Detection of protein glycation inhibitory potential of nine antidiabetic plants using a novel method

Perera, H. K. I.; Handuwalage, C. S.

doi:10.3126/ajms.v6i2.10584

Cited by 8 publications

(4 citation statements)

References 22 publications

(21 reference statements)

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“…Even though, in previous reports, the plant samples were extracted using different solvents, as opposed to the aqueous extraction in our study, these reports found that antioxidative agents contributed to anti-glycation activities, while the ethanolic extracts of P. emblica fruit collected from Thailand and Saudi Arabia exhibited high antioxidant and anti-glycation activities [ 14 , 15 ]. Similar results were observed in the methanolic extract of P. emblica fruit from Sri Lanka, with the highest anti-glycation activity among nine antidiabetic plant extracts [ 16 ]. The most abundant phenolic detected in our P. emblica , gallic acid, was also able to inhibit advanced glycation end-products in both cell cultures and animal models [ 17 , 18 , 19 ].…”

Section: Discussionsupporting

confidence: 77%

Impact of Drying Processes on Phenolics and In Vitro Health-Related Activities of Indigenous Plants in Thailand

Sirichai

Kittibunchakul

Thangsiri

et al. 2022

Plants

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Thailand has vast areas of tropical forests with many indigenous plants, but limited information is available on their phytochemical profile and in vitro inhibitions of enzymatic and nonenzymatic reactions. This study investigated phenolic profiles using liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS), antioxidant activities, and in vitro inhibitory activities of 10 indigenous plants on key enzymes related to obesity (lipase), diabetes (α-amylase and α-glucosidase), and Alzheimer’s disease (cholinesterases and β-secretase). The nonenzymatic anti-glycation reaction was also investigated. The 10 indigenous plants were Albizia lebbeck (L.) Benth, Alpinia malaccensis (Burm.) Roscoe, Careya arborea Roxb., Diplazium esculentum (Retz.) Swartz, Kaempferia roscoeana Wall., Millettia brandisiana Kurz., Momordica charantia, Phyllanthus emblica L., Zingiber cassumunar Roxb, and Zingiber citriodorum J. Mood & T. Theleide. Preparations were made by either freeze-drying or oven-drying processes. Results suggested that the drying processes had a minor impact on in vitro inhibitions of enzymatic and nonenzymatic reactions (<4-fold difference). P. emblica was the most potent antioxidant provider with high anti-glycation activity (>80% inhibition using the extract concentration of ≤6 mg/mL), while D. esculentum effectively inhibited β-secretase activity (>80% inhibition using the extract concentration of 10 mg/mL). C. arborea exhibited the highest inhibitory activities against lipase (47–51% inhibition using the extract concentration of 1 mg/mL) and cholinesterases (>60% inhibition using the extract concentration of 2 mg/mL), while Mi. brandisiana dominantly provided α-amylase and α-glucosidase inhibitors (>80% inhibition using the extract concentration of ≤2 mg/mL). Information obtained from this research may support usage of the oven-drying method due to its lower cost and easier preparation step for these studied plant species and plant parts. Furthermore, the information on in vitro inhibitions of enzymatic and nonenzymatic reactions could be used as fundamental knowledge for further investigations into other biological activities such as cell culture or in vivo experiments of these health-beneficial plants.

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Section: Discussionsupporting

confidence: 77%

Impact of Drying Processes on Phenolics and In Vitro Health-Related Activities of Indigenous Plants in Thailand

Sirichai

Kittibunchakul

Thangsiri

et al. 2022

Plants

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“…Previously we have analyzed antiglycation effects of the same extracts of nine antidiabetic plants and GL [ 14 ] using a different method in which bovine serum albumin (BSA) was taken as the model protein [ 15 ]. Our previous findings showed promising antiglycation effects in PD whole plant, PE fruit and PM latex [ 14 ]. Furthermore extracts of FR stem bark, GS leaf, MP yam and TC leaf also showed almost complete inhibition of glycation at 2 mg/ ml.…”

Section: Discussionmentioning

confidence: 99%

“…As per up-to-date literature, there are no reports on glycation induced protein cross-link inhibitory effects or antiglycation effects of FR, GS, PD and SP other than our recent report on antiglycation effects [ 14 ]. Among these four plant parts, evidence for a strong glycation induced cross-link inhibitory effect was obtained for PD (whole plant) in the present study .…”

Section: Discussionmentioning

confidence: 99%

Analysis of glycation induced protein cross-linking inhibitory effects of some antidiabetic plants and spices

Perera

Handuwalage

2015

BMC Complement Altern Med

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BackgroundProtein cross-linking which occurs towards the latter part of protein glycation is implicated in the development of chronic diabetic complications. Glycation induced protein cross-linking inhibitory effects of nine antidiabetic plants and three spices were evaluated in this study using a novel, simple, electrophoresis based method.MethodsMethanol extracts of thirteen plants including nine antidiabetic plants and three spices were used. Lysozyme and fructose were incubated at 37 °C in the presence or absence of different concentrations of plant extracts up to 31 days. Standard glycation inhibitor aminoguanidine and other appropriate controls were included. A recently established sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) method was used to detect the products of protein cross-linking in the incubation mixtures.ResultsHigh molecular weight protein products representing the dimer, trimer and tetramer of lysozyme were detected in the presence of fructose. Among the nine antidiabetic plants, seven showed glycation induced protein cross-linking inhibitory effects namely Ficus racemosa (FR) stem bark, Gymnema sylvestre (GS) leaves, Musa paradisiaca (MP) yam, Phyllanthus debilis (PD) whole plant, Phyllanthus emblica (PE) fruit, Pterocarpus marsupium (PM) latex and Tinospora cordifolia (TC) leaves. Inhibition observed with Coccinia grandis (CG) leaves and Strychnos potatorum (SP) seeds were much low. Leaves of Gymnema lactiferum (GL), the plant without known antidiabetic effects showed the lowest inhibition. All three spices namely Coriandrum sativum (CS) seeds, Cinnamomum zeylanicum (CZ) bark and Syzygium aromaticum (SA) flower buds showed cross-link inhibitory effects with higher effects in CS and SA. PD, PE, PM, CS and SA showed almost complete inhibition on the formation of cross-linking with 25 μg/ml extracts.ConclusionsMethanol extracts of PD, PE, PM, CS and SA have shown promising inhibitory effects on glycation induced protein cross-linking.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-015-0689-1) contains supplementary material, which is available to authorized users.

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“…Evidence for the b cell regeneration in the pancreas [48,49] , insulin release [50,51] , insulin like action [52,53] , increased expression of glucose transporter [54] , inhibition of digestive enzymes amylase and glucosidase (by the latex) [55,56] are some of the mechanisms identified. Furthermore, evidence on protective effects such as antioxidant effects [57] , antidyslipidaemic effects [57][58][59] and evidence for antiglycation effects of the latex are available [60,61] . Several compounds responsible for the antidiabetic effects of Pterocaipus Marsupium have been revealed.…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Pterocarpus Marsupium Importance in Various Activities - A Review

E¹

2018

IJTSRD

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Extracts of Pterocarpus Marsupium were prepared using methods like infusion, maceration, decoction and percolation. Several chemical constituents like pterostilbene, epicatechin, pterosupin, marsupin, tannins, pentosan, propterol, isoflavonoid glycol, liquiritigenin etc. were identified an Pterocarpus Marsupium Roxb. (Fabaceae) is one such herbal drug which finds its place in AYUSH. Nature has bestowed this herb with a high versatility due to which it exhibits a wide range of Pharmacological actions. Pterocarpus marsupium commonl Indian Kino tree or Asana or Vijayasar is a large deciduous tree found in the subtropical regions of the world.

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Detection of protein glycation inhibitory potential of nine antidiabetic plants using a novel method

Cited by 8 publications

References 22 publications

Impact of Drying Processes on Phenolics and In Vitro Health-Related Activities of Indigenous Plants in Thailand

Impact of Drying Processes on Phenolics and In Vitro Health-Related Activities of Indigenous Plants in Thailand

Analysis of glycation induced protein cross-linking inhibitory effects of some antidiabetic plants and spices

Pterocarpus Marsupium Importance in Various Activities - A Review

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