2001
DOI: 10.1097/00004770-200103000-00004
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Abstract: A 16S rDNA-directed polymerase chain reaction method was used to assess the occurrence of four black-pigmented anaerobic rods, Treponema denticola, and Actinobacillus actinomycetemcomitans in acute periradicular abscesses. Pus was collected by aspiration from 10 cases diagnosed as acute abscesses of endodontic origin. DNA was extracted from the samples and analyzed using a polymerase chain reaction-based identification assay. The method allowed detecting black-pigmented anaerobes in 80% of the examined abscess… Show more

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Cited by 45 publications
(40 citation statements)
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“…The implication of this finding is not clear; while it appears to be logical that smoking cessation influences the subgingival microbiome through several different mechanisms, it is possible that P. gingivalis is metabolically more versatile than P. endodontalis. P. endodontalis is a Gramnegative, obligate anaerobe that has previously been identified in periodontal pockets as well as endodontic infections (12,21,23). This strong temporal association between smoking and the prevalence of P. endodontalis suggests that determining the role of this species in the plaque biofilm could have important implications for monitoring periodontitis in smokers.…”
Section: Discussionmentioning
confidence: 99%
“…The implication of this finding is not clear; while it appears to be logical that smoking cessation influences the subgingival microbiome through several different mechanisms, it is possible that P. gingivalis is metabolically more versatile than P. endodontalis. P. endodontalis is a Gramnegative, obligate anaerobe that has previously been identified in periodontal pockets as well as endodontic infections (12,21,23). This strong temporal association between smoking and the prevalence of P. endodontalis suggests that determining the role of this species in the plaque biofilm could have important implications for monitoring periodontitis in smokers.…”
Section: Discussionmentioning
confidence: 99%
“…In recent years, molecular methods have also been used to investigate the microbiota associated with infected root canals and with acute periapical abscesses [2,[9][10][11][12][13][14][15][16][17][18][19][20]. These methods have provided significant additional knowledge regarding the composition of the endodontic microbiota.…”
Section: Introductionmentioning
confidence: 99%
“…The Effect of Treponema denticola immunoinhibitory protein on cytokine expression in T cells Treponema denticola have been rarely found associated with endodontic diseases, however, unidentified spirochete species have now been found in endodontic infections by employing polymerase chain reaction (PCR) method 6,[15][16][17] . In this regard, Smallwood et al 18) recently reported the occurrence of spirochetes in all samples collected from infected canals using PCR.…”
Section: ⅲ Resultsmentioning
confidence: 99%
“…Likewise, Nair 4) reported that the spirochetes form a significant component of the flora of periapical specimen. Recent studies 5,6) using 16S rDNA-directed polymerase chain reaction (PCR) technique confirmed that the occurrence of Treponema denticola accounted for 52.4% of the flora in infected root canals and 50% in acute alveolar abscesses of endodontic origin. Thus, spirochetes or their byproducts may contribute to the inflammatory process and pathogenesis of the periradicular infection.…”
Section: ⅰ Introductionmentioning
confidence: 99%