Detection of High Prevalence of TEM/SHV/CTX-M Genes in ESBL Producing and Multidrug Resistant Klebsiella Pneumoniae and Klebsiella Oxytoca

Sb, Bauer; Pietropaoli, AP; Sn, Georas; Ma, Williams

doi:10.4172/2376-0311.1000130

J Clin Diagn Res

2016

DOI: 10.4172/2376-0311.1000130

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Detection of High Prevalence of TEM/SHV/CTX-M Genes in ESBL Producing and Multidrug Resistant Klebsiella Pneumoniae and Klebsiella Oxytoca

Bauer Sb¹,

AP Pietropaoli²,

Georas Sn³

et al.

Abstract: Background: Klebsiella spp. are Enterobacteriaceae frequently isolated from pathological specimens during urinary tract infections, bloodstream infection, and pus. They are becoming more and more resistant to antibiotics and challenging treatment options. β-lactamases are a great variety of enzymes capable of inducing resistance to β-lactams. The objective of this study was to identify extended-spectrum-β-lactamase (ESBL) genes in Klebsiella spp. strains isolated from various specimens in Lomé, Togo.

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Cited by 3 publications

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Distribution of CTX-M, TEM, SHV Beta-lactamase Gene among the Klebsiella pneumoniae Clinical Isolates from Tertiary Care Centre in Palakkad, Kerala

Kumaran¹,

Geetha²,

Baby³

2022

J. Pure Appl. Microbiol.

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Resistance against the routinely used antibiotics has reached a worrying level globally. Extended spectrum β-lactamases (ESBLs) production is the major mechanism of antimicrobial resistance. These ESBLs bacteria are resistance to penicillin, cephalosporins, monobactams. TEM1&2, CTX-M, SHV are the main ESBLs genes present in Klebsiella pneumoniae, which is produced by the alteration of amino acid in the active site. The aim of this study is to determine the prevalence of ESBL genes such as blaTEM 1&2, blaCTX-M and blaSHV. The present study was carried out from April 2019 to September 2019, a total of 121 K. pneumoniae isolates were collected and subjected to phenotypic study. Among these 19 isolated was ESBL positive, genes (blaSHV, blaTEM, blaCTX-M) were detected by conventional PCR method. blaTEM (100%) was the predominant gene detected flowed by CTX-M (68.42%) and SHV (57.89%). The highest level of antimicrobial resistance towards ampicillin (93.4%) followed by ceftriaxone (28.9%), cefotaxime (24.8%) and ciprofloxacin (22.3%). However, ESBL-producing isolates were showed resistance to ampicillin (100%) followed by ceftazidime (94.74%), cefotaxime (89.47%), amikacin and amoxicillin-clavulanic acid (68%). Antimicrobial resistance of bacteria is due to the genes, especially extended spectrum beta lactamase, which is widely found in members of Enterobacteriaceae. Nevertheless, there is a paucity of studies regarding the distribution of ESBL in K. pneumoniae in Palakkad Dist., Kerala. Hence the aim of the current study determines the distribution of ESBL genes in ESBL producing K. pneumoniae isolated from various clinical samples.

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Distribution of CTX-M, TEM, SHV Beta-lactamase Gene among the Klebsiella pneumoniae Clinical Isolates from Tertiary Care Centre in Palakkad, Kerala

Kumaran¹,

Geetha²,

Baby³

2022

J. Pure Appl. Microbiol.

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Prevalence of CTXM, SHV, TEM AND OXA Genes among Extended-Spectrum Beta-Lactamase Producing <i>Klebsiella pneumoniae</i> from Mukuru Slum, Kenya

Saisi¹,

Makobe²,

Kangongo³

et al. 2019

AiM

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Background: Extended spectrum beta lactamases (ESBLs) producing Enterobacteriaceae cause infections that are often reported in both hospital and community setting. These infections are on the increase and jeopardize the achievement of modern medicine because of their clinical implications. There is need for surveillance measures to be taken, both by the health care personnel and the community at large. Methodology: We examined 330 diarrhea stool samples from children below the age of 5 years and processed them. A total of 96 (29%) samples were identified as Klebsiella pneumoniae out of the bacteria isolated. Identification of ESBL was done and 42 K. pneumoniae isolates were tested for the occurrence of bla CTX-M, bla OXA, bl aTEM and bla SHV resistant genes by PCR, gel electrophoresis and visualized by UV illumination. Results: Our results revealed that bla CTXM was the most frequent ESBL type 42 (100%), followed by bla TEM in 41 (97.6%) isolates and bla SHVin38 (90.4%) of the isolates. None of the tested isolates were found to be encoding bla OXA. There was occurrence of more than one gene in most of the isolates. The double combination was detected in bla CTX-M/ bla TEM (9.5%) and bla CTXM/SHV (2.4%). A triple combination was noted bla-TEM/ bla SHV/ bla CTX-M (88%). Conclusion: Our results indicate that there is Presence of Beta lactam genes associated with antimicrobial resistance among the K. pneumoniae isolates from Mukuru Slum, Kenya. The predominant ESBL genotype in Mukuru slums, Kenya was bla CTX-M followed by bla TEM and bla SHV respectively. There is need for surveillance measures to be taken so as to control the spread among the community.

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Phenotypic and Molecular Detection of β-lactamase Genes blaTEM, blaCTX, and blaSHV Produced by Salmonella spp. Isolated from Poultry Meat

Ghazaei

2018

Gene Cell Tissue

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Detection of High Prevalence of TEM/SHV/CTX-M Genes in ESBL Producing and Multidrug Resistant Klebsiella Pneumoniae and Klebsiella Oxytoca

Cited by 3 publications

References 67 publications

Distribution of CTX-M, TEM, SHV Beta-lactamase Gene among the Klebsiella pneumoniae Clinical Isolates from Tertiary Care Centre in Palakkad, Kerala

Distribution of CTX-M, TEM, SHV Beta-lactamase Gene among the Klebsiella pneumoniae Clinical Isolates from Tertiary Care Centre in Palakkad, Kerala

Prevalence of CTXM, SHV, TEM AND OXA Genes among Extended-Spectrum Beta-Lactamase Producing <i>Klebsiella pneumoniae</i> from Mukuru Slum, Kenya

Phenotypic and Molecular Detection of β-lactamase Genes blaTEM, blaCTX, and blaSHV Produced by Salmonella spp. Isolated from Poultry Meat

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Detection of High Prevalence of TEM/SHV/CTX-M Genes in ESBL Producing and Multidrug Resistant Klebsiella Pneumoniae and Klebsiella Oxytoca

Cited by 3 publications

References 67 publications

Distribution of CTX-M, TEM, SHV Beta-lactamase Gene among the Klebsiella pneumoniae Clinical Isolates from Tertiary Care Centre in Palakkad, Kerala

Distribution of CTX-M, TEM, SHV Beta-lactamase Gene among the Klebsiella pneumoniae Clinical Isolates from Tertiary Care Centre in Palakkad, Kerala

Prevalence of CTXM, SHV, TEM AND OXA Genes among Extended-Spectrum Beta-Lactamase Producing &lt;i&gt;Klebsiella pneumoniae&lt;/i&gt; from Mukuru Slum, Kenya

Phenotypic and Molecular Detection of β-lactamase Genes blaTEM, blaCTX, and blaSHV Produced by Salmonella spp. Isolated from Poultry Meat

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Prevalence of CTXM, SHV, TEM AND OXA Genes among Extended-Spectrum Beta-Lactamase Producing <i>Klebsiella pneumoniae</i> from Mukuru Slum, Kenya