Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods

Suffys, Philip Noël; Rocha, Adalgiza da Silva; Brandão, Adeílton; Vanderborght, Bart; Mijs, Wouter; Jannes, Geert; Mello, Fernanda C. Q.; Pedro, Heloisa da Silveira Paro; Fonseca, Leila de Souza; Oliveira, Rosângela Siqueira de; Leão, Sylvia Cardoso; Saad, Maria Helena Féres

doi:10.1099/jmm.0.46218-0

Cited by 16 publications

(12 citation statements)

References 23 publications

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“…But there was no statistical significance between the Groups II and III. A similar report was given by Mrudula Patel et al .,[19] Similar observations with variable percentage of positivity were also reported from Thailand (adults 66%; children 70%); Hongkong (54.8%), Italy (64%) and India (65.3%) by Teanpaisan and Nittyanantha;[16] Samarnayake et al . ;[17] Campo et al .…”

Section: Discussionsupporting

confidence: 83%

“…[14] Mrudula Patel et al ., reported 78.6% of C. albicans as the most common species isolated and 21.4% of nonalbicans species. [19] In the present study, an attempt is made to know the prevalence of Candida in HIV positive individuals, as immune-suppression is always associated with the disease. CD4 count is a marker of immunosuppression and also helps in prognosticating the case.…”

Section: Discussionmentioning

confidence: 99%

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Isolation, identification, and carriage of candidal species in PHLAs and their correlation with immunological status in cases with and without HAART

Kantheti

Reddy

Ravikumar

et al. 2012

J Oral Maxillofac Pathol

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Aims and Objectives:To know the prevalence of Candidal colonization, and to isolate and know the Candidal species prevalent in the oral cavity from the oral rinse samples collected from the individuals attending to the Voluntary Counseling and Confidential Testing Center (VCCTC) and the antiretro-viral therapy (ART) Center at Government General Hospital, Guntur, Andhra Pradesh, South India.Materials and Methods:The study group consisted of 50 HIV negative asymptomatic individuals (Group I); 50 HIV positive individuals (people living with HIV AIDS [PLWHA's]), who are naïve to antiretro-viral therapy (direct walk-in clients of VCCTC) (Group II); and 50 HIV positive individuals with CD4+ count less than 250 and who are started on highly active anti retroviral therapy (HAART) (Group III). Routine mycological tests for the isolation of pure cultures of Candida and also the speciation procedures were done.Results:In the study group, 53 (Group I=11; Group II=23; Group III=19) were culture positive. The prevalence of Candida was comparatively high in the age range between 41–50 years in Group II; 51–60 years, in Group III. A male predominance was observed in the Group I (M:F=16:6) and Group III (M:F=20:18), with a slight female predominance in the Group II (F:M=24:22). The overall culture positivity was 35.3%. Candida albicans was the highest prevalent species (47.17% of the isolates). A comparison of the culture positivity with the CD4 counts of the study subjects was statistically highly significant. A pair wise comparison of the culture positivity with that of the colony forming units/mL from the subjects showed a high significance between Group I and Group II, and between Group I and Group III.Conclusion:Candidal infections in immuno compromised patients are often severe, rapidly progressive, and difficult to treat and such patients have a definitive risk of developing oral candidiasis wherein, even the members of the normal oral flora may become pathogenic. Candida albicans is the common isolate. Nonalbicans species are also emerging as opportunistic pathogens. In view of this changing pattern, it is strongly recommended that species identification can help in much better treatment strategies, and thus, gain a good control over the disease. The findings of this study would be helpful in any further studies which, if done prospectively on a larger cohort, can be confirmatory.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Isolation, identification, and carriage of candidal species in PHLAs and their correlation with immunological status in cases with and without HAART

Kantheti

Reddy

Ravikumar

et al. 2012

J Oral Maxillofac Pathol

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“…Certainly in some patients multiple NTM can grow at the same or different times, and M. lentiflavum may be no different in this respect. M. lentiflavum has been cultured from sputum containing MAC and from sputum containing M. tuberculosis , but these cases may represent colonization/contamination rather than infection ( 23 , 28 ). Concurrent isolation of M. lentiflavum and S. aureus , which probably represents contamination or colonization, has not been reported as far as we are aware.…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium lentiflavumin Drinking Water Supplies, Australia

Marshall¹,

Carter²,

Torbey³

et al. 2011

Emerg. Infect. Dis.

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“…Molecular typing methods have permitted investigation of the outbreaks [36, 61, 100, 139, 165, 166, 213, 214], discrimination between exogenous reinfection and endogenous reactivation [209, 210, 215–217], identification of mixed infections [215, 218, 219], and cases of misdiagnosis due to laboratory contamination [220–223]. Molecular markers have also extensively been used for tracking transmission patterns within specific populations and/or defined geographical settings [106–108, 153, 206, 224–227].…”

Section: Discussionmentioning

confidence: 99%

Current Methods in the Molecular Typing ofMycobacterium tuberculosisand Other Mycobacteria

Jagielski

Ingen

Rastogi

et al. 2014

BioMed Research International

121

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In the epidemiology of tuberculosis (TB) and nontuberculous mycobacterial (NTM) diseases, as in all infectious diseases, the key issue is to define the source of infection and to disclose its routes of transmission and dissemination in the environment. For this to be accomplished, the ability of discerning and tracking individual Mycobacterium strains is of critical importance. Molecular typing methods have greatly improved our understanding of the biology of mycobacteria and provide powerful tools to combat the diseases caused by these pathogens. The utility of various typing methods depends on the Mycobacterium species under investigation as well as on the research question. For tuberculosis, different methods have different roles in phylogenetic analyses and person-to-person transmission studies. In NTM diseases, most investigations involve the search for environmental sources or phylogenetic relationships. Here, too, the type of setting determines which methodology is most suitable. Within this review, we summarize currently available molecular methods for strain typing of M. tuberculosis and some NTM species, most commonly associated with human disease. For the various methods, technical practicalities as well as discriminatory power and accomplishments are reviewed.

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Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods

Cited by 16 publications

References 23 publications

Isolation, identification, and carriage of candidal species in PHLAs and their correlation with immunological status in cases with and without HAART

Isolation, identification, and carriage of candidal species in PHLAs and their correlation with immunological status in cases with and without HAART

Mycobacterium lentiflavumin Drinking Water Supplies, Australia

Current Methods in the Molecular Typing ofMycobacterium tuberculosisand Other Mycobacteria

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