Detection of Leishmania-specific DNA and surface antigens using a combination of functionalized magnetic beads and cadmium selenite quantum dots

Ανδρεάδου, Μαργαρίτα; Liandris, Emmanouil; Gazouli, Maria; Ματαράγκα, Αντωνία; Tachtsidis, Ilias; Goutas, Nikolaοs; Vlachodimitropoulos, Dimitrios; Ikonomopoulos, John

doi:10.1016/j.mimet.2015.11.019

Cited by 29 publications

(16 citation statements)

References 28 publications

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“…Advantageously, in the present work, labeling of Leishmania parasites was achieved in suspension, which allows casein–AuNPs conjugates to interact readily and rapidly with GP63 proteins overexpressed at the surface of the parasite. GP63 proteins were previously used as a recognition element in the design of sensing assays for Leishmania parasites [42,43].…”

Section: Resultsmentioning

confidence: 99%

Casein-Conjugated Gold Nanoparticles for Amperometric Detection of Leishmania infantum

et al. 2019

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Sensitive and reliable approaches targeting the detection of Leishmania are critical for effective early diagnosis and treatment of leishmaniasis. In this frame, this paper describes a rapid quantification assay to detect Leishmania parasites based on the combination of the electrocatalytic ability of gold nanoparticles (AuNPs) to act as a catalyst for the hydrogen formation reaction along with the specificity of the interaction between casein and the major surface protease of the Leishmania parasite, GP63. First, pure and casein-modified AuNPs were prepared and characterized by scanning electron microscopy and ultraviolet–visible spectroscopy. Then, casein-conjugated AuNPs were incubated with Leishsmania parasites in solution; the formed complex was collected by centrifugation, treated by acidic solution, and the pelleted AuNPs were placed on screen-printed carbon electrodes (SPCEs) and chronoamperometric measurements were carried out. Our results suggest that it is possible to detect Leishmania parasites, with a limit less than 1 parasite/mL. A linear response over a wide concentration interval, ranging from 2 × 10−2 to 2 × 105 parasites/mL, was achieved. Additionally, a pretreatment of Leishmania parasites with Amphotericin B, diminished their interaction with casein. This findings and methodology are very useful for drug efficacy assessment.

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Section: Resultsmentioning

confidence: 99%

Casein-Conjugated Gold Nanoparticles for Amperometric Detection of Leishmania infantum

et al. 2019

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“…Nanostructured films with Leishmania amazonensis and Trypanosoma cruzi-specific antigen through impedance spectroscopy have been utilized as a detection method for efficient diagnosis of leishmaniasis [26]. Andreadou et al utilized a combination of cadmium selenite quantum dot and magnetic beads as probe for detection of Leishmania DNA and its specific surface antigens [27]. Magnetic beads capture the analytes from the solution, and cadmium selenite quantum dots help in detection of specific molecule of interest, that is, Leishmania DNA and proteins with 100% sensitivity and specificity in 55 cultured isolates of various microbial pathogens having a lower limit of 3125 ng/μl and 103 cells/ml for Leishmania protein and DNA.…”

Section: Nanoparticle-based Diagnostics For Vlmentioning

confidence: 99%

“…For this, several key features such as sensitivity, specificity, speed, accuracy, accessibility, ease of usage and field applicability beside their cost-effectiveness, versatility and discriminatory capacity should be considered for detection and quantification of 21 Leishmania species that are pathogenic to humans [2]. Although, the progress of nanodiagnostics in case of VL remains at a snail pace with prime focus on DNA/RNA-based detections using inorganic NPs using gold, cobalt-zinc ferrite and cadmium selenide quantum dots have sparked a ray of hope for betterment in near feature for the detection of parasite [27][28][29]31] and much of the focus has to be shifted toward proteomic and glycomic approaches. In near future, NPs having the ability for simultaneous detection of leishmaniasis and its drug resistance would be a great addition for the present line of diagnosis.…”

Section: Nanodiagnostic Developmentmentioning

confidence: 99%

Envisioning the Innovations in Nanomedicine to Combat Visceral Leishmaniasis: For Future Theranostic Application

Singh

Gedda

Mudavath

et al. 2019

Nanomedicine (Lond.)

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Visceral leishmaniasis (VL) is a life-threatening parasitic disease affecting impoverished people of the developing world; and much effort has been spent on the early case detection and treatment. However, current diagnostics and treatment options are not sufficient for appropriate surveillance in VL elimination setting. Hence, there is a dire need to develop highly sensitive diagnostics and less toxic effective treatments for proper management of cases and to achieve the sustained disease elimination. Although, promising results have been observed with nanomedicines in leishmaniasis; there are great challenges ahead especially in translating this to clinical setting. This review provides updated progress of nanomedicines in VL, and discussed how these innovations and future directions play vital role in achieving VL elimination.

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“…In order to synthesize heterodimer structures, nonmetallic magnetic particles were frequently utilized, but so far only a few plasmonic components with nonmetallic nature were employed. The main class of nonmetallic plasmonic nanomaterials is quantum dots (QDs) with numerous functionalities for biomedical applications, such as drug delivery, especially for small molecules, like 5-FU [125,126], biosensing [127,128,129,130], and multimodal imaging [131,132]. Since quantum dots normally show insufficient biocompatibility and colloidal stability, their combination with nonmetallic magnetic nanoparticles could be helpful to overcome drawbacks.…”

Section: Structure Designmentioning

confidence: 99%

Magnetic-Plasmonic Heterodimer Nanoparticles: Designing Contemporarily Features for Emerging Biomedical Diagnosis and Treatments

2019

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Magnetic-plasmonic heterodimer nanostructures synergistically present excellent magnetic and plasmonic characteristics in a unique platform as a multipurpose medium for recently invented biomedical applications, such as magnetic hyperthermia, photothermal therapy, drug delivery, bioimaging, and biosensing. In this review, we briefly outline the less-known aspects of heterodimers, including electronic composition, interfacial morphology, critical properties, and present concrete examples of recent progress in synthesis and applications. With a focus on emerging features and performance of heterodimers in biomedical applications, this review provides a comprehensive perspective of novel achievements and suggests a fruitful framework for future research.

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Detection of Leishmania-specific DNA and surface antigens using a combination of functionalized magnetic beads and cadmium selenite quantum dots

Cited by 29 publications

References 28 publications

Casein-Conjugated Gold Nanoparticles for Amperometric Detection of Leishmania infantum

Casein-Conjugated Gold Nanoparticles for Amperometric Detection of Leishmania infantum

Envisioning the Innovations in Nanomedicine to Combat Visceral Leishmaniasis: For Future Theranostic Application

Magnetic-Plasmonic Heterodimer Nanoparticles: Designing Contemporarily Features for Emerging Biomedical Diagnosis and Treatments

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