcine GARs, few attempts have been made to study the molecular epidemiology of these viruses associated with diarrhea outbreaks within a farm over an extended period of time. Here, we investigated the molecular characteristics of GARs that caused four outbreaks of diarrhea among suckling pigs in a farrow-to-finish farm over the course of a year. G and P genotyping of GARs detected at each outbreak demonstrated genetic diversity in this farm as follows: G9P[23] was detected at the first outbreak, G9P[13]/[22] and G9P[23] at the second, G3P[7] at the third, and G9P[23], G5P[13]/[22], and P[7] combined with an untypeable G genotype at the fourth. Sequence analysis of the detected GARs revealed that such genetic diversity could have resulted not only from the introduction of new GAR strains, but also from gene reassortment between GAR strains within the farm. Further, the GAR strain carrying the untypeable G genotype was shown to be a novel porcine GAR bearing a new G26 genotype, as confirmed by the Rotavirus Classification Working Group.

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“…Eighteen studies reported that none of the rotavirus specimens possessed an unusual combination of serotype and electropherotype. However, several studies reported strains having an unusual combination of serotype and electropherotype (4,6,16,55,57,64,72,74). The frequency of the isolation of these rotaviruses varied from nil (0/605 in New England) (7) to 2.7% (9/335 in Bangladesh) (72).…”

Section: Discussionmentioning

confidence: 99%

“…In Malawi, G8 (51.0%) predominated in the 97-98 season (16). Serotype G5 (24.4%) and G10 (16.3%)'rotaviruses were second and third most frequently detected in Brazil during the period between 1996 and 1997 (57). In the United States in the 96-97 season, G9 was found in 7.2% of the rotaviruses (55).…”

Section: Discussionmentioning

confidence: 99%

The Relative Frequencies of G Serotypes of Rotaviruses Recovered from Hospitalized Children with Diarrhea: A 10‐Year Survey (1987–1996) in Japan with a Review of Globally Collected Data

Koshimura

Nakagomi

2000

Microbiology and Immunology

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Since rotavirus vaccines aim to protect children from severe diarrhea, knowledge of the prevailing G serotypes among rotaviruses from hospitalized children is essential. Thus, we determined the G serotypes of rotaviruses collected from children with acute diarrhea in a local referral hospital in Akita, Japan, over the 10-year period between January 1987 and December 1996. Based on the assumption that rotaviruses with an identical electropherotype possess the same G serotype, the G serotypes of 488 rotavirus-positive specimens that were classified into 63 electropherotypes were determined by enzyme-linked immunosorbent assay with a supplementary use of G typing by reverse transcription-PCR. The relative frequencies over the 10-year period were 77.0 (G1), 14.5 (G2), 2.7 (G3) and 5.3% (G4), leaving the possibility that only 0.4 % had G serotypes uncommon to human rotaviruses. Of 24,050 rotaviruses extracted by reviewing 63 serotyping studies in literature, the relative frequencies of the four major G serotypes were 50.6 (G1), 9.3 (G2), 7.2 (G3) and 11.6% (G4). As to uncommon G serotypes, only 0.9% were described as serotypes other than G1-4, and our estimate for potential uncommon serotypes were at most 8.1%. Thus, both this long-term study focusing on the rotaviruses only from severe cases in a single hospital in Japan and the global review of G serotypes published to date indicate that the primary target of any rotavirus vaccines should be rotaviruses possessing serotypes G1-4.

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Detection of Rotavirus Types G8 and G10 among Brazilian Children with Diarrhea

Cited by 133 publications

References 15 publications

Full genomic analysis of a G8P[1] rotavirus strain isolated from an asymptomatic infant in Kenya provides evidence for an artiodactyl‐to‐human interspecies transmission event

Full genomic analysis of a G8P[1] rotavirus strain isolated from an asymptomatic infant in Kenya provides evidence for an artiodactyl‐to‐human interspecies transmission event

Genetic diversity of group A rotaviruses associated with repeated outbreaks of diarrhea in a farrow-to-finish farm: identification of a porcine rotavirus strain bearing a novel VP7 genotype, G26

The Relative Frequencies of G Serotypes of Rotaviruses Recovered from Hospitalized Children with Diarrhea: A 10‐Year Survey (1987–1996) in Japan with a Review of Globally Collected Data

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