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Cited by 35 publications
(40 citation statements)
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References 27 publications
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“…The somewhat higher sensitivity of ISH-SP in spite of the slightly lower signal intensity compared to that of ISH-GP may be due to the better signal-to-noise ratio of ISH-SP. The use of ISH and IHC increased the number of correctly diagnosed positive cases in comparison to the results for HP similarly to what has been reported by other authors when using IHC (16,18,28,29).…”
Section: Discussionsupporting
confidence: 86%
See 2 more Smart Citations
“…The somewhat higher sensitivity of ISH-SP in spite of the slightly lower signal intensity compared to that of ISH-GP may be due to the better signal-to-noise ratio of ISH-SP. The use of ISH and IHC increased the number of correctly diagnosed positive cases in comparison to the results for HP similarly to what has been reported by other authors when using IHC (16,18,28,29).…”
Section: Discussionsupporting
confidence: 86%
“…Therefore, a variety of serological, parasitological, and molecular laboratory methods have been developed to detect infection by L. infantum in dogs (10,11). Serological assays, PCR, and parasitological culture are the most sensitive methods for the diagnosis of L. infantum infection (12)(13)(14)(15)(16)(17)(18). However, these three methods do not allow visualization of the intact amastigotes within the tissue and correlation of the parasites with associated lesions, which is possible by histopathology (HP) and immunohistochemistry (IHC) (19).…”
mentioning
confidence: 99%
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“…5,104 Histopathology with hematoxylin and eosin is a valuable diagnostic tool in doubtful cases, although the identification of amastigotes is more difficult than with Giemsa cytology. 34,129 Granulomatous inflammation has been associated with higher cutaneous parasitism compared to the other types of dermatitis and regardless of its pattern. 39 To diagnose CanL, the presence of amastigotes should be combined with compatible microscopic lesions, although the opposite can also be true.…”
Section: General Pathomechanismsmentioning
confidence: 99%
“…129 Finally, polymerase chain reaction and in situ hybridization may be used, even in paraffin-embedded blocks, because of their higher specificity and sensitivity. 34,89,151 Organ-Specific Pathomechanisms…”
Section: General Pathomechanismsmentioning
confidence: 99%