Detection of OXA-48 Carbapenemase in the Pandemic Clone Escherichia coli O25b:H4-ST131 in the Course of Investigation of an Outbreak of OXA-48-Producing Klebsiella pneumoniae

Morris, Dearbháile; McGarry, Edel; Cotter, Michael; Passet, Virginie; Lynch, Maureen; Ludden, Catherine; Hannan, Margaret M.; Brisse, Sylvain; Cormican, Martin

doi:10.1128/aac.00638-12

Cited by 32 publications

(25 citation statements)

References 17 publications

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“…This time, we observed at the beginning that the majority of cells no grow, but a small proportion of cells survived up to form a new colony. The key signature of persistence is the biphasic kill curve obtained when bacteria in batch culture are exposed to a bactericidal antibiotic: the killing rate is initially very high but then slows and may even level off to zero [17]. This phenomenon was first described in the 1940s, when Staphylococcal infections were seen to recur, even after extensive treatment with high doses of penicillin [36].…”

Section: Discussionmentioning

confidence: 99%

“…Numerous factors have been proposed as being responsible for the persistence phenotype occurring independently of stable genetic changes [39]. Recent research has shown that persistence of the model organism Escherichia coli depends on toxin-antitoxin (TA) loci [17,39,40,41] where the overproduction of toxin encoded by TA loci induces a persistence-like state from which the cells could be resuscitated by the induction of antitoxin gene transcription. A better understanding of the mechanisms behind bacterial persistence may lead to the design of drugs to combat chronic and recurrent infections, such as recurrent urinary infections.…”

Section: Discussionmentioning

confidence: 99%

“…The antibiotic resistance is acquired through genetic changes and may be an important factor to antibiotic therapy failure, however, the presence of a subpopulation of multi-drug tolerance (MDT) bacteria could be other important cause of infections fail to clear despite of the absence of resistance organisms encouraging the recurrent urinary infections (recurrence is used for repeatedly occurring UTI without knowledge of strain similarity), such bacteria (persister cells) are typically thought to represent nongrowing, isogenic, physiological variants that gain MDT via the inactivation of antibiotictargeted cellular processes through presumed metabolic dormancy [16,17]. In this current study, genetic and phenotypic characterization of a group of UPEC strains isolated from Mexican patients with cystitis and prostatitis was carried out.…”

Section: Introductionmentioning

confidence: 99%

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UPEC strain characterization isolated from Mexican patients with recurrent urinary infections

Morales-Espinosa

Hernández‐Castro

Delgado

et al. 2016

J Infect Dev Ctries

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Introduction: Any microorganism is capable of causing urinary tract infections (UTI). However, Uropathogenic Escherichia coli (UPEC) is responsible for the majority of UTI. A variety of virulence genes have been identified in UPEC. Diverse epidemiological studies support that specific subsets of genes are characteristic of each E. coli uropathogenic subtype involved in the development of cystitis, pyelonephritis, and urosepsis. Methodology: Twenty-three E. coli strains isolated from women with cystitis, 25 from men with prostatitis were characterized according to serotype, virulence genes, PFGE profile and susceptibility antimicrobials. Results: E. coli O25:H4-ST131 was more frequently isolated from cystitis than prostatitis. The majority of cystitis strains presented almost all the virulence genes, contrary to 60% of prostatitis strains. The strains characterized by serology, PFGE and MLST and virulence profile showed that cystitis strains shared a lineage with their own genomic fingerprint, indicating that these strains derived from a common ancestor. The prostatitis strains showed serotypes diverse and much higher degree of genetic diversity, indicating that they are an unrelated group. More than 50% of isolates were resistant to at least 7 of the antimicrobials tested. Antibiotic-tolerant cells were observed, presenting with more frequency in response chloramphenicol, tetracycline, trimethoprim-sulfometoxazole and dicloxicillin. Conclusions: Persister cells can be detected from the beginning of the infection. The importance of these persister cells that exhibit multidrug tolerance is that a single surviving bacterium can initiate again an infection making it clinical relevant in the chronic urinary tract infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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UPEC strain characterization isolated from Mexican patients with recurrent urinary infections

Morales-Espinosa

Hernández‐Castro

Delgado

et al. 2016

J Infect Dev Ctries

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“…The class C enzymes detected include CMY-2 (19,103) and CMY-4 (87), with DHA-1 described only rarely (19). Finally, Ambler class D ␤-lactamases, such as OXA-48, have only rarely been found in ST131 isolates (104,105). Two narrow-spectrum ␤-lactamases, OXA-1 and TEM-1, have frequently been found in ST131 isolates, generally in association with CTX-M-15 enzymes, following plasmid transfer (see "Characterization of plasmids harbored by clinical isolates of E. coli ST131" above) (9,55,58,62,94,106).…”

Section: Antibiotic Resistancementioning

confidence: 99%

Escherichia coli ST131, an Intriguing Clonal Group

2014

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SUMMARY In 2008, a previously unknown Escherichia coli clonal group, sequence type 131 (ST131), was identified on three continents. Today, ST131 is the predominant E. coli lineage among extraintestinal pathogenic E. coli (ExPEC) isolates worldwide. Retrospective studies have suggested that it may originally have risen to prominence as early as 2003. Unlike other classical group B2 ExPEC isolates, ST131 isolates are commonly reported to produce extended-spectrum β-lactamases, such as CTX-M-15, and almost all are resistant to fluoroquinolones. Moreover, ST131 E. coli isolates are considered to be truly pathogenic, due to the spectrum of infections they cause in both community and hospital settings and the large number of virulence-associated genes they contain. ST131 isolates therefore seem to contradict the widely held view that high levels of antimicrobial resistance are necessarily associated with a fitness cost leading to a decrease in pathogenesis. Six years after the first description of E. coli ST131, this review outlines the principal traits of ST131 clonal group isolates, based on the growing body of published data, and highlights what is currently known and what we need to find out to provide public health authorities with better information to help combat ST131.

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“…[3,4] Further treatment options are limited for this group because there were reports of carbapenemresistant ST 131 strains. [5] Can et al (2014) observed that resistance rates were above 25% against ciprofloxacin, TMP-SMX, and cefuroxime in their 294 isolates from acute cystitis cases. Treatment failure was three times more common in the group infected with ST131 than other groups of E.coli.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Wang

2016

AJP

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Background: Escherichia coli sequence type 131 (ST131) has emerged among bacteria causing urinary tract infection (UTI) in the previous decade. This ST contains multiple drug resistant (MDR) genes together with genes encoding many virulence factors. As a result, this strain of uropathogenic E. coli (UPEC) gives rise to treatment failure with consequent prolonged stay in a hospital. Therefore, earlier identification of this strain in the hospital has advantage in combating severe type of UTI. Objective: To detect ST 131 strains in MDR UPEC isolates from two hospitals of Sabah. Materials and Methods: Antibiotic susceptibility tests were performed to detect MDR isolates. Two polymerase chain reactions (PCRs) including mdh and gyrB allelic-specific PCR were performed on these MDR isolates to detect ST131 strains. Results: The results showed four isolates were resistant to TMP-SMX, gentamycin, ciprofloxacin, and cefotaxime, and three isolates of these were investigated to be ST131 strains by two PCR reactions. Conclusion: There is the presence of ST131 strains in hospitals of Sabah. This information will be a guideline for the clinician in the management of UTI in the clinical settings.

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Detection of OXA-48 Carbapenemase in the Pandemic Clone Escherichia coli O25b:H4-ST131 in the Course of Investigation of an Outbreak of OXA-48-Producing Klebsiella pneumoniae

Cited by 32 publications

References 17 publications

UPEC strain characterization isolated from Mexican patients with recurrent urinary infections

UPEC strain characterization isolated from Mexican patients with recurrent urinary infections

Escherichia coli ST131, an Intriguing Clonal Group

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