Detection of Neospora caninum in an aborted goat foetus

Eleni, Claudia; Crotti, Silvia; Manuali, Elisabetta; Costarelli, S.; Filippini, G.; Moscati, Livia; Magnino, Simone

doi:10.1016/j.vetpar.2004.06.017

Cited by 42 publications

(25 citation statements)

References 10 publications

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“…Pregnant goats have been susceptible to experimental infection, at which subsequently aborted infected progeny (Lindsay et al 1995). Miscarriages and stillborn kids with confirmed presence of the parasite in their brain have been reported in goats (Dubey et al 1992;Eleni et al 2004). Although antibodies to N. caninum have been observed in human sera (Petersen et al 1999) the parasite has not been detected in human tissues, therefore, its transmission to humans is still debatable.…”

Section: Introductionmentioning

confidence: 99%

Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia

Čobádiová

Reiterová

Derdáková³

et al. 2013

Acta Parasitologica

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Parasitic diseases of livestock together with poor welfare conditions can negatively affect the health status and production of small ruminants. Protozoan parasites and tick-borne infectious agents are common threat of livestock including small ruminants mostly during the pasture season. Therefore the priority of the study was to analyse the circulation and presence of two protozoan parasites Toxoplasma gondii and Neospora caninum as well as tick-transmitted bacterium Anaplasma phagocytophilum in one selected goat farm in Eastern Slovakia. Throughout a three-year study period we have repeatedly screened the sera and blood of goats and dogs from monitored farm. In total, 343 blood serum samples from 116 goats were examined by ELISA. The mean seropositivity for T. gondii was 56.9% (66/116, CI (95%) = 48-66.0) and 15.5% (18/116, CI (95%) = 9.3-22.7) for N. caninum. The permanent occurrence of anti-Toxoplasma and anti-Neospora antibodies was detected in repeatedly examined goats during the whole monitored period. The presence of both parasites in the flock was analysed by PCR. DNA of T. gondii was confirmed in 12 out of 25 Toxoplasma-seropositive goats and N. caninum in 14 samples out of 18 Neospora-seropositive animals; four goats were co-infected with both pathogens. The risk of endogenous transmission of both parasites was pursued by examination of 41 kid's sera, where seropositivity for toxoplasmosis was 31.7% and for neosporosis 14.6%. In dogs 61.1% seropositivity for T. gondii and 38.9% for N. caninum was found, however, their faeces were negative for coccidian oocysts. Eight out of 108 tested animals were infected with A. phagocytophilum, the causative agent of tick-borne fever. Seven of them were simultaneously infected with T. gondii and A. phagocytophilum, out of which four goats were concurrently infected with all three pathogens.

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Section: Introductionmentioning

confidence: 99%

Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia

Čobádiová

Reiterová

Derdáková³

et al. 2013

Acta Parasitologica

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“…described brain hemorrhage. In goat fetuses, Eleni et al (2004) observed nonsuppurative meningoencephalitis and multifocal necrosis associated with N. caninum cysts in the cortex area. Histological consequences of N. caninum infection in heart and brain of cattle fetuses are similar to those described for the sheep fetuses investigated in present study (Corbelliniet al 2000, Pescador et al 2007, Cabral et al 2009).…”

Section: Resultsmentioning

confidence: 87%

“…As reported for goats (Eleni et al 2004), clinical cases associated to natural infection of sheep by Neospora caninum are uncommon, with a few abortion cases , Jolley et al 1999) and congenital disorders (Pena et al 2007) described. However, several serological surveys conducted in different countries, including Brazil, showed that the infection is disseminated in this species (Figliuolo et al 2004, Salaberry et al 2010, as well as in other animal species , Teixeira et al 2010, Toscan et al 2010, Camillo et al 2011, Varaschin et al 2011.…”

Section: Discussionmentioning

confidence: 95%

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Sheep abortion associated with Neospora caninum in Mato Grosso do Sul, Brazil

et al. 2012

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Pesq. Vet. Bras. 32(8) Canids are the main hosts of Neospora caninum, but cattle, (sheep, goats and horses may serve as intermediary hosts. N. caninum infection of pregnant intermediary hosts may provoke abortion and neonatal infections. This study is the ϐirst to report lamb abortion associated with N. caninum in Mato Grosso do Sul. Epidemiological data were obtained from interviews with sheep producers. For microscopic examination, fragments of different organs removed from 4 sheep fetuses, aborted and necropsied, were ϐixed in 10% formaldehyde, embedded in parafϐin and subjected to the hematoxylin-eosin staining protocol and immunohistochemistry (IHC) to test for N. caninum and Toxoplasma gondii. The abortion outbreak studied was reported from a herd of 268 Santa Inês sheep (including 186 pregnant ewes), with 10 abortion cases in the last third of gestation. Four fetuses were examined, 3 from a same ewe. At necropsy, one fetus exhibited crackling in the lung and all its organs were reddish. Histological ϐindings detected mononuclear cell inϐiltrates among myocardium ϐibers and around blood vessels, in addition to circular structures with basophilic points resembling protozoans. IHC tests revealed strongly positive staining for N. caninum and weakly positive for T. gondii, characterizing N. caninum infection.

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“…However, there are reports of abortion in sheep naturally infected by N. caninum in Japan (Kobayashi et al 2001), Switzerland (Hässig et al 2003), Slovakia (Spilovská et al 2009), Spain (Moreno et al 2012, González--Warleta et al 2014 and New Zealand (Howe et al 2012). There are also reports of abortion in goats naturally infected by N. caninum in the United States (Barr et al 1992, Costa Rica (Dubey et al 1996), Italy (Eleni et al 2004), Spain (Moreno et al 2012) and Argentina (Unzaga et al 2014). In Brazil, N. caninum congenital infection in goats and sheep has been described in the South, Southeast and Midwest regions, with no descriptions in the other regions of the country (Corbellini et al 2001, Pinto et al 2012, Varaschin et al 2012, Mesquita et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Transplacental transmission of Neospora caninum in naturally infected small ruminants from northeastern Brazil

et al. 2017

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RESUMO.-[Transmissão transplacentária de Neospora caninum em pequenos ruminantes infectados naturalmente do Nordeste do Brasil.] Toxoplasma gondii e Neospora caninum são reconhecidos como protozoário causadores de aborto em ovinos e caprinos. Desta forma, objetivou-se descrever a transmissão transplacentária desses agentes em pequenos ruminantes na região Nordeste do Brasil. Foram examinados seis fetos caprinos e onze fetos ovinos, totalizando 78 amostras de diferentes tecidos (cé-rebro, fígado, pulmão, rim e coração) provenientes de propriedades rurais com histórico de aborto. As amostras foram analisadas por Reação em Cadeia da Polimerase (PCR), Histopatologia (HP) e Imunohistoquímica (IHQ), com a finalidade de associar o abortamento a T. gondii e/ou N. caninum. Nenhuma amostra foi positiva na PCR e IHQ para T. gondii. Algumas amostras de cérebro, fígado, pulmão, rim e coração de fetos de caprinos e ovinos foram positivas na PCR para N. caninum. Na histopatologia foi observado leve infiltrado mononuclear no fígado e necrose com calcificação no SNC de um caprino, associada à imunomarcação positiva para N. caninum na IHQ e PCR positiva. Os resultados confirmam a transmissão vertical de N. caninum em caprinos naturalmente infectados na região nordeste do Brasil. Toxoplasma gondii and Neospora caninum are causative agents of abortion in sheep and goats. Thus, the present study aimed to describe the transplacental transmission of these protozoans in small ruminants of northeastern Brazil. Seventeen fetuses (6 goats and 11 sheep) from farms with history of abortion were necropsied and samples were collected from different tissues (brain, liver, lung, kidney and heart). The samples were analyzed by PCR, histopathology (HP) and immunohistochemistry (IHC) to evaluate whether T. gondii and/ or N. caninum infection were the cause of abortion. None of the samples was positive for T. gondii according to PCR and IHC results. Some brain, liver, lung, kidney and heart samples of goat fetuses were positive for N. caninum by PCR. In the histopathology, mild mononuclear infiltration and necrosis with calcification were observed in the liver and brain of one goat fetus, respectively, that also was positive for N. caninum by PCR and IHC. The results confirmed vertical transmission of N. caninum in naturally infected goats of northeastern, Brazil.

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Detection of Neospora caninum in an aborted goat foetus

Cited by 42 publications

References 10 publications

Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia

Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia

Sheep abortion associated with Neospora caninum in Mato Grosso do Sul, Brazil

Transplacental transmission of Neospora caninum in naturally infected small ruminants from northeastern Brazil

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