2011
DOI: 10.1158/1078-0432.ccr-11-0324
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Abstract: Purpose Hypermethylation of tumor suppressor gene promoters has been found in head and neck squamous carcinoma (HNSCC) and other solid tumors. We evaluated these alterations in pretreatment salivary rinses from HNSCC patients by using real-time quantitative methylation-specific PCR (Q-MSP). Experimental Design Pretreatment saliva DNA samples from HNSCC patients were evaluated for patterns of hypermethylation by using Q-MSP. Target tumor suppressor gene promoter regions were selected based on a previous study… Show more

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Cited by 78 publications
(80 citation statements)
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“…Likewise, Righini et al [22] evaluated paired tumour and saliva samples collected at diagnosis and identified a panel of six genes with frequencies of hypermethylation of 82% and 78%, respectively. Carvalho et al [16] were able to confirm an elevated rate of promoter hypermethylation detected in HNSCC patient salivary rinses by using a panel of gene promoters previously described as methylated in HNSCC but not in control subjects, by the same group [23]. Rettori et al [14] in the analysis of salivary rinse samples taken at diagnosis of HNSCC patients, five genes (CCNA1, DAPK, DCC, MGMT and TIMP3) showed high specificity and sensitivity [24].…”
Section: Introductionmentioning
confidence: 94%
See 1 more Smart Citation
“…Likewise, Righini et al [22] evaluated paired tumour and saliva samples collected at diagnosis and identified a panel of six genes with frequencies of hypermethylation of 82% and 78%, respectively. Carvalho et al [16] were able to confirm an elevated rate of promoter hypermethylation detected in HNSCC patient salivary rinses by using a panel of gene promoters previously described as methylated in HNSCC but not in control subjects, by the same group [23]. Rettori et al [14] in the analysis of salivary rinse samples taken at diagnosis of HNSCC patients, five genes (CCNA1, DAPK, DCC, MGMT and TIMP3) showed high specificity and sensitivity [24].…”
Section: Introductionmentioning
confidence: 94%
“…Body fluids can potentially carry whole cells as well as protein, DNA, and RNA that allow for detection of cellular alterations related to cancer. Examples of relevant body fluids used for detection include analysis of sputum for lung cancer diagnosis [9,10], urine for urologic tumours [11,12], saliva for HNSCC [13][14][15][16], breast fluid [17], as well as serum or plasma for almost all types of cancer [18][19][20]. The detection of DNA methylation in body fluids such as saliva is a non-invasive technique that can easily obtain epithelial cells shed from the mucosal lining of the mouth and throat.…”
Section: Introductionmentioning
confidence: 99%
“…Curiously, several investigations are now reporting saliva-based genomic methylation analyses discerning oral squamous cell carcinoma (OSCC) (111) and head and neck squamous cell carcinoma (HNSCC) patients from their respective controls (112,113). Additionally, a number of studies have explored local and global epigenetic alterations with regard to age (114,115,116), suggesting the possibility of saliva-based predictive screenings for age-related diseases.…”
Section: Methylomicsmentioning
confidence: 99%
“…The methylation results obtained by QMSP in our analysis were classified to give a binary status, in which any quantity of methylation in a sample was considered positive. 25 …”
Section: Hpv16 Detection By Means Of Quantitative Polymerase Chain Rementioning
confidence: 99%