Detection of lipoglycans in ureaplasmas

Abstract

Serotypes 3, 4, and 8 of Ureaplasma urealyticum were found to contain lipoglycans. Although the ratios of their components differed, all contained neutral sugars, fatty acids, glycerol, and phosphorus. All three became labeled when the organisms were cultivated in the presence of [14C]glucose, [14C]palmitic or [14C]oleic acids, and inorganic 32p. Only neutral sugars were found, and these consisted of mannose, glucose, and galactose. Hot phenol extracts of uninoculated and supernatant culture media contained polymeric carbohydrate, but this differed in composition from ureaplasmal lipoglycans and did not become radiolabeled. Since lipoglycans contained phosphorus but no amino sugars, they could be separated from contaminating polysaccharides by anion exchange chromatography.

Intrauterine Ureaplasma is associated with small airway obstruction in extremely preterm infants

“…Ureaplasma may activate the complement system in situ. Ureaplasm a lipoglycans consist of mannose residues 26 . Benstein et al 27 reported Ureaplasma parvum attached to mannose‐binding lectin.…”

Metabolomics in premature labor: A novel approach to identify patients at risk for preterm delivery

“…Several lines of evidence support this view: 1) a low amniotic fluid glucose concentration is a well-established biomarker of IAI. [134][135][136][137] Low glucose concentrations are also associated with microbial invasion of other biological fluids including cerebrospinal [138,139] and synovial fluid; [140] 2) Fusobacterium species contain a galactose-binding protein, and it is possible that this protein may contribute to the decrease concentrations of galactose in infected amniotic fluid; [141] 3) Ureaplasmal lipoglycans are constituted primarily of mannose, glucose, and galactose, [142] suggesting that these carbohydrates may be consumed with the replication of this microorganism in the amniotic fluid; and 4) glucose is also required as a fuel by activated neutrophils engaged in the process of microbial phagocytosis and killing. [143] Methyladenine was the most important classifier as determined by Random Forest analysis (Gini Index).…”

Identification of two glycosylated components of Mycoplasma penetrans: a surface-exposed capsular polysaccharide and a glycolipid fraction

“…In preliminary experiments using a standard procedure for lipoglycan purification (Smith, 1985(Smith, , 1992 no glycoconjugate could be extracted from M. penetrans cells with hot phenol after removal of lipids. All the glycosylated material was found in the methanol/ chloroform phase used for removal of lipids (data not shown).…”