Detection of P-Glycoprotein With Four Monoclonal Antibodies in Normal and Tumor Tissues

In-vitro respiratory drug absorption models possess nominal functional P-glycoprotein activity

“…The low efflux ratio produced by the respiratory cell models was consistent with NHBE and Calu‐3 cells expressing P‐gp to a lower extent than the carcinoma cell line, Caco‐2 5,31,32 . In Calu‐3 cells, the digoxin transport was polarised in the B–A direction, suggesting the presence of P‐gp in the apical cell membrane after 21 days in culture, a result that concorded with the data generated by Hamilton et al 13 .…”

“…The relatively low levels of P‐gp expression in the lung compared with those in other tissues has been well documented 5,31,32 . This study has shown that although there was evidence of nominal P‐gp activity in respiratory cells in vitro , this was not found in the ex‐vivo lung and was at a much lower level compared with P‐gp activity in the intestinal epithelial cells.…”

P-glycoprotein Expression Is Upregulated in a Pre-Clinical Model of Traumatic Brain Injury

“…7,9,10,[30][31][32][33][34][35][36][37][38][39] Most of these studies have used commercial Pgp antibodies. Similar to that reported previously for other Pgp antibodies, [40][41][42][43][44] our validation results using KO mice revealed that some tested antibodies had poor specificity, and caution must be taken when interpreting results of studies using these antibodies.…”

In-vitro respiratory drug absorption models possess nominal functional P-glycoprotein activity

“…The low efflux ratio produced by the respiratory cell models was consistent with NHBE and Calu-3 cells expressing P-gp to a lower extent than the carcinoma cell line, Caco-2. [5,31,32] In Calu-3 cells, the digoxin transport was polarised in the B-A direction, suggesting the presence of P-gp in the apical cell membrane after 21 days in culture, a result that concorded with the data generated by Hamilton et al [13] In contrast, the efflux observed in NHBE cells appeared to result from active transport at the basolateral plasma membrane. This was in contrast to the conclusions of Lin et al [16] , who reported apical membrane localisation based on their observations of nonspecific inhibition of secretory efflux.…”