Detection of koi herpesvirus (KHV) and carp oedema virus (CEV) in invasive round goby, <i>Neogobius melanostomus</i> Pallas, 1814, from Poland and Germany

Jin, Yeonhwa; Adamkowska, Natalia; Kiełpińska, Jolanta; Bergmann, Sven

doi:10.2478/jvetres-2020-0037

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…Some farms from Central Java and Lampung showed both CyHV-3-negative and positive (with a single melting peak) fish. The negative CyHV-3 results can probably be explained by a low level of infection, as already observed (Monaghan et al 2015;Jin et al 2020). In contrast, farms from Lampung Bay showed variable levels of CyHV-3 infection, even between very close farms.…”

Section: Resultssupporting

confidence: 65%

Prevalence of Cyprinid herpesvirus 3 and ORF150 genomic variations in carp populations of Indonesia

Fuandila¹,

Lusiastuti²,

Yuhana

et al. 2023

Bulletin of the European Association of Fish Pathologists

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Cyprinid herpesvirus-3 (CyHV-3) is the etiological agent of koi herpesvirus disease (KHVD) in common and koi carp farming. This highly contagious pathogen has a 295-kb genome that harbours 156 open reading frames. Recent in vitro experimental evolution studies highlighted strong dynamics of genomic structural variations (SVs), in particular in the region of ORF150, an ORF potentially involved in virus multiplication and host inflammatory response. Among these SVs, a 1363-bp deletion could be associated with a loss of virulence. The present study aimed at investigating the genomic variations in the ORF150 region, and especially the deletion, in viruses isolated from carp populations of Indonesia. A screening of 236 fish from 43 different farms revealed a high prevalence of CyHV-3 (nearly 70%), both in symptomatic and asymptomatic common carp. However, in contrast with the results obtained in vitro, long read sequencing of the ORF150 region revealed a low level of genetic variations and the absence of the 1363-pb deletion. The complex interactions between the virus, the environment and the host, particularly the immune system, probably play an important role in this reduced variability.

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Section: Resultssupporting

confidence: 65%

Prevalence of Cyprinid herpesvirus 3 and ORF150 genomic variations in carp populations of Indonesia

Fuandila¹,

Lusiastuti²,

Yuhana

et al. 2023

Bulletin of the European Association of Fish Pathologists

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“…CEV DNA also could not be detected in gill samples of invasive round goby (Neobobius melanostomus) from various locations in Poland and Germany (Jin et al, 2020). In particular, the results of Matras's study (Matras et al, 2019) and the present data suggest that CEV primarily infects carp and koi and that replication of the virus most likely does not occur in other fish species.…”

Section: Discussioncontrasting

confidence: 53%

“…In addition, CEV‐ specific DNA could not be detected in gill samples from different fish species from a natural lake in Minnesota, USA where CEV‐related mortality in carp was observed (Tolo et al., 2021). CEV DNA also could not be detected in gill samples of invasive round goby ( Neobobius melanostomus ) from various locations in Poland and Germany (Jin et al., 2020). In particular, the results of Matras's study (Matras et al., 2019) and the present data suggest that CEV primarily infects carp and koi and that replication of the virus most likely does not occur in other fish species.…”

Section: Discussionmentioning

confidence: 99%

It is everywhere—A survey on the presence of carp edema virus in carp populations in Germany

Adamek

Heling

Bauer

et al. 2021

Transbounding Emerging Dis

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Carp edema virus (CEV) is the causative agent of koi sleepy disease (KSD), a serious gill disease affecting common carp, Cyprinus carpio, and its ornamental variety, koi. After recent detections of the virus in various countries around the world, KSD has emerged as a new global disease in carp. However, the prevalence of the infection in carp populations in a given geographical region has not been studied thoroughly. The present communication reports an investigation into the presence of CEV in carp and koi populations in Germany. For this purpose, gill samples collected from carp and koi populations suffering from gill diseases or collected for a routine examination of their health status were tested for the presence of CEV by PCR. In total, 651 fish samples from 401 carp or koi cases were examined in 2015 and 2016, additional 118 samples from previous studies were included in the examination. CEV was detected in archive samples from carp dating back to 2007, and in koi samples dating back to 2009. From 2015 to 2016, CEV was detected in 69% of cases from carp populations examined from the main carp‐producing areas in Germany, and in 41% of the examined cases from koi populations from all over Germany. Clinical KSD occurred mainly from April to June in carp populations at water temperatures ranging from 8 to 12°C and in koi populations at water temperatures ranging from 18 to 22°C. Most fish from clinically affected carp or koi populations harboured high virus loads of above 10,000 copies of CEV‐specific DNA per 250 ng DNA, while gills from fish of other fish species from the ponds, including goldfish, grass carp and European perch were found CEV negative or harboured a low virus load. A phylogenetic analysis revealed the presence of multiple CEV variants from genogroup I in carp and genogroup II in koi populations in Germany. Genetically identical genogroup I isolates were detected in carp from different geographical locations in Germany and in other European carp populations. Some German genogroup II variants were identical to variants previously recorded from koi in Asian and other European countries. The data presented here show that CEV is highly prevalent in German common carp and koi populations and implies the spreading of this virus by intense trading of common carp and koi without necessary risk mitigating measures. As infections with this virus may induce serious disease, CEV diagnostic should be included in health surveillance and disease monitoring programmes.

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The Upstream–Downstream Structural Disparity and Transboundary Challenges of Public Health

Marton

2023

Springer Water

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Detection of koi herpesvirus (KHV) and carp oedema virus (CEV) in invasive round goby, Neogobius melanostomus Pallas, 1814, from Poland and Germany

Cited by 7 publications

References 22 publications

Prevalence of Cyprinid herpesvirus 3 and ORF150 genomic variations in carp populations of Indonesia

Prevalence of Cyprinid herpesvirus 3 and ORF150 genomic variations in carp populations of Indonesia

It is everywhere—A survey on the presence of carp edema virus in carp populations in Germany

The Upstream–Downstream Structural Disparity and Transboundary Challenges of Public Health

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